Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 279: C1744-C1750, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Song, Y.
Right arrow Articles by Verkman, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Song, Y.
Right arrow Articles by Verkman, A. S.
Vol. 279, Issue 6, C1744-C1750, December 2000

Role of aquaporin water channels in pleural fluid dynamics

Yuanlin Song, Baoxue Yang, Michael A. Matthay, Tonghui Ma, and A. S. Verkman

Departments of Medicine and Physiology, Cardiovascular Research Institute, University of California, San Francisco, California 94143-0521

Continuous movement of fluid into and out of the pleural compartment occurs in normal chest physiology and in pathophysiological conditions associated with pleural effusions. RT-PCR screening and immunostaining revealed expression of water channel aquaporin-1 (AQP1) in microvascular endothelia near the visceral and parietal pleura and in mesothelial cells in visceral pleura. Comparative physiological measurements were done on wild-type vs. AQP1 null mice. Osmotically driven water transport was measured in anesthetized, mechanically ventilated mice from the kinetics of pleural fluid osmolality after instillation of 0.25 ml of hypertonic or hypotonic fluid into the pleural space. Osmotic equilibration of pleural fluid was rapid in wild-type mice (50% equilibration in <2 min) and remarkably slowed by greater than fourfold in AQP1 null mice. Small amounts of AQP3 transcript were also detected in pleura by RT-PCR, but osmotic water transport was not decreased in AQP3 null mice. In spontaneously breathing mice, the clearance of isosmolar saline instilled in the pleural space (~4 ml · kg-1 · h-1) was not affected by AQP1 deletion. In a fluid overload model produced by intraperitoneal saline administration and renal artery ligation, the accumulation of pleural fluid (~0.035 ml/h) and was not affected by AQP1 deletion. Finally, in a thiourea toxicity model of acute endothelial injury causing pleural effusions and lung interstitial edema, pleural fluid accumulation in the first 3 h (~4 ml · kg-1 · h-1) was not affected by AQP1 deletion. These results indicate rapid osmotic equilibration across the pleural surface that is facilitated by AQP1 water channels. However, AQP1 does not appear to play a role in clinically relevant mechanisms of pleural fluid accumulation or clearance.

lung; pleura; water transport; aquaporin-1; pleural effusion


This article has been cited by other articles:


Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. Su, M. R. Looney, N. Gupta, and M. A. Matthay
Receptor for advanced glycation end-products (RAGE) is an indicator of direct lung injury in models of experimental lung injury
Am J Physiol Lung Cell Mol Physiol, July 1, 2009; 297(1): L1 - L5.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
L. Li, H. Zhang, T. Ma, and A. S. Verkman
Very high aquaporin-1 facilitated water permeability in mouse gallbladder
Am J Physiol Gastrointest Liver Physiol, April 1, 2009; 296(4): G816 - G822.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
M. S. Stoenoiu, J. Ni, C. Verkaeren, H. Debaix, J.-C. Jonas, N. Lameire, J.-M. Verbavatz, and O. Devuyst
Corticosteroids Induce Expression of Aquaporin-1 and Increase Transcellular Water Transport in Rat Peritoneum
J. Am. Soc. Nephrol., March 1, 2003; 14(3): 555 - 565.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
L. Zocchi
Physiology and pathophysiology of pleural fluid turnover
Eur. Respir. J., December 1, 2002; 20(6): 1545 - 1558.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Z. Borok and A. S. Verkman
Lung Edema Clearance: 20 Years of Progress: Invited Review: Role of aquaporin water channels in fluid transport in lung and airways
J Appl Physiol, December 1, 2002; 93(6): 2199 - 2206.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online