Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 282: C528-C537, 2002. First published October 24, 2001; doi:10.1152/ajpcell.00355.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
282/3/C528    most recent
00355.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huey, K. A.
Right arrow Articles by Baldwin, K. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huey, K. A.
Right arrow Articles by Baldwin, K. M.
Vol. 282, Issue 3, C528-C537, March 2002

Transcriptional regulation of the type I myosin heavy chain promoter in inactive rat soleus

K. A. Huey1, R. R. Roy3, F. Haddad1, V. R. Edgerton2,3, and K. M. Baldwin1

1 Department of Physiology and Biophysics, University of California, Irvine, Irvine 92697; and 2 Department of Physiological Science, and 3 Brain Research Institute, University of California, Los Angeles, Los Angeles, California 90095-1761

Chronic muscle inactivity with spinal cord isolation (SI) decreases expression of slow type I myosin heavy chain (MHC) while increasing expression of the faster MHC isoforms, primarily IIx. The purpose of this study was to determine whether type I MHC downregulation in the soleus muscle of SI rats is regulated transcriptionally and to identify cis-acting elements or regions of the rat type I MHC gene promoter involved in this response. One week of SI significantly decreased in vivo activity of the -3500-, -408-, -299-, -215-, and -171-bp type I MHC promoters. The activity of all tested deletions of the type I MHC promoter, relative to the human skeletal alpha -actin promoter, were significantly reduced in the SI soleus, except activity of the -171-bp promoter, which increased. Mutation of the beta e3 element (-214/-190 bp) in the -215- and -408-bp promoters and deletion of this element (-171-bp promoter) attenuated type I downregulation with SI. Gel mobility shift assays demonstrated a decrease in transcription enhancer factor-1 binding to the beta e3 element with SI, despite an increase in total binding to this region. These results demonstrate that type I MHC downregulation with SI is transcriptionally regulated and suggest that interactions between transcription enhancer factor-1 and the beta e3 element are likely involved in this response.

beta e3 DNA regulatory element; transcription enhancer factor-1; spinal cord isolation; chronic muscle inactivity


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
H. Choi, P.-J. I. Selpides, M. M. Nowell, and B. C. Rourke
Functional overload in ground squirrel plantaris muscle fails to induce myosin isoform shifts
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2009; 297(3): R578 - R586.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. E. Pandorf, F. Haddad, A. X. Qin, and K. M. Baldwin
IIx myosin heavy chain promoter regulation cannot be characterized in vivo by direct gene transfer
Am J Physiol Cell Physiol, October 1, 2007; 293(4): C1338 - C1346.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A. Raffaello, P. Laveder, C. Romualdi, C. Bean, L. Toniolo, E. Germinario, A. Megighian, D. Danieli-Betto, C. Reggiani, and G. Lanfranchi
Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling
Physiol Genomics, March 13, 2006; 25(1): 60 - 74.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. M. Giger, F. Haddad, A. X. Qin, M. Zeng, and K. M. Baldwin
Effect of unloading on type I myosin heavy chain gene regulation in rat soleus muscle
J Appl Physiol, April 1, 2005; 98(4): 1185 - 1194.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. A. Shanely, D. Van Gammeren, K. C. DeRuisseau, A. M. Zergeroglu, M. J. McKenzie, K. E. Yarasheski, and S. K. Powers
Mechanical Ventilation Depresses Protein Synthesis in the Rat Diaphragm
Am. J. Respir. Crit. Care Med., November 1, 2004; 170(9): 994 - 999.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F. Haddad, R. R. Roy, H. Zhong, V. R. Edgerton, and K. M. Baldwin
Atrophy responses to muscle inactivity. I. Cellular markers of protein deficits
J Appl Physiol, August 1, 2003; 95(2): 781 - 790.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
F. Haddad, R. R. Roy, H. Zhong, V. R. Edgerton, and K. M. Baldwin
Atrophy responses to muscle inactivity. II. Molecular markers of protein deficits
J Appl Physiol, August 1, 2003; 95(2): 791 - 802.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. A. Huey, F. Haddad, A. X. Qin, and K. M. Baldwin
Transcriptional regulation of the type I myosin heavy chain gene in denervated rat soleus
Am J Physiol Cell Physiol, March 1, 2003; 284(3): C738 - C748.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. W. Tsika, J. McCarthy, N. Karasseva, Y. Ou, and G. L. Tsika
Divergence in species and regulatory role of beta -myosin heavy chain proximal promoter muscle-CAT elements
Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1761 - C1775.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. J. Talmadge, R. R. Roy, V. J. Caiozzo, and V. R. Edgerton
Mechanical properties of rat soleus after long-term spinal cord transection
J Appl Physiol, October 1, 2002; 93(4): 1487 - 1497.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online