Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 280: C823-C835, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (54)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liu, J.
Right arrow Articles by Lisanti, M. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liu, J.
Right arrow Articles by Lisanti, M. P.
Vol. 280, Issue 4, C823-C835, April 2001

Caveolin-1 expression sensitizes fibroblastic and epithelial cells to apoptotic stimulation

Jun Liu1, Peiyee Lee2, Ferruccio Galbiati1, Richard N. Kitsis2,3, and Michael P. Lisanti1,3

Departments of 1 Molecular Pharmacology and 2 Medicine and Cell Biology and 3 Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, New York 10461

The potential role of caveolin-1 in apoptosis remains controversial. Here, we investigate whether caveolin-1 expression is proapoptotic or antiapoptotic using a well-defined antisense approach. We show that NIH/3T3 cells harboring antisense caveolin-1 are resistant to staurosporine-induced apoptosis, as assessed using cell morphology, DNA content, caspase 3 activation, and focal adhesion kinase cleavage. Importantly, sensitivity to apoptosis is recovered when caveolin-1 levels are restored. Conversely, recombinant stable expression of caveolin-1 in T24 bladder carcinoma cells sensitizes these cells to caspase 3 activation. Consistent with the observations using NIH/3T3 cells, downregulation of caveolin-1 in T24 cells substantially diminishes caspase 3-like activity. Loss of sensitivity to apoptotic stimulation is recovered by inhibition of the phosphatidylinositol 3-kinase pathway using LY-294002, suggesting a possible mechanism for the sensitizing effect of caveolin-1. Thus our results suggest that caveolin-1 may act as a coupling or sensitizing factor in signaling apoptotic cell death in both fibroblastic (NIH/3T3) and epithelial (T24) cells.

caveolae; caveolin; signaling


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
S. E. Park, S. H. Jeong, S.-B. Yee, T. H. Kim, Y. H. Soung, N. C. Ha, N. D. Kim, J.-Y. Park, H. R. Bae, B. S. Park, et al.
Interactions of acetylcholinesterase with caveolin-1 and subsequently with cytochrome c are required for apoptosome formation
Carcinogenesis, April 1, 2008; 29(4): 729 - 737.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
D. Volonte, C. F. McTiernan, M. Drab, M. Kasper, and F. Galbiati
Caveolin-1 and caveolin-3 form heterooligomeric complexes in atrial cardiac myocytes that are required for doxorubicin-induced apoptosis
Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H392 - H401.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
V. A. Torres, J. C. Tapia, D. A. Rodriguez, A. Lladser, C. Arredondo, L. Leyton, and A. F. G. Quest
E-Cadherin Is Required for Caveolin-1-Mediated Down-Regulation of the Inhibitor of Apoptosis Protein Survivin via Reduced {beta}-Catenin-Tcf/Lef-Dependent Transcription
Mol. Cell. Biol., November 1, 2007; 27(21): 7703 - 7717.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J.-F. Jasmin, S. Malhotra, M. Singh Dhallu, I. Mercier, D. M. Rosenbaum, and M. P. Lisanti
Caveolin-1 Deficiency Increases Cerebral Ischemic Injury
Circ. Res., March 16, 2007; 100(5): 721 - 729.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. A. Torres, J. C. Tapia, D. A. Rodriguez, M. Parraga, P. Lisboa, M. Montoya, L. Leyton, and A. F. G. Quest
Caveolin-1 controls cell proliferation and cell death by suppressing expression of the inhibitor of apoptosis protein survivin
J. Cell Sci., May 1, 2006; 119(9): 1812 - 1823.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. M. Williams and M. P. Lisanti
Caveolin-1 in oncogenic transformation, cancer, and metastasis
Am J Physiol Cell Physiol, March 1, 2005; 288(3): C494 - C506.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Li, C. H. Ren, S. A. Tahir, C. Ren, and T. C. Thompson
Caveolin-1 Maintains Activated Akt in Prostate Cancer Cells through Scaffolding Domain Binding Site Interactions with and Inhibition of Serine/Threonine Protein Phosphatases PP1 and PP2A
Mol. Cell. Biol., December 15, 2003; 23(24): 9389 - 9404.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
P. Gargalovic and L. Dory
Cellular apoptosis is associated with increased caveolin-1 expression in macrophages
J. Lipid Res., September 1, 2003; 44(9): 1622 - 1632.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
B. Razani, S. E. Woodman, and M. P. Lisanti
Caveolae: From Cell Biology to Animal Physiology
Pharmacol. Rev., September 1, 2002; 54(3): 431 - 467.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. Volonte, K. Zhang, M. P. Lisanti, and F. Galbiati
Expression of Caveolin-1 Induces Premature Cellular Senescence in Primary Cultures of Murine Fibroblasts. Stress-Induced Premature Senescence Upregulates the Expression of Endogenous Caveolin-1
Mol. Biol. Cell, July 1, 2002; 13(7): 2502 - 2517.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. S. Park, H. Lee, C. Riedel, J. Hulit, P. E. Scherer, R. G. Pestell, and M. P. Lisanti
Prolactin Negatively Regulates Caveolin-1 Gene Expression in the Mammary Gland during Lactation, via a Ras-dependent Mechanism
J. Biol. Chem., December 14, 2001; 276(51): 48389 - 48397.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. Wiechen, L. Diatchenko, A. Agoulnik, K. M. Scharff, H. Schober, K. Arlt, B. Zhumabayeva, P. D. Siebert, M. Dietel, R. Schafer, et al.
Caveolin-1 Is Down-Regulated in Human Ovarian Carcinoma and Acts as a Candidate Tumor Suppressor Gene
Am. J. Pathol., November 1, 2001; 159(5): 1635 - 1643.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online