Am J Physiol Cell Physiol Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 279: C352-C360, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamboliev, I. A.
Right arrow Articles by Gerthoffer, W. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamboliev, I. A.
Right arrow Articles by Gerthoffer, W. T.
Vol. 279, Issue 2, C352-C360, August 2000

Phosphatidylinositol 3-kinases regulate ERK and p38 MAP kinases in canine colonic smooth muscle

Ilia A. Yamboliev, Kevin M. Wiesmann, Cherie A. Singer, Jason C. Hedges, and William T. Gerthoffer

Department of Pharmacology, University of Nevada School of Medicine, Reno, Nevada 89557-0046

In canine colon, M2/M3 muscarinic receptors are coupled to extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein (MAP) kinases. We tested the hypothesis that this coupling is mediated by enzymes of the phosphatidylinositol (PI) 3-kinase family. RT-PCR and Western blotting demonstrated expression of two isoforms, PI 3-kinase-alpha and PI 3-kinase-gamma . Muscarinic stimulation of intact muscle strips (10 µM ACh) activated PI 3-kinase-gamma , ERK and p38 MAP kinases, and MAP kinase-activated protein kinase-2, whereas PI 3-kinase-alpha activation was not detected. Wortmannin (25 µM) abolished the activation of PI 3-kinase-gamma , ERK, and p38 MAP kinases. MAP kinase inhibition was a PI 3-kinase-gamma -specific effect, since wortmannin did not inhibit recombinant activated murine ERK2 MAP kinase, protein kinase C, Raf-1, or MAP kinase kinase. In cultured muscle cells, newborn calf serum (3%) activated PI 3-kinase-alpha and PI 3-kinase-gamma isoforms, ERK and p38 MAP kinases, and stimulated chemotactic cell migration. Using wortmannin and LY-294002 to inhibit PI 3-kinase activity and PD-098059 and SB-203580 to inhibit ERK and p38 MAP kinases, we established that these enzymes are functionally important for regulation of chemotactic migration of colonic myocytes.

muscarinic receptors; cell migration; mitogen-activated protein kinases; reverse transcriptase-polymerase chain reaction; phosphatidylinositol 3-kinases


This article has been cited by other articles:


Home page
 Int ImmunolHome page
H. C. Lane, A. R. Anand, and R. K. Ganju
Cbl and Akt regulate CXCL8-induced and CXCR1- and CXCR2-mediated chemotaxis
Int. Immunol., August 1, 2006; 18(8): 1315 - 1325.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. T. Gerthoffer
Signal-Transduction Pathways that Regulate Visceral Smooth Muscle Function III. Coupling of muscarinic receptors to signaling kinases and effector proteins in gastrointestinal smooth muscles
Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G849 - G853.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
A. M. Herrera, E. C. Martinez, and C. Y. Seow
Electron microscopic study of actin polymerization in airway smooth muscle
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1161 - L1168.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
S. B. Patil, M. D. Pawar, and K. N. Bitar
Phosphorylated HSP27 essential for acetylcholine-induced association of RhoA with PKC{alpha}
Am J Physiol Gastrointest Liver Physiol, April 1, 2004; 286(4): G635 - G644.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
X. Su, E. M. Smolock, K. N. Marcel, and R. S. Moreland
Phosphatidylinositol 3-kinase modulates vascular smooth muscle contraction by calcium and myosin light chain phosphorylation-independent and -dependent pathways
Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H657 - H666.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J. Huang and C. D. Kontos
Inhibition of Vascular Smooth Muscle Cell Proliferation, Migration, and Survival by the Tumor Suppressor Protein PTEN
Arterioscler. Thromb. Vasc. Biol., May 1, 2002; 22(5): 745 - 751.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
H. Kimura, K. Sasaki, T. Meguro, and J. H. Zhang
Phosphatidylinositol 3-Kinase Inhibitor Failed to Reduce Cerebral Vasospasm in Dog Model of Experimental Subarachnoid Hemorrhage
Stroke, February 1, 2002; 33(2): 593 - 599.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. P. Allen, D. A. Linseman, H. Udo, M. Xu, J. B. Schaack, B. Varnum, E. R. Kandel, K. A. Heidenreich, and M. E. Wierman
Novel Mechanism for Gonadotropin-Releasing Hormone Neuronal Migration Involving Gas6/Ark Signaling to p38 Mitogen-Activated Protein Kinase
Mol. Cell. Biol., January 15, 2002; 22(2): 599 - 613.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. N. Bitar, A. Ibitayo, and S. B. Patil
HSP27 modulates agonist-induced association of translocated RhoA and PKC-alpha in muscle cells of the colon
J Appl Physiol, January 1, 2002; 92(1): 41 - 49.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. J. Janssen, J. Wattie, H. Lu-Chao, and T. Tazzeo
Muscarinic excitation-contraction coupling mechanisms in tracheal and bronchial smooth muscles
J Appl Physiol, September 1, 2001; 91(3): 1142 - 1151.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. T. Gerthoffer and S. J. Gunst
Signal Transduction in Smooth Muscle: Invited Review: Focal adhesion and small heat shock proteins in the regulation of actin remodeling and contractility in smooth muscle
J Appl Physiol, August 1, 2001; 91(2): 963 - 972.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Qi, R. W. Mitchell, T. Burdyga, L. E. Ford, K.-H. Kuo, and C. Y. Seow
Myosin light chain phosphorylation facilitates in vivo myosin filament reassembly after mechanical perturbation
Am J Physiol Cell Physiol, June 1, 2002; 282(6): C1298 - C1305.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online