Am J Physiol Cell Physiol Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 280: C556-C564, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Putnam, A. J.
Right arrow Articles by Mooney, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Putnam, A. J.
Right arrow Articles by Mooney, D. J.
Vol. 280, Issue 3, C556-C564, March 2001

Control of microtubule assembly by extracellular matrix and externally applied strain

A. J. Putnam1, K. Schultz1, and D. J. Mooney1,2,3

Departments of 1 Chemical Engineering, 2 Biomedical Engineering, and 3 Biologic and Materials Sciences, University of Michigan, Ann Arbor, Michigan 48109-2136

A number of studies have suggested that externally applied mechanical forces and alterations in the intrinsic cell-extracellular matrix (ECM) force balance equivalently induce changes in cell phenotype. However, this possibility has never been directly tested. To test this hypothesis, we directly investigated the response of the microtubule (MT) cytoskeleton in smooth muscle cells to both mechanical signals and alterations in the ECM. A tensile force that resulted in a positive 10% step change in substrate strain increased MT mass by 34 ± 10% over static controls, independent of the cell adhesion ligand and tyrosine phosphorylation. Conversely, a compressive force that resulted in a negative 10% step change in substrate strain decreased MT mass by 40 ± 6% over static controls. In parallel, increasing the density of the ECM ligand fibronectin from 50 to 1,000 ng/cm2 in the absence of any applied force increased the amount of polymeric tubulin in the cell from 59 ± 11% to 81 ± 13% of the total cellular tubulin. These data are consistent with a model in which MT assembly is, in part, controlled by forces imposed on these structures, and they suggest a novel control point for MT assembly by altering the intrinsic cell-ECM force balance and applying external mechanical forces.

cytoskeleton; mechanotransduction; smooth muscle cells; tubulin; tensegrity


This article has been cited by other articles:


Home page
 Am. J. Respir. Cell Mol. Bio.Home page
R. C. Geiger, C. D. Kaufman, A. P. Lam, G. R. S. Budinger, and D. A. Dean
Tubulin Acetylation and Histone Deacetylase 6 Activity in the Lung under Cyclic Load
Am. J. Respir. Cell Mol. Biol., January 1, 2009; 40(1): 76 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. B. Khatiwala, S. R. Peyton, and A. J. Putnam
Intrinsic mechanical properties of the extracellular matrix affect the behavior of pre-osteoblastic MC3T3-E1 cells
Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1640 - C1650.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
N. D. Searby, C. R. Steele, and R. K. Globus
Influence of increased mechanical loading by hypergravity on the microtubule cytoskeleton and prostaglandin E2 release in primary osteoblasts
Am J Physiol Cell Physiol, July 1, 2005; 289(1): C148 - C158.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. KJAeR
Role of Extracellular Matrix in Adaptation of Tendon and Skeletal Muscle to Mechanical Loading
Physiol Rev, April 1, 2004; 84(2): 649 - 698.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. R. Polte, G. S. Eichler, N. Wang, and D. E. Ingber
Extracellular matrix controls myosin light chain phosphorylation and cell contractility through modulation of cell shape and cytoskeletal prestress
Am J Physiol Cell Physiol, March 1, 2004; 286(3): C518 - C528.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. E. Ingber
Tensegrity II. How structural networks influence cellular information processing networks
J. Cell Sci., April 15, 2003; 116(8): 1397 - 1408.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. E. Ingber
Tensegrity I. Cell structure and hierarchical systems biology
J. Cell Sci., April 1, 2003; 116(7): 1157 - 1173.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. J. Putnam, J. J. Cunningham, B. B. L. Pillemer, and D. J. Mooney
External mechanical strain regulates membrane targeting of Rho GTPases by controlling microtubule assembly
Am J Physiol Cell Physiol, March 1, 2003; 284(3): C627 - C639.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
L. Y. Koo, D. J. Irvine, A. M. Mayes, D. A. Lauffenburger, and L. G. Griffith
Co-regulation of cell adhesion by nanoscale RGD organization and mechanical stimulus
J. Cell Sci., January 4, 2002; 115(7): 1423 - 1433.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. P. Torchilin, R. Rammohan, V. Weissig, and T. S. Levchenko
TAT peptide on the surface of liposomes affords their efficient intracellular delivery even at low temperature and in the presence of metabolic inhibitors
PNAS, July 17, 2001; 98(15): 8786 - 8791.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online