Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 280: C280-C287, 2001;
0363-6143/01 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (18)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Otto, C. M.
Right arrow Articles by Baumgardner, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Otto, C. M.
Right arrow Articles by Baumgardner, J. E.
Vol. 280, Issue 2, C280-C287, February 2001

Effect of culture PO2 on macrophage (RAW 264.7) nitric oxide production

Cynthia M. Otto1 and James E. Baumgardner2,3

Departments of 1 Clinical Studies-Philadelphia and 2 Anesthesia, University of Pennsylvania, Philadelphia 19104; and 3 SpectruMedix, State College, Pennsylvania 16803

Macrophages are commonly cultured at a PO2 of 149 Torr, but tissue macrophages in vivo live in an environment of much lower oxygen tension. Despite the many potential mechanisms for changes in oxygen tension to influence nitric oxide (NO) synthesis, there have been few reports investigating the effect of PO2 on macrophage NO production. With the use of a culture chamber designed to rigorously control oxygen tension, we investigated the effects of culture PO2 on macrophage NO production, inducible nitric oxide synthase (iNOS) activity, iNOS protein, and tumor necrosis factor production. NO production and iNOS activity were linearly related in the range of 39.4 to 677 Torr, but not in the range of 1.03 to 39.4 Torr. Therefore, results obtained in vitro for the high oxygen tensions commonly used in cell culture were quantitatively and qualitatively different from results obtained in cells cultured at the lower oxygen tensions that more accurately reflect the in vivo environment. The influence of oxygen tension on NO production has implications for cell culture methodology and for the relationship between microcirculatory dysfunction and inflammatory responses in rodent models of sepsis.

inducible nitric oxide synthase; hypoxia; diffusion; inflammation; tumor necrosis factor


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
M. A. Robinson, J. E. Baumgardner, V. P. Good, and C. M. Otto
Physiological and hypoxic O2 tensions rapidly regulate NO production by stimulated macrophages
Am J Physiol Cell Physiol, April 1, 2008; 294(4): C1079 - C1087.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
R. J. Anand, S. C. Gribar, J. Li, J. W. Kohler, M. F. Branca, T. Dubowski, C. P. Sodhi, and D. J. Hackam
Hypoxia causes an increase in phagocytosis by macrophages in a HIF-1{alpha}-dependent manner
J. Leukoc. Biol., November 1, 2007; 82(5): 1257 - 1265.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
A. Degrossoli and S. Giorgio
Functional Alterations in Macrophages After Hypoxia Selection
Experimental Biology and Medicine, January 1, 2007; 232(1): 88 - 95.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. C. Land and C. Rae
iNOS initiates and sustains metabolic arrest in hypoxic lung adenocarcinoma cells: mechanism of cell survival in solid tumor core
Am J Physiol Cell Physiol, October 1, 2005; 289(4): C918 - C933.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. Takizawa, H. Yoshikawa, M. Yamada, and H. Morita
Expression of nitric oxide synthase isoforms and detection of nitric oxide in rat placenta
Am J Physiol Cell Physiol, April 1, 2002; 282(4): C762 - C767.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online