Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 281: C1635-C1641, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Donahue, S. W.
Right arrow Articles by Donahue, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Donahue, S. W.
Right arrow Articles by Donahue, H. J.
Vol. 281, Issue 5, C1635-C1641, November 2001

Flow-induced calcium oscillations in rat osteoblasts are age, loading frequency, and shear stress dependent

Seth W. Donahue1, Christopher R. Jacobs2,3, and Henry J. Donahue4

1 Department of Biomedical Engineering, Michigan Technological University, Houghton, Michigan 49931-1295; 2 Rehab Research and Development Center, Veterans Affairs Medical Center, Palo Alto 94304-1200; 3 Biomechanical Engineering Division, Stanford University, Stanford, California 94305-3030; and 4 Musculoskeletal Research Laboratory, Department of Orthopaedics and Rehabilitation and Department of Cellular and Molecular Physiology, The Pennsylvania State University, Hershey, Pennsylvania 17033

Bone adaptation to mechanical loading is dependent on age and the frequency and magnitude of loading. It is believed that load-induced fluid flow in the porous spaces of bone is an important signal that influences bone cell metabolism and bone adaptation. We used fluid flow-induced shear stress as a mechanical stimulus to study intracellular calcium (Ca<UP><SUB>i</SUB><SUP>2+</SUP></UP>) signaling in rat osteoblastic cells (ROB) isolated from young, mature, and old animals. Fluid flow produced higher magnitude and more abundant [Ca2+]i oscillations than spontaneous oscillations, suggesting that flow-induced Ca<UP><SUB>i</SUB><SUP>2+</SUP></UP> signaling encodes a different cellular message than spontaneous oscillations. ROB from old rats showed less basal [Ca2+]i activity and were less responsive to fluid flow. Cells were more responsive to 0.2 Hz than to 1 or 2 Hz and to 2 Pa than to 1 Pa. These data suggest that the frequency and magnitude of mechanical loading may be encoded by the percentage of cells displaying [Ca2+]i oscillations but that the ability to transduce this information may be altered with age.

mechanotransduction; osteoblast; calcium signaling; bone adaptation


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
S. Srinivasan, B. J. Ausk, S. L. Poliachik, S. E. Warner, T. S. Richardson, and T. S. Gross
Rest-inserted loading rapidly amplifies the response of bone to small increases in strain and load cycles
J Appl Physiol, May 1, 2007; 102(5): 1945 - 1952.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. Zhang, S. M. Tanaka, H. Jiang, M. Su, and H. Yokota
Diaphyseal bone formation in murine tibiae in response to knee loading
J Appl Physiol, May 1, 2006; 100(5): 1452 - 1459.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. M. Ponik and F. M. Pavalko
Formation of focal adhesions on fibronectin promotes fluid shear stress induction of COX-2 and PGE2 release in MC3T3-E1 osteoblasts
J Appl Physiol, July 1, 2004; 97(1): 135 - 142.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. M. LaMothe and R. F. Zernicke
Rest insertion combined with high-frequency loading enhances osteogenesis
J Appl Physiol, May 1, 2004; 96(5): 1788 - 1793.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
D. E. Lieberman, O. M. Pearson, J. D. Polk, B. Demes, and A. W. Crompton
Optimization of bone growth and remodeling in response to loading in tapered mammalian limbs
J. Exp. Biol., September 15, 2003; 206(18): 3125 - 3138.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online