Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 279: C1558-C1563, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (34)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stevens, L.
Right arrow Articles by Pette, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stevens, L.
Right arrow Articles by Pette, D.
Vol. 279, Issue 5, C1558-C1563, November 2000

Effects of unweighting and clenbuterol on myosin light and heavy chains in fast and slow muscles of rat

Laurence Stevens1, Carole Firinga1, Bärbel Gohlsch2, Bruno Bastide1, Yvonne Mounier1, and Dirk Pette2

1 Laboratoire de Plasticité Neuromusculaire, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq, France; and 2 Faculty of Biology, University of Constance, D-78457 Constance, Germany

To investigate the plasticity of slow and fast muscles undergoing slow-to-fast transition, rat soleus (SOL), gastrocnemius (GAS), and extensor digitorum longus (EDL) muscles were exposed for 14 days to 1) unweighting by hindlimb suspension (HU), or 2) treatment with the beta 2-adrenergic agonist clenbuterol (CB), or 3) a combination of both (HU-CB). In general, HU elicited atrophy, CB induced hypertrophy, and HU-CB partially counteracted the HU-induced atrophy. Analyses of myosin heavy (MHC) and light chain (MLC) isoforms revealed HU- and CB-induced slow-to-fast transitions in SOL (increases of MHCIIa with small amounts of MHCIId and MHCIIb) and the upregulation of the slow MHCIa isoform. The HU- and CB-induced changes in GAS consisted of increases in MHCIId and MHCIIb ("fast-to-faster transitions"). Changes in the MLC composition of SOL and GAS consisted of slow-to-fast transitions and mainly encompassed an exchange of MLC1s with MLC1f. In addition, MLC3f was elevated whenever MHCIId and MHCIIb isoforms were increased. Because the EDL is predominantly composed of type IID and IIB fibers, HU, CB, and HU-CB had no significant effect on the MHC and MLC patterns.

extensor digitorum longus; gastrocnemius; hindlimb suspension; myosin; slow-to-fast transition; soleus


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
H. S. Rhee, C. M. Steel, F. J. Derksen, N. E. Robinson, and J. F.Y. Hoh
Immunohistochemical Analysis of Laryngeal Muscles in Normal Horses and Horses With Subclinical Recurrent Laryngeal Neuropathy
J. Histochem. Cytochem., August 1, 2009; 57(8): 787 - 800.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. C. I. Wust, R. T. Jaspers, A. F. van Heijst, M. T. E. Hopman, L. J. C. Hoofd, W. J. van der Laarse, and H. Degens
Region-specific adaptations in determinants of rat skeletal muscle oxygenation to chronic hypoxia
Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H364 - H374.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. S. Lynch and J. G. Ryall
Role of {beta}-Adrenoceptor Signaling in Skeletal Muscle: Implications for Muscle Wasting and Disease
Physiol Rev, April 1, 2008; 88(2): 729 - 767.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
G. Fuster, S. Busquets, E. Ametller, M. Olivan, V. Almendro, C. C. Fontes de Oliveira, M. Figueras, F. J. Lopez-Soriano, and J. M. Argiles
Are Peroxisome Proliferator-Activated Receptors Involved in Skeletal Muscle Wasting during Experimental Cancer Cachexia? Role of {beta}2-Adrenergic Agonists
Cancer Res., July 1, 2007; 67(13): 6512 - 6519.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Yimlamai, S. L. Dodd, S. E. Borst, and S. Park
Clenbuterol induces muscle-specific attenuation of atrophy through effects on the ubiquitin-proteasome pathway
J Appl Physiol, July 1, 2005; 99(1): 71 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Bozzo, L. Stevens, L. Toniolo, Y. Mounier, and C. Reggiani
Increased phosphorylation of myosin light chain associated with slow-to-fast transition in rat soleus
Am J Physiol Cell Physiol, September 1, 2003; 285(3): C575 - C583.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
G. Gayan-Ramirez and M. Decramer
Effects of mechanical ventilation on diaphragm function and biology
Eur. Respir. J., December 1, 2002; 20(6): 1579 - 1586.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F. Picquet, V. Bouet, M. H. Canu, L. Stevens, Y. Mounier, M. Lacour, and M. Falempin
Contractile properties and myosin expression in rats born and reared in hypergravity
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1687 - R1695.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. A. Timmons
Can we "switch" the emphasis please?
J Appl Physiol, May 1, 2002; 92(5): 2221 - 2221.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. N. Smith, A. Dirks, T. Sugiura, S. Muller, P. Scarpace, and S. K. Powers
Alteration of contractile force and mass in the senescent diaphragm with beta 2-agonist treatment
J Appl Physiol, March 1, 2002; 92(3): 941 - 948.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Stevens, B. Bastide, P. Kischel, D. Pette, and Y. Mounier
Time-dependent changes in expression of troponin subunit isoforms in unloaded rat soleus muscle
Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1025 - C1030.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online