Am J Physiol Cell Physiol AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 280: C1184-C1192, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (80)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walker, R. L.
Right arrow Articles by Horowitz, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walker, R. L.
Right arrow Articles by Horowitz, B.
Vol. 280, Issue 5, C1184-C1192, May 2001

Differential expression and alternative splicing of TRP channel genes in smooth muscles

Rebecca L. Walker, Joseph R. Hume, and Burton Horowitz

Department of Physiology, University of Nevada School of Medicine, Reno, Nevada 89557

Nonselective cation channels (NSCC) are targets of excitatory agonists in smooth muscle, representing the nonselective cation current Icat. Na+ influx through NSCC causes depolarizations and activates voltage-dependent Ca2+ channels, resulting in contraction. The molecular identity of Icat in smooth muscle has not been elucidated; however, products of the transient receptor potential (TRP) genes have characteristics similar to native Icat. We have determined the levels of TRP transcriptional expression in several murine and canine gastrointestinal and vascular smooth muscles and have analyzed the alternative processing of these transcripts. Of the seven TRP gene family members, transcripts for TRP4, TRP6, and TRP7 were detected in all murine and canine smooth muscle cell preparations. TRP3 was detected only in canine renal artery smooth muscle cells. The full-length cDNAs for TRP4, TRP6, and TRP7, as well as one splice variant of TRP4 and two splice variants of TRP7, were cloned from murine colonic smooth muscle. Quantitative RT-PCR determined the relative amounts of TRP4, TRP6, and TRP7 transcripts, as well as that of the splice variants, in several murine smooth muscles. TRP4 is the most highly expressed, while TRP6 and TRP7 are expressed at a lower level in the same tissues. Splice variants for TRP7, deleted for exons encoding amino acids including transmembrane segment S1, predominated in murine smooth muscles, while the full-length form of the transcript was expressed in canine smooth muscles.

calcium channels; gastrointestinal; vascular; ribonucleic acid expression


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
S. Sours-Brothers, M. Ding, S. Graham, and R. Ma
Interaction Between TRPC1/TRPC4 Assembly and STIM1 Contributes to Store-Operated Ca2+ Entry in Mesangial Cells
Experimental Biology and Medicine, June 1, 2009; 234(6): 673 - 682.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. C. Ng, M. D. McCormack, J. A. Airey, C. A. Singer, P. S. Keller, X.-M. Shen, and J. R. Hume
TRPC1 and STIM1 mediate capacitative Ca2+ entry in mouse pulmonary arterial smooth muscle cells
J. Physiol., June 1, 2009; 587(11): 2429 - 2442.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. Yogi, G. E. Callera, R. Tostes, and R. M. Touyz
Bradykinin regulates calpain and proinflammatory signaling through TRPM7-sensitive pathways in vascular smooth muscle cells
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2009; 296(2): R201 - R207.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
W. Lu, J. Wang, L. A. Shimoda, and J. T. Sylvester
Differences in STIM1 and TRPC expression in proximal and distal pulmonary arterial smooth muscle are associated with differences in Ca2+ responses to hypoxia
Am J Physiol Lung Cell Mol Physiol, July 1, 2008; 295(1): L104 - L113.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. R. Kovac, T. Chrones, and S. M. Sims
Temporal and spatial dynamics underlying capacitative calcium entry in human colonic smooth muscle
Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G88 - G98.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. C. Ng, B. D. Kyle, A. R. Lennox, X.-M. Shen, W. J. Hatton, and J. R. Hume
Cell culture alters Ca2+ entry pathways activated by store-depletion or hypoxia in canine pulmonary arterial smooth muscle cells
Am J Physiol Cell Physiol, January 1, 2008; 294(1): C313 - C323.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. K. Fellner and W. J. Arendshorst
Angiotensin II-stimulated Ca2+ entry mechanisms in afferent arterioles: role of transient receptor potential canonical channels and reverse Na+/Ca2+ exchange
Am J Physiol Renal Physiol, January 1, 2008; 294(1): F212 - F219.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
T. Sakamoto, T. Unno, T. Kitazawa, T. Taneike, M. Yamada, J. Wess, M. Nishimura, and S. Komori
Three distinct muscarinic signalling pathways for cationic channel activation in mouse gut smooth muscle cells
J. Physiol., July 1, 2007; 582(1): 41 - 61.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. Inoue, L. J. Jensen, J. Shi, H. Morita, M. Nishida, A. Honda, and Y. Ito
Transient Receptor Potential Channels in Cardiovascular Function and Disease
Circ. Res., July 21, 2006; 99(2): 119 - 131.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Sours, J. Du, S. Chu, M. Ding, X. J. Zhou, and R. Ma
Expression of canonical transient receptor potential (TRPC) proteins in human glomerular mesangial cells
Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1507 - F1515.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. T. Kim, B. J. Kim, J. H. Lee, S. C. Kwon, D. S. Yeon, D. K. Yang, I. So, and K. W. Kim
Involvement of calmodulin and myosin light chain kinase in activation of mTRPC5 expressed in HEK cells
Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1031 - C1040.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. L. Jernigan, B. R. S. Broughton, B. R. Walker, and T. C. Resta
Impaired NO-dependent inhibition of store- and receptor-operated calcium entry in pulmonary vascular smooth muscle after chronic hypoxia
Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L517 - L525.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
R. Ma, J. Du, S. Sours, and M. Ding
Store-Operated Ca2+ Channel in Renal Microcirculation and Glomeruli
Experimental Biology and Medicine, February 1, 2006; 231(2): 145 - 153.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
B. M. Sanborn, C.-Y. Ku, S. Shlykov, and L. Babich
Molecular Signaling Through G-Protein-Coupled Receptors and the Control of Intracellular Calcium in Myometrium
Reproductive Sciences, October 1, 2005; 12(7): 479 - 487.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. A. Reading, S. Earley, B. J. Waldron, D. G. Welsh, and J. E. Brayden
TRPC3 mediates pyrimidine receptor-induced depolarization of cerebral arteries
Am J Physiol Heart Circ Physiol, May 1, 2005; 288(5): H2055 - H2061.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. C. Ng, S. M Wilson, and J. R Hume
Mobilization of sarcoplasmic reticulum stores by hypoxia leads to consequent activation of capacitative Ca2+ entry in isolated canine pulmonary arterial smooth muscle cells
J. Physiol., March 1, 2005; 563(2): 409 - 419.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. Kunichika, J. W. Landsberg, Y. Yu, H. Kunichika, P. A. Thistlethwaite, L. J. Rubin, and J. X.-J. Yuan
Bosentan Inhibits Transient Receptor Potential Channel Expression in Pulmonary Vascular Myocytes
Am. J. Respir. Crit. Care Med., November 15, 2004; 170(10): 1101 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. Kunichika, Y. Yu, C. V. Remillard, O. Platoshyn, S. Zhang, and J. X.-J. Yuan
Overexpression of TRPC1 enhances pulmonary vasoconstriction induced by capacitative Ca2+ entry
Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L962 - L969.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. J. Beech, K. Muraki, and R. Flemming
Non-selective cationic channels of smooth muscle and the mammalian homologues of Drosophila TRP
J. Physiol., September 15, 2004; 559(3): 685 - 706.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M.-J. Lin, G. P.H. Leung, W.-M. Zhang, X.-R. Yang, K.-P. Yip, C.-M. Tse, and J. S.K. Sham
Chronic Hypoxia-Induced Upregulation of Store-Operated and Receptor-Operated Ca2+ Channels in Pulmonary Arterial Smooth Muscle Cells: A Novel Mechanism of Hypoxic Pulmonary Hypertension
Circ. Res., September 3, 2004; 95(5): 496 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
X. Wang, J. L. Pluznick, P. Wei, B. J. Padanilam, and S. C. Sansom
TRPC4 forms store-operated Ca2+ channels in mouse mesangial cells
Am J Physiol Cell Physiol, August 1, 2004; 287(2): C357 - C364.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Wang, L. A. Shimoda, and J. T. Sylvester
Capacitative calcium entry and TRPC channel proteins are expressed in rat distal pulmonary arterial smooth muscle
Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L848 - L858.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. G. Babich, C.-Y. Ku, H. W.J. Young, H. Huang, M. R. Blackburn, and B. M. Sanborn
Expression of Capacitative Calcium TrpC Proteins in Rat MyometriumDuring Pregnancy
Biol Reprod, April 1, 2004; 70(4): 919 - 924.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
C. S. Facemire, P. J. Mohler, and W. J. Arendshorst
Expression and relative abundance of short transient receptor potential channels in the rat renal microcirculation
Am J Physiol Renal Physiol, March 1, 2004; 286(3): F546 - F551.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
S. G. Shlykov, M. Yang, J. L. Alcorn, and B. M. Sanborn
Capacitative Cation Entry in Human Myometrial Cells and Augmentation by hTrpC3 Overexpression
Biol Reprod, August 1, 2003; 69(2): 647 - 655.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Huang, Z. Xing, Z. Luan, T. Wu, X. Wu, and G. Hu
A Specific Splicing Variant of SVH, a Novel Human Armadillo Repeat Protein, Is Up-Regulated in Hepatocellular Carcinomas
Cancer Res., July 1, 2003; 63(13): 3775 - 3782.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Wang, L. G. Laurier, S. M. Sims, and H. G. Preiksaitis
Enhanced capacitative calcium entry and TRPC channel gene expression in human LES smooth muscle
Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G1074 - G1083.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Yu, M. Sweeney, S. Zhang, O. Platoshyn, J. Landsberg, A. Rothman, and J. X.-J. Yuan
PDGF stimulates pulmonary vascular smooth muscle cell proliferation by upregulating TRPC6 expression
Am J Physiol Cell Physiol, February 1, 2003; 284(2): C316 - C330.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. Ohya and B. Horowitz
Differential transcriptional expression of Ca2+ BP superfamilies in murine gastrointestinal smooth muscles
Am J Physiol Gastrointest Liver Physiol, December 1, 2002; 283(6): G1290 - G1297.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. L. Walker, S. D. Koh, G. P. Sergeant, K. M. Sanders, and B. Horowitz
TRPC4 currents have properties similar to the pacemaker current in interstitial cells of Cajal
Am J Physiol Cell Physiol, December 1, 2002; 283(6): C1637 - C1645.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. C Amberg, S. D. Koh, W. J Hatton, K. J Murray, K. Monaghan, B. Horowitz, and K. M Sanders
Contribution of Kv4 channels toward the A-type potassium current in murine colonic myocytes
J. Physiol., October 15, 2002; 544(2): 403 - 415.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. C Amberg, S. A Baker, S. D. Koh, W. J Hatton, K. J Murray, B. Horowitz, and K. M Sanders
Characterization of the A-type potassium current in murine gastric antrum
J. Physiol., October 15, 2002; 544(2): 417 - 428.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. M Wilson, H. S Mason, G. D Smith, N. Nicholson, L. Johnston, R. Janiak, and J. R Hume
Comparative capacitative calcium entry mechanisms in canine pulmonary and renal arterial smooth muscle cells
J. Physiol., September 15, 2002; 543(3): 917 - 931.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. H. Shalinsky, J. Magistretti, L. Ma, and A. A. Alonso
Muscarinic Activation of a Cation Current and Associated Current Noise in Entorhinal-Cortex Layer-II Neurons
J Neurophysiol, September 1, 2002; 88(3): 1197 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Yang, A. Gupta, S. G. Shlykov, R. Corrigan, S. Tsujimoto, and B. M. Sanborn
Multiple Trp Isoforms Implicated in Capacitative Calcium Entry Are Expressed in Human Pregnant Myometrium and Myometrial Cells
Biol Reprod, September 1, 2002; 67(3): 988 - 994.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Sweeney, Y. Yu, O. Platoshyn, S. Zhang, S. S. McDaniel, and J. X.-J. Yuan
Inhibition of endogenous TRP1 decreases capacitative Ca2+ entry and attenuates pulmonary artery smooth muscle cell proliferation
Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L144 - L155.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
C.-H. Lee, R. Rahimian, T. Szado, J. Sandhu, D. Poburko, T. Behra, L. Chan, and C. van Breemen
Sequential opening of IP3-sensitive Ca2+ channels and SOC during alpha -adrenergic activation of rabbit vena cava
Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1768 - H1777.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online