Am J Physiol Cell Physiol Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 280: C1340-C1348, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sedensky, M. M.
Right arrow Articles by Morgan, P. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sedensky, M. M.
Right arrow Articles by Morgan, P. G.
Vol. 280, Issue 5, C1340-C1348, May 2001

SPECIAL TOPIC
Model Organisms: New Insights Into Ion Channel and Transporter Function. Stomatin homologues interact in Caenorhabditis elegans

M. M. Sedensky, J. M. Siefker, and P. G. Morgan

Departments of Anesthesiology and Genetics, University Hospitals and Case Western Reserve University, Cleveland, Ohio 44106

In C. elegans the protein UNC-1 is a major determinant of anesthetic sensitivity and is a close homologue of the mammalian protein stomatin. In humans stomatin is missing from erythrocyte membranes in the hemolytic disease overhydrated hereditary stomatocytosis, despite an apparently normal stomatin gene. Overhydrated hereditary stomatocytosis is characterized by alteration of the normal transmembrane gradients of sodium and potassium. Stomatin has been shown to interact genetically with sodium channels. It is also postulated that stomatin is important in the organization of lipid rafts. We demonstrate here that antibodies against UNC-1 stain the major nerve tracts of Caenorhabditis elegans, with very intense staining of the nerve ring. We also found that a gene encoding a stomatin-like protein, UNC-24, affects anesthetic sensitivity and is genetically epistatic to unc-1. In the absence of UNC-24, the staining of the nerve ring by anti-UNC-1 is abolished, despite normal transcriptional levels of the unc-1 mRNA. Western blots indicate that UNC-24 probably affects the stability of the UNC-1 protein. UNC-24 may therefore be necessary for the correct placement of UNC-1 in the cell membrane and organization of lipid rafts.

lipid rafts; anesthetics; nematodes; development; genetics


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Mairhofer, M. Steiner, U. Salzer, and R. Prohaska
Stomatin-like Protein-1 Interacts with Stomatin and Is Targeted to Late Endosomes
J. Biol. Chem., October 16, 2009; 284(42): 29218 - 29229.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
K. Hattori, M. Inoue, T. Inoue, H. Arai, and H.-o. Tamura
A Novel Sulfotransferase Abundantly Expressed in the Dauer Larvae of Caenorhabditis elegans.
J. Biochem., March 1, 2006; 139(3): 355 - 362.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. Syntichaki and N. Tavernarakis
Genetic Models of Mechanotransduction: The Nematode Caenorhabditis elegans
Physiol Rev, October 1, 2004; 84(4): 1097 - 1153.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. M. Sedensky, J. M. Siefker, J. Y. Koh, D. M. Miller III, and P. G. Morgan
A stomatin and a degenerin interact in lipid rafts of the nervous system of Caenorhabditis elegans
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C468 - C474.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. L. Hiller, T. Akompong, J. S. Morrow, A. A. Holder, and K. Haldar
Identification of a Stomatin Orthologue in Vacuoles Induced in Human Erythrocytes by Malaria Parasites: A ROLE FOR MICROBIAL RAFT PROTEINS IN APICOMPLEXAN VACUOLE BIOGENESIS
J. Biol. Chem., November 28, 2003; 278(48): 48413 - 48421.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Rubin and F. Ismail-Beigi
Distribution of Glut1 in detergent-resistant membranes (DRMs) and non-DRM domains: effect of treatment with azide
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C377 - C383.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Kobayakawa, R. Hayashi, K. Morita, K. Miyamichi, Y. Oka, A. Tsuboi, and H. Sakano
Stomatin-Related Olfactory Protein, SRO, Specifically Expressed in the Murine Olfactory Sensory Neurons
J. Neurosci., July 15, 2002; 22(14): 5931 - 5937.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
K. W. Miller
The nature of sites of general anaesthetic action
Br. J. Anaesth., July 1, 2002; 89(1): 17 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Br J AnaesthHome page
H. A. Nash
In vivo genetics of anaesthetic action
Br. J. Anaesth., July 1, 2002; 89(1): 143 - 155.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. A. Humphrey, M. M. Sedensky, and P. G. Morgan
Understanding anesthesia: making genetic sense of the absence of senses
Hum. Mol. Genet., May 15, 2002; 11(10): 1241 - 1249.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. P. Winn
Not All in the Family: Mutations of Podocin in Sporadic Steroid-Resistant Nephrotic Syndrome
J. Am. Soc. Nephrol., February 1, 2002; 13(2): 577 - 579.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online