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1 Program in Cellular and Molecular Physiology, 3 Program in Neuroscience, and 2 Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655
We examined the effects of arachidonic acid
(AA) on whole cell Ca2+ channel activity in rat superior
cervical ganglion neurons. Our companion paper (Liu L, Barrett CF, and
Rittenhouse AR. Am J Physiol Cell Physiol 280:
C1293-C1305, 2001) demonstrates that AA induces several
effects, including enhancement of current amplitude at negative
voltages, and increased activation kinetics. This study examines the
mechanisms underlying these effects. First, enhancement is rapidly
reversible by bath application of BSA. Second, enhancement appears to
occur extracellularly, since intracellular albumin was without effect
on enhancement, and bath-applied arachidonoyl coenzyme A, an
amphiphilic AA analog that cannot cross the cell membrane, mimicked
enhancement. In addition, enhancement is voltage dependent, in that
currents were enhanced to the greatest degree at 
10 mV, whereas
virtually no enhancement occurred positive of +30 mV. We also
demonstrate that AA-induced increases in activation kinetics are
correlated with enhancement of current amplitude. An observed increase
in the voltage sensitivity may underlie these effects. Finally, the
majority of enhancement is mediated through N-type current, thus
providing the first demonstration that this current type can be
enhanced by AA.
calcium channel; eicosatetraynoic acid; fatty acids; arachidonoyl coenzyme A; voltage dependence
This article has been cited by other articles:
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  M. L. Roberts-Crowley and A. R. Rittenhouse Arachidonic acid inhibition of L-type calcium (CaV1.3b) channels varies with accessory CaV{beta} subunits J. Gen. Physiol., April 1, 2009; 133(4): 387 - 403. [Abstract] [Full Text] [PDF]
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 J. Guo, D. J. Williams, and S. R. Ikeda N-Arachidonoyl L-Serine, a Putative Endocannabinoid, Alters the Activation of N-Type Ca2+ Channels in Sympathetic Neurons J Neurophysiol, August 1, 2008; 100(2): 1147 - 1151. [Abstract] [Full Text] [PDF]
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 L. Liu, J. F. Heneghan, T. Mitra-Ganguli, M. L. Roberts-Crowley, and A. R. Rittenhouse Role of PIP2 in regulating versus modulating Ca2+ channel activity J. Physiol., September 15, 2007; 583(3): 1165 - 1166. [Full Text] [PDF]
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A. Fisyunov, V. Tsintsadze, R. Min, N. Burnashev, and N. Lozovaya Cannabinoids Modulate the P-Type High-Voltage-Activated Calcium Currents in Purkinje Neurons J Neurophysiol, September 1, 2006; 96(3): 1267 - 1277. [Abstract] [Full Text] [PDF]
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Y. Lin, S. I. McDonough, and D. Lipscombe Alternative Splicing in the Voltage-Sensing Region of N-Type CaV2.2 Channels Modulates Channel Kinetics J Neurophysiol, November 1, 2004; 92(5): 2820 - 2830. [Abstract] [Full Text] [PDF]
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K. Talavera, M. Staes, A. Janssens, G. Droogmans, and B. Nilius Mechanism of Arachidonic Acid Modulation of the T-type Ca2+ Channel {alpha}1G J. Gen. Physiol., August 30, 2004; 124(3): 225 - 238. [Abstract] [Full Text] [PDF]
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 L. Liu, C. F. Barrett, and A. R. Rittenhouse Arachidonic acid both inhibits and enhances whole cell calcium currents in rat sympathetic neurons Am J Physiol Cell Physiol, May 1, 2001; 280(5): C1293 - C1305. [Abstract] [Full Text] [PDF]
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