Am J Physiol Cell Physiol AJP: Heart and Circulatory Physiology
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Am J Physiol Cell Physiol 280: C1555-C1560, 2001;
0363-6143/01 $5.00
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Vol. 280, Issue 6, C1555-C1560, June 2001

Effect of cyclic stretch on alpha -subunit mRNA expression of Na+-K+-ATPase in aortic smooth muscle cells

Nancy Sevieux1, Jawed Alam2, and Emel Songu-Mize1

1 Department of Pharmacology and Experimental Therapeutics, Louisiana State University Health Sciences Center, and 2 Department of Molecular Genetics, Ochsner Medical Foundation, New Orleans, Louisiana 70112

We previously demonstrated that protein expression of both alpha 1- and alpha 2-catalytic subunits of the Na+-K+-ATPase is elevated after a 2- to 4-day chronic cyclic stretch regimen in cultured aortic smooth muscle cells (ASMC). In the present study, we investigated whether cyclic stretch affects mRNA expression of the alpha -isoforms of the Na+-K+-ATPase. Using a stretch apparatus, rat ASMC were cyclically stretched 10 or 20% of their length for 1, 3, or 6 h. alpha -Isoform mRNA levels were measured using Northern analysis. A 3-h 10% stretch had no significant affect on mRNA expression for either isoform, but a 20% stretch increased mRNA of both isoforms approximately twofold. Whereas a 6-h 20% stretch increased alpha 1 mRNA by 3.3-fold, alpha 2 was not affected any further. Actinomycin D blocked the stretch-induced stimulation of mRNA expression of both alpha -subunits. In conclusion, cyclic stretch stimulates the mRNA expression of both alpha 1- and alpha 2-subunits of Na+-K+-ATPase. The sensitivity of the two genes to the degree and duration of stretch is different. The stretch-induced increase of mRNA may be a result of increased transcription.

sodium pump; transcription


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