Am J Physiol Cell Physiol AJP: Regulatory, Integrative and Comparative Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 282: C263-C270, 2002; doi:10.1152/ajpcell.00357.2001
0363-6143/02 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schwacha, M. G.
Right arrow Articles by Chaudry, I. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schwacha, M. G.
Right arrow Articles by Chaudry, I. H.
Vol. 282, Issue 2, C263-C270, February 2002

Cyclooxygenase 2-mediated suppression of macrophage interleukin-12 production after thermal injury

Martin G. Schwacha, Chun-Shiang Chung, Alfred Ayala, Kirby I. Bland, and Irshad H. Chaudry

Center for Surgical Research, Department of Surgery, University of Alabama at Birmingham, Birmingham, Alabama 35294; and Division of Surgical Research, Department of Surgery, Brown University School of Medicine, Providence, Rhode Island 02903

Macrophage (Mphi ) prostaglandin (PG)E2 production has been implicated in immunosuppression and increased susceptibility to sepsis after thermal injury. Deficient interleukin (IL)-12 production has also been implicated in these postburn complications. The present study examined the relationship between Mphi cyclooxygenase (COX)-2 activity and IL-12 production after thermal injury. C57BL/6 female mice were subjected to a 25% total body surface area full-thickness burn. Mphi were isolated 7 days later, or the mice were subjected to sepsis by cecal ligation and puncture (CLP). IL-12 production by Mphi from injured mice was suppressed by >50%, whereas COX-2 expression and PGE2 production were increased twofold. The COX-2 inhibitor NS-398 suppressed PGE2 production and normalized IL-12 production in the injury group, whereas it had no effect on IL-10 production. Injured mice subjected to CLP had lower IL-12 plasma levels compared with sham-treated mice subjected to CLP. NS-398 treatment prevented the suppression in plasma IL-12 levels in the injury group. Thus elevated Mphi COX-2 activity, independent of IL-10, suppresses Mphi IL-12 production after thermal injury and may play an important role in the observed immunosuppression under such conditions.

prostaglandin E2; interleukin-10; immunosuppression; sepsis; burns


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
C. P. Schneider, M. G. Schwacha, and I. H. Chaudry
Impact of sex and age on bone marrow immune responses in a murine model of trauma-hemorrhage
J Appl Physiol, January 1, 2007; 102(1): 113 - 121.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
R. Gurfinkel, D. Czeiger, A. Douvdevani, Y. Shapira, A. A. Artru, Y. Sufaro, J. Mazar, and G. Shaked
Ketamine improves survival in burn injury followed by sepsis in rats.
Anesth. Analg., August 1, 2006; 103(2): 396 - 402, table of contents.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. L. Palmer, J. M. Tulley, E. J. Kovacs, R. L. Gamelli, M. Taniguchi, and D. E. Faunce
Injury-Induced Suppression of Effector T Cell Immunity Requires CD1d-Positive APCs and CD1d-Restricted NKT Cells
J. Immunol., July 1, 2006; 177(1): 92 - 99.
[Abstract] [Full Text] [PDF]

Home page
 J Intensive Care MedHome page
J. W. Smith, R. L. Gamelli, S. B. Jones, and R. Shankar
Immunologic Responses to Critical Injury and Sepsis
J Intensive Care Med, May 1, 2006; 21(3): 160 - 172.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online