Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 280: C394-C407, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MacPherson, M. R.
Right arrow Articles by Davies, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MacPherson, M. R.
Right arrow Articles by Davies, S. A.
Vol. 280, Issue 2, C394-C407, February 2001

SPECIAL TOPIC
Model Organisms: New Insights Into Ion Channel and Transporter Function.
L-type calcium channels regulate epithelial fluid transport in Drosophila melanogaster

Matthew R. MacPherson, Valerie P. Pollock, Kate E. Broderick, Laura Kean, Fiona C. O'Connell, Julian A. T. Dow, and Shireen A. Davies

Institute of Biomedical and Life Sciences, Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, United Kingdom

The neuropeptide CAP2b stimulates fluid transport obligatorily via calcium entry, nitric oxide, and cGMP in Drosophila melanogaster Malpighian (renal) tubules. We have shown by RT-PCR that the Drosophila L-type calcium channel alpha 1-subunit genes Dmca1D and Dmca1A (nbA) are both expressed in tubules. CAP2b-stimulated fluid transport and cytosolic calcium concentration ([Ca2+]i) increases are inhibited by the L-type calcium channel blockers verapamil and nifedipine. cGMP-stimulated fluid transport is verapamil and nifedipine sensitive. Furthermore, cGMP induces a slow [Ca2+]i increase in tubule principal cells via verapamil- and nifedipine-sensitive calcium entry; RT-PCR shows that tubules express Drosophila cyclic nucleotide-gated channel (cng). Additionally, thapsigargin-induced [Ca2+]i increase is verapamil sensitive. Phenylalkylamines bind with differing affinities to the basolateral and apical surfaces of principal cells in the main segment; however, dihydropyridine binds apically in the tubule initial segment. Immunocytochemical evidence suggests localization of alpha 1-subunits to both basolateral and apical surfaces of principal cells in the tubule main segment. We suggest roles for L-type calcium channels and cGMP-mediated calcium influx in both calcium signaling and fluid transport mechanisms in Drosophila.

calcium channel alpha 1-subunits; targeted aequorin expression; intracellular calcium; guanosine 3',5'-cyclic monophosphate; cyclic nucleotide-gated channels


This article has been cited by other articles:


Home page
 J. Exp. Biol.Home page
S. A. Davies and S. Terhzaz
Organellar calcium signalling mechanisms in Drosophila epithelial function
J. Exp. Biol., February 1, 2009; 212(3): 387 - 400.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. R. MacPherson, V. P. Pollock, L. Kean, T. D. Southall, M. E. Giannakou, K. E. Broderick, J. A. T. Dow, R. C. Hardie, and S. A. Davies
Transient Receptor Potential-Like Channels Are Essential for Calcium Signaling and Fluid Transport in a Drosophila Epithelium
Genetics, March 1, 2005; 169(3): 1541 - 1552.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
V. P. Pollock, J. McGettigan, P. Cabrero, I. M. Maudlin, J. A. T. Dow, and S.-A. Davies
Conservation of capa peptide-induced nitric oxide signalling in Diptera
J. Exp. Biol., November 1, 2004; 207(23): 4135 - 4145.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
K. E. Broderick, M. R. MacPherson, M. Regulski, T. Tully, J. A. T. Dow, and S. A. Davies
Interactions between epithelial nitric oxide signaling and phosphodiesterase activity in Drosophila
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1207 - C1218.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. A. T. DOW and S. A. DAVIES
Integrative Physiology and Functional Genomics of Epithelial Function in a Genetic Model Organism
Physiol Rev, July 1, 2003; 83(3): 687 - 729.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
V. P. Pollock, J. C. Radford, S. Pyne, G. Hasan, J. A. T. Dow, and S.-A. Davies
norpA and itpr mutants reveal roles for phospholipase C and inositol (1,4,5)- trisphosphate receptor in Drosophila melanogaster renal function
J. Exp. Biol., March 1, 2003; 206(5): 901 - 911.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
P. Cabrero, J. C. Radford, K. E. Broderick, L. Costes, J. A. Veenstra, E. P. Spana, S. A. Davies, and J. A. T. Dow
The Dh gene of Drosophila melanogaster encodes a diuretic peptide that acts through cyclic AMP
J. Exp. Biol., December 15, 2002; 205(24): 3799 - 3807.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. C. Radford, S. A. Davies, and J. A. T. Dow
Systematic G-protein-coupled Receptor Analysis in Drosophila melanogaster Identifies a Leucokinin Receptor with Novel Roles
J. Biol. Chem., October 4, 2002; 277(41): 38810 - 38817.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online