Am J Physiol Cell Physiol AJP: Advances in Physiology Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 279: C935-C944, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fischer, S.
Right arrow Articles by Schaper, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fischer, S.
Right arrow Articles by Schaper, W.
Vol. 279, Issue 4, C935-C944, October 2000

Effect of astroglial cells on hypoxia-induced permeability in PBMEC cells

Silvia Fischer1, Maria Wobben2, Jan Kleinstück2, Dieter Renz1, and Wolfgang Schaper2

1 Department of Anesthesiology and Intensive Care and 2 Department of Experimental Cardiology, Max-Planck Institute for Physiological and Clinical Research, D-61231 Bad Nauheim, Germany

An in vitro model of the blood-brain barrier (BBB), consisting of porcine brain-derived microvascular endothelial cells (PBMEC), was used to evaluate the effect of astrocytes in the BBB disruption during hypoxia. Hypoxia-induced hyperpermeability was decreased significantly in a coculture model of astroglia cells, either astrocytes or C6 glioma cells, with PBMEC and, to the same extent, when glia cell-conditioned medium was used. Corresponding to effects on hypoxia-induced hyperpermeability, astrocyte- and C6 cell-conditioned medium diminished hypoxia-induced vascular endothelial growth factor (VEGF) mRNA and protein expression, which recently was shown to be responsible for hypoxia-induced permeability changes in vitro. The effect on hypoxia-induced hyperpermeability and VEGF expression was specific for astroglia cells because conditioned medium from bovine smooth muscle cells (BSMC) did not show any effect. Immunocytochemistry revealed that 24 h of hypoxia disrupted the continuity of the tight junction protein, zonula occludens-1 (ZO-1), which lines the cytoplasmic face of intact tight junctions. These changes were prevented when hypoxia was performed in glia cell-conditioned medium. Results suggest that astrocytes protect the BBB from hypoxia-induced paracellular permeability changes by decreasing hypoxia-induced VEGF expression in microvascular endothelial cells.

porcine brain microvascular endothelial cells; vascular endothelial growth factor; astrocytes


This article has been cited by other articles:


Home page
 StrokeHome page
M. A. Yenari, L. Xu, X. N. Tang, Y. Qiao, and R. G. Giffard
Microglia Potentiate Damage to Blood-Brain Barrier Constituents: Improvement by Minocycline In Vivo and In Vitro
Stroke, April 1, 2006; 37(4): 1087 - 1093.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
B. T. Hawkins and T. P. Davis
The Blood-Brain Barrier/Neurovascular Unit in Health and Disease
Pharmacol. Rev., June 1, 2005; 57(2): 173 - 185.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
X. Gu, J. Zhang, D. W. Brann, and F.-S. X. Yu
Brain and Retinal Vascular Endothelial Cells with Extended Life Span Established by Ectopic Expression of Telomerase
Invest. Ophthalmol. Vis. Sci., July 1, 2003; 44(7): 3219 - 3225.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. C. Brown, K. S. Mark, R. D. Egleton, J. D. Huber, A. R. Burroughs, and T. P. Davis
Protection against hypoxia-induced increase in blood-brain barrier permeability: role of tight junction proteins and NF{kappa}B
J. Cell Sci., February 15, 2003; 116(4): 693 - 700.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. S. Mark and T. P. Davis
Cerebral microvascular changes in permeability and tight junctions induced by hypoxia-reoxygenation
Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1485 - H1494.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online