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Am J Physiol Cell Physiol 282: C366-C373, 2002; doi:10.1152/ajpcell.00187.2000
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Vol. 282, Issue 2, C366-C373, February 2002

Circadian rhythm in intracellular Clminus activity of acutely dissociated neurons of suprachiasmatic nucleus

Masahiko Shimura1, Norio Akaike2, and Nobutoshi Harata3

1 Department of Ophthalmology, Tohoku University School of Medicine, Sendai 980-8574, Japan; 2 Cellular and System Physiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan; and 3 Department of Molecular and Cellular Physiology, Beckman Center, Stanford University School of Medicine, Stanford, California 94305-5345

A link between the circadian rhythm and the function of Cl--permeable gamma -aminobutyric acid (GABA) type A (GABAA) receptors on suprachiasmatic nucleus (SCN) neurons was studied by measuring intracellular activity of Cl- (aCl<UP><SUB>i</SUB><SUP>−</SUP></UP>) at different times during a circadian cycle in SCN neurons acutely dissociated from rat brains. To measure aCl<UP><SUB>i</SUB><SUP>−</SUP></UP>, the voltage-clamp mode of the gramicidin-perforated patch-clamp technique was used, and reversal potential of GABA-induced currents (EGABA) was converted to aCl<UP><SUB>i</SUB><SUP>−</SUP></UP>. Measured aCl<UP><SUB>i</SUB><SUP>−</SUP></UP> was significantly higher at around noon (20.1 ± 1.4 mM) than at three other time zones of a circadian cycle (means ranging from 11.6 to 14.3 mM). Chord conductance of GABA-induced currents showed no circadian changes, indicating a lack of circadian changes in the number or single-channel conductance of GABAA receptors. These results suggest that aCl<UP><SUB>i</SUB><SUP>−</SUP></UP> participates in modulating GABAA receptor functions on SCN neurons during the circadian rhythm.

gamma -aminobutyric acid; gamma -aminobutyric acid type A receptor; gramicidin-perforated patch clamp; rat


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