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1 Department of Medicine, Division of Nephrology and Indiana Center for Biological Microscopy, Indiana University School of Medicine, Indianapolis 46202-5116; and 2 Department of Computer and Information Science, Indiana University-Purdue University at Indianapolis, Indianapolis, Indiana 46202-5132
Confocal and two-photon fluorescence microscopy have advanced the exploration of complex, three-dimensional biological structures at submicron resolution. We have developed a voxel-based three-dimensional (3-D) imaging program (Voxx) capable of near real-time rendering that runs on inexpensive personal computers. This low-cost interactive 3-D imaging system provides a powerful tool for analyzing complex structures in cells and tissues and encourages a more thorough exploration of complex biological image data.
confocal; three-dimensional; two-photon; voxels
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