| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Institut National de la Santé et de la Recherche Médicale U. 467, Faculté de Médecine Necker 75730 Paris Cedex 15; and 2 Centre National de la Recherche Scientifique Unité Propre de Recherche 1524, Hôpital St-Vincent de Paul 75674 Paris, France
The almost ubiquitously expressed ClC-2 chloride channel is
activated by hyperpolarization and osmotic cell swelling. Osmotic swelling also activates a different class of outwardly rectifying chloride channels, and several reports point to a link between protein
tyrosine phosphorylation and activation of these channels. This study
examines the possibility that transforming growth factor-
(TGF-)
modulates ClC-2 activity in human colonic epithelial (T84) cells.
TGF- (0.17 nM) irreversibly inhibited ClC-2 current in nystatin-perforated whole cell patch-clamp experiments, whereas a
superimposed reversible activation of the current was observed at 8.3 nM TGF-. Both effects required activation of the intrinsic epidermal
growth factor receptor (EGFR) tyrosine kinase activity, of
phosphoinositide 3-kinase, and of protein kinase C. With
microspectrofluorimetry of the pH-sensitive fluorescent dye
2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, TGF-
was shown to reversibly alkalinize T84 cells at 8.3 nM but not at 0.17 nM, suggesting that 8.3 nM TGF--induced alkalinization activates
ClC-2 current. This study indicates that ClC-2 channels are
targets for EGFR signaling in epithelial cells.
hydrogen ion concentration; protein kinase C; signal transduction; patch-clamp techniques
This article has been cited by other articles:
|
|
 |
|
  H. F. Bao, L. Liu, J. Self, B. J. Duke, R. Ueno, and D. C. Eaton A synthetic prostone activates apical chloride channels in A6 epithelial cells Am J Physiol Gastrointest Liver Physiol, August 1, 2008; 295(2): G234 - G251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. O'Mahony, F. Toumi, M. S. Mroz, G. Ferguson, and S. J. Keely Induction of Na+/K+/2Cl- cotransporter expression mediates chronic potentiation of intestinal epithelial Cl- secretion by EGF Am J Physiol Cell Physiol, June 1, 2008; 294(6): C1362 - C1370. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Hegab, T. Sakamoto, A. Nomura, Y. Ishii, Y. Morishima, T. Iizuka, T. Kiwamoto, Y. Matsuno, S. Homma, and K. Sekizawa Niflumic Acid and AG-1478 Reduce Cigarette Smoke-Induced Mucin Synthesis: The Role of hCLCA1 Chest, April 1, 2007; 131(4): 1149 - 1156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Moeser, P. K. Nighot, K. J. Engelke, R. Ueno, and A. T. Blikslager Recovery of mucosal barrier function in ischemic porcine ileum and colon is stimulated by a novel agonist of the ClC-2 chloride channel, lubiprostone Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G647 - G656. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cheng, M.-J. Kim, G. Jia, and D. K. Agrawal Involvement of chloride channels in IGF-I-induced proliferation of porcine arterial smooth muscle cells Cardiovasc Res, January 1, 2007; 73(1): 198 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Cid, M. I. Niemeyer, and F. V. Sepulveda ClC-2 channels get new partners. Focus on "Association between Hsp90 and the ClC-2 chloride channel upregulates channel function" Am J Physiol Cell Physiol, January 1, 2006; 290(1): C42 - C44. [Full Text] [PDF]
|
|
|
|
|
|
A. Hinzpeter, J. Lipecka, F. Brouillard, M. Baudoin-Legros, M. Dadlez, A. Edelman, and J. Fritsch Association between Hsp90 and the ClC-2 chloride channel upregulates channel function Am J Physiol Cell Physiol, January 1, 2006; 290(1): C45 - C56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cuppoletti, D. H. Malinowska, K. P. Tewari, Q.-j. Li, A. M. Sherry, M. L. Patchen, and R. Ueno SPI-0211 activates T84 cell chloride transport and recombinant human ClC-2 chloride currents Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1173 - C1183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. U. Dhani, R. Mohammad-Panah, N. Ahmed, C. Ackerley, M. Ramjeesingh, and C. E. Bear Evidence for a Functional Interaction between the ClC-2 Chloride Channel and the Retrograde Motor Dynein Complex J. Biol. Chem., April 25, 2003; 278(18): 16262 - 16270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Howe, J. Gauldie, and D. M. McKay TGF-beta effects on epithelial ion transport and barrier: reduced Cl- secretion blocked by a p38 MAPK inhibitor Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1667 - C1674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lipecka, M. Bali, A. Thomas, P. Fanen, A. Edelman, and J. Fritsch Distribution of ClC-2 chloride channel in rat and human epithelial tissues Am J Physiol Cell Physiol, April 1, 2002; 282(4): C805 - C816. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
