Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 279: C670-C681, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (75)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Himms-Hagen, J.
Right arrow Articles by Cinti, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Himms-Hagen, J.
Right arrow Articles by Cinti, S.
Vol. 279, Issue 3, C670-C681, September 2000

Multilocular fat cells in WAT of CL-316243-treated rats derive directly from white adipocytes

J. Himms-Hagen1,*, A. Melnyk1, M. C. Zingaretti2, E. Ceresi2, G. Barbatelli2, and S. Cinti2,*

1 Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa K1H 8M5, Canada; and 2 Institute of Normal Human Morphology-Anatomy, Faculty of Medicine, University of Ancona, 60020 Ancona, Italy

Multilocular, mitochondria-rich adipocytes appear in white adipose tissue (WAT) of rats treated with the beta 3-adrenoceptor agonist, CL-316243 (CL). Objectives were to determine whether these multilocular adipocytes derived from cells that already existed in the WAT or from proliferation of precursor cells and whether new mitochondria contained in them were typical brown adipocyte mitochondria. Use of 5-bromodeoxyuridine to identify cells that had undergone mitosis during the CL treatment showed that most multilocular cells derived from cells already present in the WAT. Morphological techniques showed that at least a subpopulation of unilocular adipocytes underwent conversion to multilocular mitochondria-rich adipocytes. A small proportion of multilocular adipocytes (~8%) was positive for UCP1 by immunohistochemistry. Biochemical techniques showed that mitochondrial protein recovered from WAT increased 10-fold and protein isolated from brown adipose tissue (BAT) doubled in CL-treated rats. Stained gels showed a different protein composition of new mitochondria isolated from WAT from that of mitochondria isolated from BAT. Western blotting showed new mitochondria in WAT to contain both UCP1, but at a much lower concentration than in BAT mitochondria, and UCP3, at a higher concentration than that in BAT mitochondria. We hypothesize that multilocular adipocytes present at 7 days of CL treatment have two origins. First, most come from convertible unilocular adipocytes that become multilocular and make many mitochondria that contain UCP3. Second, some come from a cell that gives rise to more typical brown adipocytes that express UCP1.

uncoupling proteins; white adipose tissue; brown adipose tissue; thermogenesis; ultrastructure; morphology; bromodeoxyuridine; mitochondrial biogenesis; obesity; beta 3-adrenoceptor; endothelial cells; brown adipocytes; convertible adipocytes

* J. Himms-Hagen and S. Cinti share equally the senior authorship of this work.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Cinti
Transdifferentiation properties of adipocytes in the adipose organ
Am J Physiol Endocrinol Metab, November 1, 2009; 297(5): E977 - E986.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. A. Everson and A. Szabo
Recurrent restriction of sleep and inadequate recuperation induce both adaptive changes and pathological outcomes
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2009; 297(5): R1430 - R1440.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Karamitri, A. M. Shore, K. Docherty, J. R. Speakman, and M. A. Lomax
Combinatorial Transcription Factor Regulation of the Cyclic AMP-response Element on the Pgc-1{alpha} Promoter in White 3T3-L1 and Brown HIB-1B Preadipocytes
J. Biol. Chem., July 31, 2009; 284(31): 20738 - 20752.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Veyrat-Durebex, X. Montet, M. Vinciguerra, A. Gjinovci, P. Meda, M. Foti, and F. Rohner-Jeanrenaud
The Lou/C rat: a model of spontaneous food restriction associated with improved insulin sensitivity and decreased lipid storage in adipose tissue
Am J Physiol Endocrinol Metab, May 1, 2009; 296(5): E1120 - E1132.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. K. Song, C. H. Vaughan, E. Keen-Rhinehart, R. B. S. Harris, D. Richard, and T. J. Bartness
Melanocortin-4 receptor mRNA expressed in sympathetic outflow neurons to brown adipose tissue: neuroanatomical and functional evidence
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R417 - R428.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. P. Mottillo, X. J. Shen, and J. G. Granneman
Role of hormone-sensitive lipase in beta-adrenergic remodeling of white adipose tissue
Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1188 - E1197.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. Wang, E. Bomberg, C. Billington, A. Levine, and C. M. Kotz
Brain-derived neurotrophic factor in the hypothalamic paraventricular nucleus increases energy expenditure by elevating metabolic rate
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R992 - R1002.
[Abstract] [Full Text] [PDF]

Home page
 Diabetes CareHome page
I. Bogacka, T. W. Gettys, L. de Jonge, T. Nguyen, J. M. Smith, H. Xie, F. Greenway, and S. R. Smith
The Effect of {beta}-Adrenergic and Peroxisome Proliferator-Activated Receptor-{gamma} Stimulation on Target Genes Related to Lipid Metabolism in Human Subcutaneous Adipose Tissue
Diabetes Care, May 1, 2007; 30(5): 1179 - 1186.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. D. Mulligan, A. A. Gonzalez, A. M. Stewart, H. V. Carey, and K. W. Saupe
Upregulation of AMPK during cold exposure occurs via distinct mechanisms in brown and white adipose tissue of the mouse
J. Physiol., April 15, 2007; 580(2): 677 - 684.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. Frontini, S. Rousset, A.-M. Cassard-Doulcier, C. Zingaretti, D. Ricquier, and S. Cinti
Thymus Uncoupling Protein 1 Is Exclusive to Typical Brown Adipocytes and Is Not Found in Thymocytes
J. Histochem. Cytochem., February 1, 2007; 55(2): 183 - 189.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
B. Xue, J.-S. Rim, J. C. Hogan, A. A. Coulter, R. A. Koza, and L. P. Kozak
Genetic variability affects the development of brown adipocytes in white fat but not in interscapular brown fat
J. Lipid Res., January 1, 2007; 48(1): 41 - 51.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. T. Foster and T. J. Bartness
Sympathetic but not sensory denervation stimulates white adipocyte proliferation
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2006; 291(6): R1630 - R1637.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. G. Granneman, P. Li, Z. Zhu, and Y. Lu
Metabolic and cellular plasticity in white adipose tissue I: effects of {beta}3-adrenergic receptor activation
Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E608 - E616.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. Li, Z. Zhu, Y. Lu, and J. G. Granneman
Metabolic and cellular plasticity in white adipose tissue II: role of peroxisome proliferator-activated receptor-{alpha}
Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E617 - E626.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Robidoux, W. Cao, H. Quan, K. W. Daniel, F. Moukdar, X. Bai, L. M. Floering, and S. Collins
Selective Activation of Mitogen-Activated Protein (MAP) Kinase Kinase 3 and p38{alpha} MAP Kinase Is Essential for Cyclic AMP-Dependent UCP1 Expression in Adipocytes
Mol. Cell. Biol., July 1, 2005; 25(13): 5466 - 5479.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Kang, L. Bajnok, K. A. Longo, R. K. Petersen, J. B. Hansen, K. Kristiansen, and O. A. MacDougald
Effects of Wnt Signaling on Brown Adipocyte Differentiation and Metabolism Mediated by PGC-1{alpha}
Mol. Cell. Biol., February 15, 2005; 25(4): 1272 - 1282.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. G. Granneman, P. Li, Y. Lu, and J. Tilak
Seeing the trees in the forest: selective electroporation of adipocytes within adipose tissue
Am J Physiol Endocrinol Metab, September 1, 2004; 287(3): E574 - E582.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. R. Bowers, W. T. L. Festuccia, C. K. Song, H. Shi, R. H. Migliorini, and T. J. Bartness
Sympathetic innervation of white adipose tissue and its regulation of fat cell number
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2004; 286(6): R1167 - R1175.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. G. Granneman, M. Burnazi, Z. Zhu, and L. A. Schwamb
White adipose tissue contributes to UCP1-independent thermogenesis
Am J Physiol Endocrinol Metab, December 1, 2003; 285(6): E1230 - E1236.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
A. A. Coulter, C. M. Bearden, X. Liu, R. A. Koza, and L. P. Kozak
Dietary fat interacts with QTLs controlling induction of Pgc-1{alpha} and Ucp1 during conversion of white to brown fat
Physiol Genomics, July 7, 2003; 14(2): 139 - 147.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online