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Am J Physiol Cell Physiol 279: C1425-C1431, 2000;
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Vol. 279, Issue 5, C1425-C1431, November 2000

Molecular characterization of a novel UT-A urea transporter isoform (UT-A5) in testis

R. A. Fenton1, A. Howorth1, G. J. Cooper1, R. Meccariello2, I. D. Morris1, and C. P. Smith1

1 School of Biological Science, University of Manchester, Manchester M13 9PT, United Kingdom; and 2 Seconda Universita' degli Studi di Napoli, Facolta' di Medicina e Chirurgia, 1680138 Napoli, Italy

Urea movement across plasma membranes is modulated by specialized transporter proteins that are products of two genes, termed UT-A and UT-B. These proteins play key roles in the urinary concentrating mechanism and fluid homeostasis. We have isolated and characterized a 1.4-kb cDNA from testes encoding a new isoform (UT-A5) belonging to the UT-A transporter family. For comparison, we also isolated a 2.0-kb cDNA from mouse kidney inner medulla encoding the mouse UT-A3 homologue. The UT-A5 cDNA has a putative open reading frame encoding a 323-amino acid protein, making UT-A5 the smallest UT-A family member in terms of molecular size. Its putative topology is of particular interest, because it calls into question earlier models of UT-A transporter structure. Expression of UT-A5 cRNA in Xenopus oocytes mediates phloretin-inhibitable urea uptake and does not translocate water. The distribution of UT-A5 mRNA is restricted to the peritubular myoid cells forming the outermost layer of the seminiferous tubules within the testes and is not detected in kidney. UT-A5 mRNA levels are coordinated with the stage of testes development and increase 15 days postpartum, commensurate with the start of seminiferous tubule fluid movement.

urea transporter; membrane protein; testes; mouse


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