| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Physiology and Biophysics and Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294 - 0005
ATP and its metabolites regulate vascular tone; however, the sources of the ATP released in vascular beds are ill defined. As such, we tested the hypothesis that all limbs of an extracellular purinergic signaling system are present in vascular endothelial cells: ATP release, ATP receptors, and ATP receptor-triggered signal transduction. Primary cultures of human endothelial cells derived from multiple blood vessels were grown as monolayers and studied using a bioluminescence detection assay for ATP released into the medium. ATP is released constitutively and exclusively across the apical membrane under basal conditions. Hypotonic challenge or the calcium agonists ionomycin and thapsigargin stimulate ATP release in a reversible and regulated manner. To assess expression of P2X purinergic receptor channel subtypes (P2XRs), we performed degenerate RT-PCR, sequencing of the degenerate P2XR product, and immunoblotting with P2XR subtype-specific antibodies. Results revealed that P2X4 and P2X5 are expressed abundantly by endothelial cell primary cultures derived from multiple blood vessels. Together, these results suggest that components of an autocrine purinergic signaling loop exist in the endothelial cell microvasculature that may allow for "self-regulation" of endothelial cell function and modulation of vascular tone.
purinergic receptors; cytosolic calcium; ectoadenosinetriphosphatases; exocytosis; nitric oxide
This article has been cited by other articles:
|
|
 |
|
  H. N. Woodward, A. Anwar, S. Riddle, L. Taraseviciene-Stewart, M. Fragoso, K. R. Stenmark, and E. V. Gerasimovskaya PI3K, Rho, and ROCK play a key role in hypoxia-induced ATP release and ATP-stimulated angiogenic responses in pulmonary artery vasa vasorum endothelial cells Am J Physiol Lung Cell Mol Physiol, November 1, 2009; 297(5): L954 - L964. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. A. Kolosova, T. Mirzapoiazova, L. Moreno-Vinasco, S. Sammani, J. G. N. Garcia, and A. D. Verin Protective effect of purinergic agonist ATP{gamma}S against acute lung injury Am J Physiol Lung Cell Mol Physiol, February 1, 2008; 294(2): L319 - L324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A Fitsanakis, G. Piccola, A. P. Marreilha dos Santos, J. L Aschner, and M. Aschner Putative proteins involved in manganese transport across the blood-brain barr 1ier Human and Experimental Toxicology, April 1, 2007; 26(4): 295 - 302. [Abstract] [PDF]
|
|
|
|
 |
|
 D. Gunduz, S. A. Kasseckert, F. V. Hartel, M. Aslam, Y. Abdallah, M. Schafer, H. M. Piper, T. Noll, and C. Schafer Accumulation of extracellular ATP protects against acute reperfusion injury in rat heart endothelial cells Cardiovasc Res, September 1, 2006; 71(4): 764 - 773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Jacobson, S. M. Dudek, P. A. Singleton, I. A. Kolosova, A. D. Verin, and J. G. N. Garcia Endothelial cell barrier enhancement by ATP is mediated by the small GTPase Rac and cortactin. Am J Physiol Lung Cell Mol Physiol, August 1, 2006; 291(2): L289 - L295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Peti-Peterdi Calcium wave of tubuloglomerular feedback Am J Physiol Renal Physiol, August 1, 2006; 291(2): F473 - F480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Lee, S. H. Park, and H. J. Han ATP stimulates Na+-glucose cotransporter activity via cAMP and p38 MAPK in renal proximal tubule cells Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1268 - C1276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Liang, A. Zsembery, and E. M. Schwiebert RNA interference targeted to multiple P2X receptor subtypes attenuates zinc-induced calcium entry Am J Physiol Cell Physiol, August 1, 2005; 289(2): C388 - C396. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Sylte, C. J. Kuckleburg, T. J. Inzana, P. J. Bertics, and C. J. Czuprynski Stimulation of P2X receptors enhances lipooligosaccharide-mediated apoptosis of endothelial cells J. Leukoc. Biol., June 1, 2005; 77(6): 958 - 965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. F. Okada, W. K. O'Neal, P. Huang, R. A. Nicholas, L. E. Ostrowski, W. J. Craigen, E. R. Lazarowski, and R. C. Boucher Voltage-dependent Anion Channel-1 (VDAC-1) Contributes to ATP Release and Cell Volume Regulation in Murine Cells J. Gen. Physiol., October 25, 2004; 124(5): 513 - 526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Kochukov and A. K. Ritchie A P2X7 receptor stimulates plasma membrane trafficking in the FRTL rat thyrocyte cell line Am J Physiol Cell Physiol, October 1, 2004; 287(4): C992 - C1002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Glass and D. O. Bates The role of endothelial cell Ca2+ store release in the regulation of microvascular permeability in vivo Exp Physiol, July 1, 2004; 89(4): 343 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ahmad, A. Ahmad, M. Ghosh, C. C. Leslie, and C. W. White Extracellular ATP-mediated Signaling for Survival in Hyperoxia-induced Oxidative Stress J. Biol. Chem., April 16, 2004; 279(16): 16317 - 16325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. North P2X3 receptors and peripheral pain mechanisms J. Physiol., January 15, 2004; 554(2): 301 - 308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Wang, M. Andersson, L. Karlsson, M.-A. Watson, D. J. Cousens, S. Jern, and D. Erlinge Increased Mitogenic and Decreased Contractile P2 Receptors in Smooth Muscle Cells by Shear Stress in Human Vessels With Intact Endothelium Arterioscler Thromb Vasc Biol, August 1, 2003; 23(8): 1370 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S. Sprague, J. J. Olearczyk, D. M. Spence, A. H. Stephenson, R. W. Sprung, and A. J. Lonigro Extracellular ATP signaling in the rabbit lung: erythrocytes as determinants of vascular resistance Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H693 - H700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamamoto, T. Sokabe, N. Ohura, H. Nakatsuka, A. Kamiya, and J. Ando Endogenously released ATP mediates shear stress-induced Ca2+ influx into pulmonary artery endothelial cells Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H793 - H803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Joseph, M. R. Buchakjian, and G. R. Dubyak Colocalization of ATP Release Sites and Ecto-ATPase Activity at the Extracellular Surface of Human Astrocytes J. Biol. Chem., June 20, 2003; 278(26): 23331 - 23342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Aleu, M. Martin-Satue, P. Navarro, I. P. de Lara, L. Bahima, J. Marsal, and C. Solsona Release of ATP induced by hypertonic solutions in Xenopus oocytes J. Physiol., February 15, 2003; 547(1): 209 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. North Molecular Physiology of P2X Receptors Physiol Rev, October 1, 2002; 82(4): 1013 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Dubyak Focus on "Extracellular ATP signaling and P2X nucleotide receptors in monolayers of primary human vascular endothelial cells" Am J Physiol Cell Physiol, February 1, 2002; 282(2): C242 - C244. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
