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2- and
1-subunit abundance and mRNA in rat skeletal
muscle
1 Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles, California 90089; and 2 Institute of Physiology, University of Aarhus, 8000 Aarhus C, Denmark
Fourteen-day adrenal steroid treatment increases
[3H]ouabain binding sites 22-48% in muscle biopsies
from patients treated with adrenal steroids for chronic obstructive
lung disease and in rats treated with dexamethasone (Dex). Ouabain
binding measures plasma membrane sodium pumps
(Na+-K+-ATPase) with isoform-dependent
affinity. In this study we have established the specific pattern of Dex
regulation of sodium pump isoform protein and mRNA levels in muscle.
Rats were infused with Dex (0.1 mg/kg per day) or vehicle for 14 days.
Abundance of sodium pump catalytic 
1- and
2-subunits and glycoprotein 
1- and
2-subunits was determined by immunoblot in soleus,
extensor digitorum longus, whole gastrocnemius, and diaphragm and was
normalized to the mean vehicle control value. Dex increased
2 and 1 protein in all muscle types by
53-78% and ~50%, respectively. Dex increased 1 protein only in diaphragm (65 ± 7%). At the mRNA level in whole hindlimb muscle, Dex increased 2 (6.4 ± 0.5-fold)
and 1 (1.54 ± 0.15-fold) and decreased
2 (to 0.36 ± 0.6 of control). In summary, 21 is the Dex-responsive pump in all
skeletal muscles, and changes in 2 and 1
mRNA levels can drive the 50% change in
21-subunits, which can account for the
reported increase in [3H]ouabain binding.
Na+-K+-ATPase isoforms; dexamethasone; soleus; extensor digitorum longus; gastrocnemius; diaphragm
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