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Am J Physiol Cell Physiol 280: C175-C182, 2001;
0363-6143/01 $5.00
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Vol. 280, Issue 1, C175-C182, January 2001

Properties of voltage-gated Ca2+ channels in rabbit ventricular myocytes expressing Ca2+ channel alpha 1E cDNA

Michihiro Tateyama1, Shuqin Zong2, Tsutomu Tanabe2, and Rikuo Ochi1

1 Department of Physiology, Juntendo University School of Medicine, Tokyo 113-8421; and 2 Department of Pharmacology and Neurobiology, Graduate School of Medicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8519, Japan

Using the whole-cell patch-clamp technique, we have studied the properties of alpha 1E Ca2+ channel transfected in cardiac myocytes. We have also investigated the effect of foreign gene expression on the intrinsic L-type current (ICa,L). Expression of green fluorescent protein significantly decreased the ICa,L. By contrast, expression of alpha 1E with beta 2b and alpha 2/delta significantly increased the total Ca2+ current, and in these cells a Ca2+ antagonist, PN-200-110 (PN), only partially blocked the current. The remaining PN-resistant current was abolished by the application of a low concentration of Ni2+ and was little affected by changing the charge carrier from Ca2+ to Ba2+ or by beta -adrenergic stimulation. On the basis of its voltage range for activation, this channel was classified as a high-voltage activated channel. Thus the expression of alpha 1E did not generate T-like current in cardiac myocytes. On the other hand, expression of alpha 1E decreased ICa,L and slowed the ICa,L inactivation. This inactivation slowing was attenuated by the beta 2b coexpression, suggesting that the alpha 1E may slow the inactivation of ICa,L by scrambling with alpha 1C for intrinsic auxiliary beta .

green fluorescent protein; culture; L-type calcium channel; calcium channel beta -subunit; transfection


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