| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of Surgery and of Ophthalmology and Visual Science, Yale University, New Haven, Connecticut 06520-8062
The retinal pigment epithelium (RPE) is an epithelial region of the blood-brain barrier. During embryogenesis, permeability of the barrier gradually decreases. A culture model of RPE development revealed differences in how tight junctions regulate the paracellular diffusion of ionic and nonionic solutes (Ban Y and Rizzolo LJ. Mol Vis 3: 18, 1997). To examine these differences, the permeation of ionic and nonionic monosaccharides was compared with mannitol, and the permeation of the alkali metals was compared with sodium. The order of permeation was 3-O-methlyglucose = glucosamine = mannitol > N-acetylneuraminic acid. The ratio of N-acetylneuraminic acid to mannitol permeability decreased with embryonic age of the RPE or exposure to retinal-conditioned medium. Neither the ratio nor the permeability was affected by inhibiting transcytosis. The ratio increased if tight junctions were disrupted in low-calcium medium. The permeation of cations followed the sequence cesium > rubidium > potassium = sodium > lithium and was unaffected by embryonic age or retinal-conditioned medium. These results are considered in terms of a model in which the size distribution, charge, or number of open junctional pores could be modulated. It suggests that different subpopulations of pores can be regulated independently during development.
blood-retinal barrier; paracellular permeability; epithelium; intercellular junction; retina
This article has been cited by other articles:
|
|
 |
|
  D. J. Grab, E. Nyarko, N. C. Barat, O. V. Nikolskaia, and J. S. Dumler Anaplasma phagocytophilum-Borrelia burgdorferi Coinfection Enhances Chemokine, Cytokine, and Matrix Metalloprotease Expression by Human Brain Microvascular Endothelial Cells Clin. Vaccine Immunol., November 1, 2007; 14(11): 1420 - 1424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Strauss The Retinal Pigment Epithelium in Visual Function Physiol Rev, July 1, 2005; 85(3): 845 - 881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wen, D. D. Watry, M. C. G. Marcondes, and H. S. Fox Selective Decrease in Paracellular Conductance of Tight Junctions: Role of the First Extracellular Domain of Claudin-5 Mol. Cell. Biol., October 1, 2004; 24(19): 8408 - 8417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Narayan, G. Prasanna, R. R. Krishnamoorthy, X. Zhang, and T. Yorio Endothelin-1 Synthesis and Secretion in Human Retinal Pigment Epithelial Cells (ARPE-19): Differential Regulation by Cholinergics and TNF-{alpha} Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 4885 - 4894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Rajasekaran, J. Hu, J. Gopal, R. Gallemore, S. Ryazantsev, D. Bok, and A. K. Rajasekaran Na,K-ATPase inhibition alters tight junction structure and permeability in human retinal pigment epithelial cells Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1497 - C1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Peng, C. Rahner, and L. J. Rizzolo Apical and Basal Regulation of the Permeability of the Retinal Pigment Epithelium Invest. Ophthalmol. Vis. Sci., February 1, 2003; 44(2): 808 - 817. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
