Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 281: C1005-C1013, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (47)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lyall, V.
Right arrow Articles by DeSimone, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lyall, V.
Right arrow Articles by DeSimone, J. A.
Vol. 281, Issue 3, C1005-C1013, September 2001

Decrease in rat taste receptor cell intracellular pH is the proximate stimulus in sour taste transduction

Vijay Lyall1, Rammy I. Alam1, Duy Q. Phan1, Glenn L. Ereso1, Tam-Hao T. Phan1, Shahbaz A. Malik1, Marshall H. Montrose2, Shaoyou Chu2, Gerard L. Heck1, George M. Feldman1,3, and John A. DeSimone1

1 Department of Physiology, Virginia Commonwealth University, Richmond 23298-0551, 3 McGuire Veterans Affairs Medical Center, Richmond, Virginia 23249; and 2 Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis, Indiana 46202-5120

Taste receptor cells (TRCs) respond to acid stimulation, initiating perception of sour taste. Paradoxically, the pH of weak acidic stimuli correlates poorly with the perception of their sourness. A fundamental issue surrounding sour taste reception is the identity of the sour stimulus. We tested the hypothesis that acids induce sour taste perception by penetrating plasma membranes as H+ ions or as undissociated molecules and decreasing the intracellular pH (pHi) of TRCs. Our data suggest that taste nerve responses to weak acids (acetic acid and CO2) are independent of stimulus pH but strongly correlate with the intracellular acidification of polarized TRCs. Taste nerve responses to CO2 were voltage sensitive and were blocked with MK-417, a specific blocker of carbonic anhydrase. Strong acids (HCl) decrease pHi in a subset of TRCs that contain a pathway for H+ entry. Both the apical membrane and the paracellular shunt pathway restrict H+ entry such that a large decrease in apical pH is translated into a relatively small change in TRC pHi within the physiological range. We conclude that a decrease in TRC pHi is the proximate stimulus in rat sour taste transduction.

hydrogen ion conductance; intracellular signaling; chorda tympani; acid stimulation


This article has been cited by other articles:


Home page
 ScienceHome page
J. Chandrashekar, D. Yarmolinsky, L. von Buchholtz, Y. Oka, W. Sly, N. J. P. Ryba, and C. S. Zuker
The Taste of Carbonation
Science, October 16, 2009; 326(5951): 443 - 445.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
R. Yoshida, A. Miyauchi, T. Yasuo, M. Jyotaki, Y. Murata, K. Yasumatsu, N. Shigemura, Y. Yanagawa, K. Obata, H. Ueno, et al.
Discrimination of taste qualities among mouse fungiform taste bud cells
J. Physiol., September 15, 2009; 587(18): 4425 - 4439.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
Y. A. Huang, Y. Maruyama, R. Stimac, and S. D. Roper
Presynaptic (Type III) cells in mouse taste buds sense sour (acid) taste
J. Physiol., June 15, 2008; 586(12): 2903 - 2912.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
S. Kataoka, R. Yang, Y. Ishimaru, H. Matsunami, J. Sevigny, J. C. Kinnamon, and T. E. Finger
The Candidate Sour Taste Receptor, PKD2L1, Is Expressed by Type III Taste Cells in the Mouse
Chem Senses, March 1, 2008; 33(3): 243 - 254.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
V. Lyall, T.-H. T. Phan, S. Mummalaneni, M. Mansouri, G. L. Heck, G. Kobal, and J. A. DeSimone
Effect of Nicotine on Chorda Tympani Responses to Salty and Sour Stimuli
J Neurophysiol, September 1, 2007; 98(3): 1662 - 1674.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. Treesukosol, V. Lyall, G. L. Heck, J. A. DeSimone, and A. C. Spector
A psychophysical and electrophysiological analysis of salt taste in Trpv1 null mice
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1799 - R1809.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. A. DeSimone and V. Lyall
Taste Receptors in the Gastrointestinal Tract III. Salty and sour taste: sensing of sodium and protons by the tongue
Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1005 - G1010.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. M. Breza, K. S. Curtis, and R. J. Contreras
Temperature Modulates Taste Responsiveness and Stimulates Gustatory Neurons in the Rat Geniculate Ganglion
J Neurophysiol, February 1, 2006; 95(2): 674 - 685.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
V. Lyall, H. Pasley, T.-H. T. Phan, S. Mummalaneni, G. L. Heck, A. K. Vinnikova, and J. A. DeSimone
Intracellular pH Modulates Taste Receptor Cell Volume and the Phasic Part of the Chorda Tympani Response to Acids
J. Gen. Physiol., December 27, 2005; 127(1): 15 - 34.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
V. Lyall, G. L. Heck, T.-H. T. Phan, S. Mummalaneni, S. A. Malik, A. K. Vinnikova, and J. A. DeSimone
Ethanol Modulates the VR-1 Variant Amiloride-insensitive Salt Taste Receptor. I. Effect on TRC Volume and Na+ Flux
J. Gen. Physiol., May 31, 2005; 125(6): 569 - 585.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
V. Lyall, G. L. Heck, T.-H. T. Phan, S. Mummalaneni, S. A. Malik, A. K. Vinnikova, and J. A. DeSimone
Ethanol Modulates the VR-1 Variant Amiloride-insensitive Salt Taste Receptor. II. Effect on Chorda Tympani Salt Responses
J. Gen. Physiol., May 31, 2005; 125(6): 587 - 600.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
O. Lugaz, A.-M. Pillias, N. Boireau-Ducept, and A. Faurion
Time-Intensity Evaluation of Acid Taste in Subjects with Saliva High Flow and Low Flow Rates for Acids of Various Chemical Properties
Chem Senses, January 1, 2005; 30(1): 89 - 103.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. W. Putnam, J. A. Filosa, and N. A. Ritucci
Cellular mechanisms involved in CO2 and acid signaling in chemosensitive neurons
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1493 - C1526.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
W. Lin, C. A. Burks, D. R. Hansen, S. C. Kinnamon, and T. A. Gilbertson
Taste Receptor Cells Express pH-Sensitive Leak K+ Channels
J Neurophysiol, November 1, 2004; 92(5): 2909 - 2919.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
T. A. Richter, G. A. Dvoryanchikov, N. Chaudhari, and S. D. Roper
Acid-Sensitive Two-Pore Domain Potassium (K2P) Channels in Mouse Taste Buds
J Neurophysiol, September 1, 2004; 92(3): 1928 - 1936.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. A. Richter, G. A. Dvoryanchikov, S. D. Roper, and N. Chaudhari
Acid-Sensing Ion Channel-2 Is Not Necessary for Sour Taste in Mice
J. Neurosci., April 21, 2004; 24(16): 4088 - 4091.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. Lyall, R. I. Alam, S. A. Malik, T.-H. T. Phan, A. K. Vinnikova, G. L. Heck, and J. A. DeSimone
Basolateral Na+-H+ exchanger-1 in rat taste receptor cells is involved in neural adaptation to acidic stimuli
J. Physiol., April 1, 2004; 556(1): 159 - 173.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. K. Vinnikova, R. I. Alam, S. A. Malik, G. L. Ereso, G. M. Feldman, J. M. McCarty, M. A. Knepper, G. L. Heck, J. A. DeSimone, and V. Lyall
Na+-H+ Exchange Activity in Taste Receptor Cells
J Neurophysiol, March 1, 2004; 91(3): 1297 - 1313.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
O. Rossier, J. Cao, T. Huque, A. I. Spielman, R. S. Feldman, J. F. Medrano, J. G. Brand, and J. le Coutre
Analysis of a Human Fungiform Papillae cDNA Library and Identification of Taste-related Genes
Chem Senses, January 1, 2004; 29(1): 13 - 23.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
T A Richter, A Caicedo, and S D Roper
Sour Taste Stimuli Evoke Ca2+ and pH Responses in Mouse Taste Cells
J. Physiol., March 1, 2003; 547(2): 475 - 483.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
S. A. Simon
Interactions between Salt and Acid Stimuli: A Lesson in Gustation from Simultaneous Epithelial and Neural Recordings
J. Gen. Physiol., November 25, 2002; 120(6): 787 - 791.
[Full Text] [PDF]

Home page
 JGPHome page
V. Lyall, R. I. Alam, T.-H. T. Phan, O. F. Russell, S. A. Malik, G. L. Heck, and J. A. DeSimone
Modulation of Rat Chorda Tympani NaCl Responses and Intracellular Na+ Activity in Polarized Taste Receptor Cells by pH
J. Gen. Physiol., November 25, 2002; 120(6): 793 - 815.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. P. McCluskey and D. L. Hill
Sensitive periods for the effect of dietary sodium restriction on intact and denervated taste receptor cells
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2002; 283(5): R1275 - R1284.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Caicedo, K.-N. Kim, and S. D Roper
Individual mouse taste cells respond to multiple chemical stimuli
J. Physiol., October 15, 2002; 544(2): 501 - 509.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
V. Danilova, Y. Danilov, T. Roberts, J.-M. Tinti, C. Nofre, and G. Hellekant
Sense of Taste in a New World Monkey, the Common Marmoset: Recordings From the Chorda Tympani and Glossopharyngeal Nerves
J Neurophysiol, August 1, 2002; 88(2): 579 - 594.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
W. Lin, T. Ogura, and S. C. Kinnamon
Acid-Activated Cation Currents in Rat Vallate Taste Receptor Cells
J Neurophysiol, July 1, 2002; 88(1): 133 - 141.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J A Filosa, J B Dean, and R W Putnam
Role of intracellular and extracellular pH in the chemosensitive response of rat locus coeruleus neurones
J. Physiol., June 1, 2002; 541(2): 493 - 509.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
V. Lyall, R. I. Alam, T.-H. T. Phan, D. Q. Phan, G. L. Heck, and J. A. DeSimone
Excitation and Adaptation in the Detection of Hydrogen Ions by Taste Receptor Cells: A Role for cAMP and Ca2+
J Neurophysiol, January 1, 2002; 87(1): 399 - 408.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. A. DeSimone, V. Lyall, G. L. Heck, T.-H. T. Phan, R. I. Alam, G. M. Feldman, and R. M. Buch
A Novel Pharmacological Probe Links the Amiloride-Insensitive NaCl, KCl, and NH4Cl Chorda Tympani Taste Responses
J Neurophysiol, November 1, 2001; 86(5): 2638 - 2641.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online