Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 280: C288-C295, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (55)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dennis, R. G.
Right arrow Articles by Faulkner, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dennis, R. G.
Right arrow Articles by Faulkner, J. A.
Vol. 280, Issue 2, C288-C295, February 2001

Excitability and contractility of skeletal muscle engineered from primary cultures and cell lines

Robert G. Dennis, Paul E. Kosnik II, Mark E. Gilbert, and John A. Faulkner

1 Muscle Mechanics Laboratory, Institute of Gerontology, University of Michigan, Ann Arbor 48109-2007; and 2 Gilbert Engineering, Inc., Ann Arbor, Michigan 48103-9005

The purpose of this study was to compare the excitability and contractility of three-dimensional skeletal muscle constructs, termed myooids, engineered from C2C12 myoblast and 10T1/2 fibroblast cell lines, primary muscle cultures from adult C3H mice, and neonatal and adult Sprague-Dawley rats. Myooids were 12 mm long, with diameters of 0.1-1 mm, were excitable by transverse electrical stimulation, and contracted to produce force. After ~30 days in culture, myooid cross-sectional area, rheobase, chronaxie, resting baseline force, twitch force, time to peak tension, one-half relaxation time, and peak isometric force were measured. Specific force was calculated by dividing peak isometric force by cross-sectional area. The specific force generated by the myooids was 2-8% of that generated by skeletal muscles of control adult rodents. Myooids engineered from C2C12-10T1/2 cells exhibited greater rheobase, time to peak tension, and one-half relaxation time than myooids engineered from adult rodent cultures, and myooids from C2C12-10T1/2 and neonatal rat cells had greater resting baseline forces than myooids from adult rodent cultures.

tissue engineering; myooid; myogenesis; isometric force; rodent tissue culture


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
K. J. M. Boonen, K. Y. Rosaria-Chak, F. P. T. Baaijens, D. W. J. van der Schaft, and M. J. Post
Essential environmental cues from the satellite cell niche: optimizing proliferation and differentiation
Am J Physiol Cell Physiol, June 1, 2009; 296(6): C1338 - C1345.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Gawlitta, C. W. J. Oomens, D. L. Bader, F. P. T. Baaijens, and C. V. C. Bouten
Temporal differences in the influence of ischemic factors and deformation on the metabolism of engineered skeletal muscle
J Appl Physiol, August 1, 2007; 103(2): 464 - 473.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Hecker, K. Baar, R. G. Dennis, and K. N. Bitar
Development of a three-dimensional physiological model of the internal anal sphincter bioengineered in vitro from isolated smooth muscle cells
Am J Physiol Gastrointest Liver Physiol, August 1, 2005; 289(2): G188 - G196.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y.-C. Huang, R. G. Dennis, L. Larkin, and K. Baar
Rapid formation of functional muscle in vitro using fibrin gels
J Appl Physiol, February 1, 2005; 98(2): 706 - 713.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online