Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 281: C1504-C1511, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (16)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jayaraman, S.
Right arrow Articles by Verkman, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jayaraman, S.
Right arrow Articles by Verkman, A. S.
Vol. 281, Issue 5, C1504-C1511, November 2001

Airway surface liquid pH in well-differentiated airway epithelial cell cultures and mouse trachea

Sujatha Jayaraman, Yuanlin Song, and A. S. Verkman

Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0521

Airway surface liquid (ASL) pH has been proposed to be important in the pathophysiology of cystic fibrosis, asthma, and cough. Ratio image analysis was used to measure pH in the ASL after staining with the fluorescent pH indicator 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF)-dextran. ASL pH in bovine airway cell cultures grown at an air-liquid interface was 6.98 ± 0.06 in the absence and 6.81 ± 0.04 in the presence of HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>/CO2. Steady-state ASL pH changed in parallel to changes in bath pH and was acidified by Na+ or Cl- replacement but was not affected by the inhibitors amiloride, glibenclamide, or 4,4'-dinitrostilbene-2,2'-disulfonic acid. In response to sudden acidification or alkalization of the ASL by ~0.4 pH units by HCl/NaOH, ASL pH recovered to its initial value at a rate of 0.035 pH units/min (-HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>) and 0.060 pH units/min (+HCO<UP><SUB>3</SUB><SUP>−</SUP></UP>); the pH recovery rate was reduced by amiloride and H2DIDS. In anesthetized mice in which the trachea was surgically exposed for measurement of BCECF-dextran fluorescence through the translucent tracheal wall, ASL pH was 7.14 ± 0.01. ASL pH was sensitive to changes in blood pH created by metabolic (HCl or NaHCO3 infusion) or respiratory (hyperventilation, hypoventilation) mechanisms. ASL pH is thus primarily determined by basolateral fluid pH, and H+/OH- transport between the ASL and basolateral fluid involves amiloride-sensitive Na+/H+ exchange and stilbene-sensitive Cl-/HCO<UP><SUB>3</SUB><SUP>−</SUP></UP> exchange. The rapid response of ASL pH to changes in systemic acid-base status may contribute to airway hypersensitivity in asthma and other airway diseases.

cystic fibrosis; acidification; trachea; fluorescence microscopy


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
T. E. Machen
Innate immune response in CF airway epithelia: hyperinflammatory?
Am J Physiol Cell Physiol, August 1, 2006; 291(2): C218 - C230.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Song, D. Salinas, D. W. Nielson, and A. S. Verkman
Hyperacidity of secreted fluid from submucosal glands in early cystic fibrosis
Am J Physiol Cell Physiol, March 1, 2006; 290(3): C741 - C749.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. L. Miller, P. Zhang, M. Smith, V. Beaulieu, T. G. Paunescu, D. Brown, S. Breton, and R. D. Nelson
V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice
Am J Physiol Cell Physiol, May 1, 2005; 288(5): C1134 - C1144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. L. Clarke, L. R. Gawenis, E. M. Bradford, L. M. Judd, K. T. Boyle, J. E. Simpson, G. E. Shull, H. Tanabe, A. J. Ouellette, C. L. Franklin, et al.
Abnormal Paneth cell granule dissolution and compromised resistance to bacterial colonization in the intestine of CF mice
Am J Physiol Gastrointest Liver Physiol, June 1, 2004; 286(6): G1050 - G1058.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
D. McShane, J.C. Davies, M.G. Davies, A. Bush, D.M. Geddes, and E.W.F.W. Alton
Airway surface pH in subjects with cystic fibrosis
Eur. Respir. J., January 1, 2003; 21(1): 37 - 42.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. S. Verkman, Y. Song, and J. R. Thiagarajah
Role of airway surface liquid and submucosal glands in cystic fibrosis lung disease
Am J Physiol Cell Physiol, January 1, 2003; 284(1): C2 - C15.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online