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Am J Physiol Cell Physiol 281: C1318-C1325, 2001;
0363-6143/01 $5.00
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Vol. 281, Issue 4, C1318-C1325, October 2001

Localization of the urea transporter UT-B protein in human and rat erythrocytes and tissues

Richard T. Timmer1,*, Janet D. Klein2,*, Serena M. Bagnasco3, John J. Doran2, Jill W. Verlander4, Robert B. Gunn1, and Jeff M. Sands2

Departments of 1 Physiology, 2 Medicine (Renal Division), and 3 Pathology, Emory University School of Medicine, Atlanta, Georgia 30322; and 4 Department of Medicine, College of Medicine Electron Microscopy Core Facility, University of Florida College of Medicine, Gainesville, Florida 32610

A new polyclonal antibody to the human erythrocyte urea transporter UT-B detects a broad band between 45 and 65 kDa in human erythrocytes and between 37 and 51 kDa in rat erythrocytes. In human erythrocytes, the UT-B protein is the Kidd (Jk) antigen, and Jk(a+b+) erythrocytes express the 45- to 65-kDa band. However, in Jk null erythrocytes [Jk(a-b-)], only a faint band at 55 kDa is detected. In kidney medulla, a broad band between 41 and 54 kDa, as well as a larger band at 98 kDa, is detected. Human and rat kidney show UT-B staining in nonfenestrated endothelial cells in descending vasa recta. UT-B protein and mRNA are detected in rat brain, colon, heart, liver, lung, and testis. When kidney medulla or liver proteins are analyzed with the use of a native gel, only a single protein band is detected. UT-B protein is detected in cultured bovine endothelial cells. We conclude that UT-B protein is expressed in more rat tissues than previously reported, as well as in erythrocytes.

kidney; brain; liver; testis; lung; heart; colon; Kidd antigen; endothelial cells

* R. T. Timmer and J. D. Klein contributed equally to this work.




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