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Am J Physiol Cell Physiol 281: C1785-C1796, 2001;
0363-6143/01 $5.00
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Vol. 281, Issue 6, C1785-C1796, December 2001

Developmental changes in ryanodine- and IP3-sensitive Ca2+ pools in ovine basilar artery

S. M. Nauli, J. M. Williams, S. E. Akopov, L. Zhang, and W. J. Pearce

Department of Physiology, Center for Perinatal Biology, Loma Linda University School of Medicine, Loma Linda, California 92350

To explore the hypothesis that cerebrovascular maturation alters ryanodine- and inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ pool sizes, we measured total intracellular Ca2+ with 45Ca and the fractions of intracellular Ca2+ released by IP3 and/or caffeine in furaptra-loaded permeabilized basilar arteries from nonpregnant adult and term fetal (139-141 days) sheep. Ca2+ mass (nmol/mg dry weight) was similar in adult (1.60 ± 0.18) and fetal (1.71 ± 0.16) arteries in the pool sensitive to IP3 alone but was significantly lower for adult (0.11 ± 0.01) than for fetal (1.22 ± 0.11) arteries in the pool sensitive to ryanodine alone. The pool sensitive to both ryanodine and IP3 was also smaller in adult (0.14 ± 0.01) than in fetal (0.85 ± 0.08) arteries. Because the Ca2+ fraction in the ryanodine-IP3 pool was small in both adult (5 ± 1%) and fetal (7 ± 4%) arteries, the IP3 and ryanodine pools appear to be separate in these arteries. However, the pool sensitive to neither IP3 nor ryanodine was 10-fold smaller in adult (0.87 ± 0.10) than in fetal (8.78 ± 0.81) arteries, where it accounted for 72% of total intracellular membrane-bound Ca2+. Thus, during basilar artery maturation, intracellular Ca2+ mass plummets in noncontractile pools, decreases modestly in ryanodine-sensitive pools, and remains constant in IP3-sensitive pools. In addition, age-related increases in IP3 efficacy must involve factors other than IP3 pool size alone.

cerebral arteries; cerebrovascular circulation; furaptra; maturation; sarcoplasmic reticulum


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