Am J Physiol Cell Physiol AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 281: C1825-C1836, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bridges, C. C.
Right arrow Articles by Smith, S. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bridges, C. C.
Right arrow Articles by Smith, S. B.
Vol. 281, Issue 6, C1825-C1836, December 2001

Regulation of taurine transporter expression by NO in cultured human retinal pigment epithelial cells

Christy C. Bridges1, M. Shamsul Ola1, Puttur D. Prasad2, Amira El-Sherbeny1, Vadivel Ganapathy2,3, and Sylvia B. Smith1,4

Departments of 1 Cellular Biology and Anatomy, 2 Obstetrics and Gynecology, 3 Biochemistry and Molecular Biology, and 4 Ophthalmology, Medical College of Georgia, Augusta, Georgia 30912

Taurine is actively transported at the retinal pigment epithelial (RPE) apical membrane in an Na+- and Cl--dependent manner. Diabetes may alter the function of the taurine transporter. Because nitric oxide (NO) is a molecule implicated in the pathogenesis of diabetes, we asked whether NO would alter the activity of the taurine transporter in cultured ARPE-19 cells. The activity of the transporter was stimulated in the presence of the NO donor 3-morpholinosydnonimine. The stimulatory effects of 3-morpholinosydnonimine were not observed during the initial 16-h treatment; however, stimulation of taurine uptake was elevated dramatically above control values with 20- and 24-h treatments. Kinetic analysis revealed that the stimulation was associated with an increase in the maximal velocity of the transporter with no significant change in the substrate affinity. The NO-induced increase in taurine uptake was inhibited by actinomycin D and cycloheximide. RT-PCR analysis and nuclear run-on assays provided evidence for upregulation of the transporter gene. This study provides the first evidence of an increase in taurine transporter gene expression in human RPE cells cultured under conditions of elevated levels of NO.

cell culture


This article has been cited by other articles:


Home page
 Drug Metab. Dispos.Home page
H.-J. Maeng, M.-H. Kim, H.-E. Jin, S. M. Shin, T. Tsuruo, S. G. Kim, D.-D. Kim, C.-K. Shim, and S.-J. Chung
Functional Induction of P-glycoprotein in the Blood-Brain Barrier of Streptozotocin-Induced Diabetic Rats: Evidence for the Involvement of Nuclear Factor-{kappa}B, a Nitrosative Stress-Sensitive Transcription Factor, in the Regulation
Drug Metab. Dispos., November 1, 2007; 35(11): 1996 - 2005.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. Roos, T. L. Powell, and T. Jansson
Human placental taurine transporter in uncomplicated and IUGR pregnancies: cellular localization, protein expression, and regulation
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2004; 287(4): R886 - R893.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. E. Mann, D. L. Yudilevich, and L. Sobrevia
Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells
Physiol Rev, January 1, 2003; 83(1): 183 - 252.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online