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Am J Physiol Cell Physiol 281: C876-C885, 2001;
0363-6143/01 $5.00
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Vol. 281, Issue 3, C876-C885, September 2001

Regulation of cytoplasmic calcium levels by two nitric oxide receptors

Charles L. Bowman1,2, Lori Yohe1, and James W. Lohr1,3

1 Buffalo Institute for Medical Research, Veterans Administration Medical Center, Buffalo 14215-1129; and 2 Department of Physiology and Biophysical Sciences, and 3 Departments of Medicine and Pharmacology, University at Buffalo, Buffalo, New York 14214-3005

We examined the effects of dissolved nitric oxide (NO) gas on cytoplasmic calcium levels ([Ca2+]i) in C6 glioma cells under anoxic conditions. The maximum elevation (27 ± 3 nM) of [Ca2+]i was reached at 10 µM NO. A second application of NO was ineffective if the first was >0.5 µM. The NO donor diethylamine/NO mimicked the effects of NO. Acute exposure of the cells to low calcium levels was without effect on the NO-evoked response. Thapsigargin (TG) increased [Ca2+]i and was less effective if cells were pretreated with NO. Hemoglobin inhibited the effects of NO at a molar ratio of 10:1. 8-Bromo-cGMP was without effect on the NO-evoked response. If cells were pretreated with TG or exposed chronically to nominal amounts of calcium, NO decreased [Ca2+]i. The results suggest that C6 glioma cells have two receptors for NO. One receptor (NOA) elevates [Ca2+]i and resides on the endoplasmic reticulum (ER). The other receptor (NOB) decreases [Ca2+]i and resides on the plasmalemma or the ER. The latter receptor dominates when the level of calcium within intracellular stores is diminished.

neuroglia; glioma; astrocytes


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J. Zhang, S.-L. Xia, E. R. Block, and J. M. Patel
NO upregulation of a cyclic nucleotide-gated channel contributes to calcium elevation in endothelial cells
Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1080 - C1089.
[Abstract] [Full Text] [PDF]


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