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1 Exercise Physiology and Metabolism Laboratory, Department of Physiology, The University of Melbourne, Parkville, Victoria 3010; and 2 Department of Pathology and Immunology, Monash Medical School, Prahran, Victoria 3181, Australia
To examine the effect of exercise and
adrenergic blockade on lymphocyte cytokine production, six men ingested
either a placebo (control) or an 
- (prazosin hydrochloride) and
-adrenoceptor antagonist (timolol malate) capsule (blockade, or BLK)
2 h before performing 19 ± 1 min of supine bicycle exercise
at 78 ± 3% peak pulmonary uptake. Blood was collected before and
after exercise, stimulated with phorbol 12-myristate 13-acetate and
ionomycin, and surface stained for T (CD3+) and natural
killer [NK (CD3
CD56+)] lymphocyte surface
antigens. Cells were permeabilized, stained for the intracellular
cytokines interleukin (IL)-2 and interferon (IFN)-
, and analyzed
using flow cytometry. BLK had no effect on the resting concentration of
stimulated cytokine-positive T and NK lymphocytes or the amount of
cytokine they were producing. Exercise resulted in an increase (P
< 0.05) in the concentration of stimulated T and NK lymphocytes
producing cytokines in the circulation, but these cells produced less
(P < 0.05) cytokine post- compared with preexercise.
BLK attenuated (P < 0.05) the elevation in the
concentration of lymphocytes producing cytokines during exercise;
however, BLK did not affect the amount of IL-2 and IFN- produced.
These results suggest that adrenergic stimulation contributes to the
exercise-induced increase in the concentration of lymphocytes in the
circulation; however, it does not appear to be responsible for the
exercise-induced suppression in cytokine production.
T cells; natural killer cells; interleukin-2; interferon-
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