| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
channels in
rabbit gastric mucosa
Departments of 1 Molecular and Cellular Physiology and 2 Cell Biology, Neurobiology, and Anatomy, University of Cincinnati College of Medicine, Cincinnati, Ohio 43267-0576
HCl secretion across the parietal cell apical secretory
membrane involves the H+-K+-ATPase, the ClC-2
Cl
channel, and a K+ channel. In the present
study, the cellular and subcellular distribution of ClC-2 mRNA and
protein was determined in the rabbit gastric mucosa and in isolated
gastric glands. ClC-2 mRNA was localized to parietal cells by in situ
hybridization and by direct in situ RT-PCR. By immunoperoxidase
microscopy, ClC-2 protein was concentrated in parietal cells.
Immunofluorescent confocal microscopy suggested that the ClC-2 was
localized to the secretory canalicular membrane of stimulated parietal
cells and to intracellular structures of resting parietal cells.
Immunogold electron microscopy confirmed that ClC-2 is in the secretory
canalicular membrane of stimulated cells and in tubulovesicles of
resting parietal cells. These findings, together with previous
functional characterization of the native and recombinant channel,
strongly indicate that ClC-2 is the Cl channel, which
together with the H+-K+-ATPase and a
K+ channel, results in HCl secretion across the parietal
cell secretory membrane.
gastric chloride channel; hydrochloric acid secretion; secretory canalicular membrane; tubulovesicles
This article has been cited by other articles:
|
|
 |
|
  V. G. Romanenko, T. Nakamoto, M. A. Catalan, M. Gonzalez-Begne, G. J. Schwartz, Y. Jaramillo, F. V. Sepulveda, C. D. Figueroa, and J. E. Melvin Clcn2 encodes the hyperpolarization-activated chloride channel in the ducts of mouse salivary glands Am J Physiol Gastrointest Liver Physiol, November 1, 2008; 295(5): G1058 - G1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Perez-Zoghbi, A. Mayora, M. C. Ruiz, and F. Michelangeli Heterogeneity of acid secretion induced by carbachol and histamine along the gastric gland axis and its relationship to [Ca2+]i Am J Physiol Gastrointest Liver Physiol, October 1, 2008; 295(4): G671 - G681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. F. Bao, L. Liu, J. Self, B. J. Duke, R. Ueno, and D. C. Eaton A synthetic prostone activates apical chloride channels in A6 epithelial cells Am J Physiol Gastrointest Liver Physiol, August 1, 2008; 295(2): G234 - G251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zavros, M. A. Orr, C. Xiao, and D. H. Malinowska Sonic hedgehog is associated with H+-K+-ATPase-containing membranes in gastric parietal cells and secreted with histamine stimulation Am J Physiol Gastrointest Liver Physiol, July 1, 2008; 295(1): G99 - G111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Lapierre, K. M. Avant, C. M. Caldwell, A.-J. L. Ham, S. Hill, J. A. Williams, A. J. Smolka, and J. R. Goldenring Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1249 - G1262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Camilleri, A. E. Bharucha, R. Ueno, D. Burton, G. M. Thomforde, K. Baxter, S. McKinzie, and A. R. Zinsmeister Effect of a selective chloride channel activator, lubiprostone, on gastrointestinal transit, gastric sensory, and motor functions in healthy volunteers Am J Physiol Gastrointest Liver Physiol, May 1, 2006; 290(5): G942 - G947. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cuppoletti, D. H. Malinowska, K. P. Tewari, Q.-j. Li, A. M. Sherry, M. L. Patchen, and R. Ueno SPI-0211 activates T84 cell chloride transport and recombinant human ClC-2 chloride currents Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1173 - C1183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Malinowska, A. M. Sherry, K. P. Tewari, and J. Cuppoletti Gastric parietal cell secretory membrane contains PKA- and acid-activated Kir2.1 K+ channels Am J Physiol Cell Physiol, March 1, 2004; 286(3): C495 - C506. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. Varela, M. I. Niemeyer, L P. Cid, and F. V Sepulveda Effect of an N-terminus deletion on voltage-dependent gating of the ClC-2 chloride channel J. Physiol., October 15, 2002; 544(2): 363 - 372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Catalan, I. Cornejo, C. D. Figueroa, M. I. Niemeyer, F. V. Sepulveda, and L. P. Cid ClC-2 in guinea pig colon: mRNA, immunolabeling, and functional evidence for surface epithelium localization Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G1004 - G1013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. H Hirst K+ recycling and gastric acid secretion J. Physiol., April 1, 2002; 540(1): 1 - 1. [Full Text] [PDF]
|
|
|
|
|
|
J. Lipecka, M. Bali, A. Thomas, P. Fanen, A. Edelman, and J. Fritsch Distribution of ClC-2 chloride channel in rat and human epithelial tissues Am J Physiol Cell Physiol, April 1, 2002; 282(4): C805 - C816. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
