Am J Physiol Cell Physiol Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 280: C309-C316, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (23)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eddinger, T. J.
Right arrow Articles by Meer, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eddinger, T. J.
Right arrow Articles by Meer, D. P.
Vol. 280, Issue 2, C309-C316, February 2001

Single rabbit stomach smooth muscle cell myosin heavy chain SMB expression and shortening velocity

Thomas J. Eddinger1 and Daniel P. Meer2

1 Department of Biology, Marquette University, Milwaukee 5320l-1881 and 2 Department of Biology, Cardinal Stritch University, Milwaukee, Wisconsin 53217

Isolated single smooth muscle cells (SMCs) from different regions of the rabbit stomach were used to determine a possible correlation between unloaded shortening velocity and smooth muscle (SM) myosin heavy chain (MHC) S1 head isoform composition (SMA, no head insert; SMB, with head insert). alpha -Toxin-permeabilized isolated single cells were maximally activated to measure unloaded shortening velocity and subsequently used in an RT-PCR reaction to determine the SMA/SMB content of the same cell. SM MHC SMA and SMB isoforms are uniquely distributed in the stomach with cells from the fundic region expressing little SMB (38.1 ± 7.3% SMB; n = 16); cells from the antrum express primarily SMB (94.9 ± 1.0% SMB; n = 16). Mean fundic cell unloaded shortening velocity was 0.014 ± 0.002 cell lengths/s compared with 0.036 ± 0.002 for the antrum cells. Unloaded shortening velocity in these cells was significantly correlated with their percent SMB expression (r2 = 0.58). Resting cell length does not correlate with the percent SMB expression (n = 32 cells). Previously published assays of purified or expressed SMA and SMB heavy meromyosin show a twofold difference in actin filament sliding speed in in vitro motility assays. Extrapolation of our data to 0-100% SMB would give a 10-fold range of shortening velocity, which is closer to the ~20-fold range reported from various SM tissues. This suggests that mechanisms in addition to the MHC S1 head isoforms regulate shortening velocity.

muscle mechanics; reverse transcriptase-polymerase chain reaction


This article has been cited by other articles:


Home page
 Proc Am Thorac SocHome page
R. Leguillette and A.-M. Lauzon
Molecular Mechanics of Smooth Muscle Contractile Proteins in Airway Hyperresponsiveness and Asthma
Proceedings of the ATS, January 1, 2008; 5(1): 40 - 46.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. J. Eddinger and D. P. Meer
Myosin II isoforms in smooth muscle: heterogeneity and function
Am J Physiol Cell Physiol, August 1, 2007; 293(2): C493 - C508.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. J. Eddinger, J. D. Schiebout, and D. R. Swartz
Adherens junction-associated protein distribution differs in smooth muscle tissue and acutely isolated cells
Am J Physiol Gastrointest Liver Physiol, February 1, 2007; 292(2): G684 - G697.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
F. R. Gil, N. B. Zitouni, E. Azoulay, K. Maghni, and A.-M. Lauzon
Smooth muscle myosin isoform expression and LC20 phosphorylation in innate rat airway hyperresponsiveness
Am J Physiol Lung Cell Mol Physiol, November 1, 2006; 291(5): L932 - L940.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Basha, S. Chang, E. M. Smolock, R. S. Moreland, A. J. Wein, and S. Chacko
Regional differences in myosin heavy chain isoform expression and maximal shortening velocity of the rat vaginal wall smooth muscle
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2006; 291(4): R1076 - R1084.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Leguillette, F. R. Gil, N. Zitouni, S. Lajoie-Kadoch, A. Sobieszek, and A.-M. Lauzon
(+)Insert smooth muscle myosin heavy chain (SM-B) isoform expression in human tissues
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1277 - C1285.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Wahl, T. J. Eddinger, and C.-M. Hai
Sinusoidal length oscillation- and receptor-mediated mRNA expression of myosin isoforms and {alpha}-SM actin in airway smooth muscle
Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1697 - C1708.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. J. Babu, G. J. Pyne, Y. Zhou, C. Okwuchukuasanya, J. E. Brayden, G. Osol, R. J. Paul, R. B. Low, and M. Periasamy
Isoform switching from SM-B to SM-A myosin results in decreased contractility and altered expression of thin filament regulatory proteins
Am J Physiol Cell Physiol, September 1, 2004; 287(3): C723 - C729.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
K.-E. Andersson and A. Arner
Urinary Bladder Contraction and Relaxation: Physiology and Pathophysiology
Physiol Rev, July 1, 2004; 84(3): 935 - 986.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. A. Tuck, K. Maghni, A. Poirier, G. J. Babu, M. Periasamy, J. H. T. Bates, R. Leguillette, and A.-M. Lauzon
Time Course of Airway Mechanics of the (+)Insert Myosin Isoform Knockout Mouse
Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 326 - 332.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. E. DiSanto, R. Stein, S. Chang, J. A. Hypolite, Y. Zheng, S. Zderic, A. J. Wein, and S. Chacko
Alteration in expression of myosin isoforms in detrusor smooth muscle following bladder outlet obstruction
Am J Physiol Cell Physiol, December 1, 2003; 285(6): C1397 - C1410.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. Karagiannis, G. J Babu, M. Periasamy, and F. V Brozovich
The Smooth Muscle Myosin Seven Amino Acid Heavy Chain Insert's Kinetic Role in the Crossbridge Cycle for Mouse Bladder
J. Physiol., March 1, 2003; 547(2): 463 - 473.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J. A. Parisi and T. J. Eddinger
Smooth Muscle Myosin Heavy Chain Isoform Distribution in the Swine Stomach
J. Histochem. Cytochem., March 1, 2002; 50(3): 385 - 394.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. Khatri, K. M. Joyce, F. V. Brozovich, and S. A. Fisher
Role of Myosin Phosphatase Isoforms in cGMP-mediated Smooth Muscle Relaxation
J. Biol. Chem., September 28, 2001; 276(40): 37250 - 37257.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
A. S. Rovner, P. M. Fagnant, S. Lowey, and K. M. Trybus
The carboxyl-terminal isoforms of smooth muscle myosin heavy chain determine thick filament assembly properties
J. Cell Biol., January 7, 2002; 156(1): 113 - 124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. J. Sherwood and T. J. Eddinger
Shortening velocity and myosin heavy- and light-chain isoform mRNA in rabbit arterial smooth muscle cells
Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1093 - C1102.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online