Am J Physiol Cell Physiol AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 281: C166-C178, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (37)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parfenova, H.
Right arrow Articles by Leffler, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parfenova, H.
Right arrow Articles by Leffler, C. W.
Vol. 281, Issue 1, C166-C178, July 2001

Dynamics of nuclear localization sites for COX-2 in vascular endothelial cells

Helena Parfenova1, Vladimir N. Parfenov3, Boris V. Shlopov2, Vladimir Levine1, Sheryl Falkos1, Massroor Pourcyrous1, and Charles W. Leffler1

1 Departments of Physiology, Pediatrics, and Obstetrics & Gynecology and 2 Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, Tennessee 38163; and 3 Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia 194064

We investigated the relationships among expression, activity, and spatial organization of cyclooxygenase (COX-1 and COX-2) in endothelial cells from porcine and human cerebral microvessels and from human umbilical vein. In quiescent cells, COX-1 was detected in the perinuclear zone and the cytoplasm, while COX-2 was mainly a nuclear resident possibly connected with the nuclear matrix. COX-2 immunogold labeling was situated in the nuclear envelope, at the nuclear pores, and in connection with the perichromatin regions of the nucleus, considered to be the sites of active transcription. In human endothelial cells transcriptionally activated by interleukin (IL)-1beta , the nucleus remained a major COX-2 localization site during the first 12 h of stimulation, when COX-2 expression was maximally induced. The continuous rise in prostanoid synthesis at 17-23 h of stimulation was associated with COX-2 relocation from the nucleus to the nuclear envelope and the cytoplasm. IL-1beta did not affect COX-1 expression, activity, and localization. COX-2 nuclear localization sites and trafficking between the nucleus and the cytoplasm in endothelial cells may indicate a novel function of COX-2 in regulating gene expression.

prostanoids; endothelial cell; interleukin-1beta ; nucleus


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
B. Garcia-Bueno, J. Serrats, and P. E. Sawchenko
Cerebrovascular Cyclooxygenase-1 Expression, Regulation, and Role in Hypothalamic-Pituitary-Adrenal Axis Activation by Inflammatory Stimuli
J. Neurosci., October 14, 2009; 29(41): 12970 - 12981.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Li, L. Kuo, and J. N. Stallone
Estrogen potentiates constrictor prostanoid function in female rat aorta by upregulation of cyclooxygenase-2 and thromboxane pathway expression
Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2444 - H2455.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Vazquez-Tello, L. Fan, X. Hou, J.-S. Joyal, J. A. Mancini, C. Quiniou, R. I. Clyman, F. Gobeil Jr., D. R. Varma, and S. Chemtob
Intracellular-specific colocalization of prostaglandin E2 synthases and cyclooxygenases in the brain
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1155 - R1163.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. A. Yakubu and C. W. Leffler
L-type voltage-dependent Ca2+ channels in cerebral microvascular endothelial cells and ET-1 biosynthesis
Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1687 - C1695.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Parfenova, R. A. Neff III, J. S. Alonso, B. V. Shlopov, C. N. Jamal, S. A. Sarkisova, and C. W. Leffler
Cerebral vascular endothelial heme oxygenase: expression, localization, and activation by glutamate
Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1954 - C1963.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online