| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
B in malignant
melanoma cells
1 Departments of Internal Medicine and the Cannon Research Center, Carolinas Medical Center, Charlotte 28232; 2 Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham 27710; 4 National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency, Research Triangle Park, North Carolina 27711; and 3 Division of Respiratory, Critical Care, and Occupational (Pulmonary) Medicine, University of Utah, Salt Lake City, Utah 84132
The transcription factor nuclear factor-
B (NF-B) is
constitutively activated in malignancies from enhanced activity of inhibitor of NF-B (IB) kinase, with accelerated IB
degradation. We studied whether redox signaling might stimulate these
events. Cultured melanoma cells generated superoxide anions
(O
) without serum stimulation. O
generation was reduced by the NAD(P)H:quinone oxidoreductase (NQO)
inhibitor dicumarol and the quinone analog capsaicin, suggesting that
electron transfer from NQO through a quinone-mediated pathway may be an important source of endogenous reactive oxygen species (ROS) in tumor
cells. Treatment of malignant melanoma cells with the
H2O2 scavenger catalase, the sulfhydryl donor
N-acetylcysteine, the glutathione peroxidase mimetic
ebselen, or dicumarol decreased NF-B activation. Catalase,
N-acetylcysteine, ebselen, dicumarol, and capsaicin also
inhibited growth of melanoma and other malignant cell lines. These
results raise the possibility that ROS produced endogenously by
mechanisms involving NQO can constitutively activate NF-B in an
autocrine fashion and suggest the potential for new antioxidant
strategies for interruption of oxidant signaling of melanoma cell growth.
nuclear factor-B; superoxide anion; hydrogen peroxide; tumor; neoplasm; dicumarol
This article has been cited by other articles:
|
|
 |
|
  A. Hernandez, G. Lopez-Lluch, J. A. Bernal, P. Navas, and J. A. Pintor-Toro Dicoumarol down-regulates human PTTG1/Securin mRNA expression through inhibition of Hsp90 Mol. Cancer Ther., March 1, 2008; 7(3): 474 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. M. KIM, J.-T. HWANG, D. W. KWAK, Y. K. LEE, and O. J. PARK Involvement of AMPK Signaling Cascade in Capsaicin-Induced Apoptosis of HT-29 Colon Cancer Cells Ann. N.Y. Acad. Sci., January 1, 2007; 1095(1): 496 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Qanungo, M. Wang, and A.-L. Nieminen N-Acetyl-L-cysteine Enhances Apoptosis through Inhibition of Nuclear Factor-{kappa}B in Hypoxic Murine Embryonic Fibroblasts J. Biol. Chem., November 26, 2004; 279(48): 50455 - 50464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Rodrigo, W. Passalacqua, J. Araya, M. Orellana, and G. Rivera Homocysteine and Essential Hypertension J. Clin. Pharmacol., December 1, 2003; 43(12): 1299 - 1306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Brar, Z. Corbin, T. P. Kennedy, R. Hemendinger, L. Thornton, B. Bommarius, R. S. Arnold, A. R. Whorton, A. B. Sturrock, T. P. Huecksteadt, et al. NOX5 NAD(P)H oxidase regulates growth and apoptosis in DU 145 prostate cancer cells Am J Physiol Cell Physiol, August 1, 2003; 285(2): C353 - C369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. S. Sood, M. J. Hunt, and S. C. Tyagi Peroxisome proliferator ameliorates endothelial dysfunction in a murine model of hyperhomocysteinemia Am J Physiol Lung Cell Mol Physiol, February 1, 2003; 284(2): L333 - L341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. X. Zhang, A.-P. Zou, and P.-L. Li Ceramide-induced activation of NADPH oxidase and endothelial dysfunction in small coronary arteries Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H605 - H612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Hunt and S. C. Tyagi Peroxisome proliferators compete and ameliorate Hcy-mediated endocardial endothelial cell activation Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1073 - C1079. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Surh More Than Spice: Capsaicin in Hot Chili Peppers Makes Tumor Cells Commit Suicide J Natl Cancer Inst, September 4, 2002; 94(17): 1263 - 1265. [Full Text] [PDF]
|
|
|
|
|
|
V. S. Mujumdar, C. M. Tummalapalli, G. M. Aru, and S. C. Tyagi Mechanism of constrictive vascular remodeling by homocysteine: role of PPAR Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1009 - C1015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Brar, T. P. Kennedy, A. B. Sturrock, T. P. Huecksteadt, M. T. Quinn, A. R. Whorton, and J. R. Hoidal An NAD(P)H oxidase regulates growth and transcription in melanoma cells Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1212 - C1224. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
