Am J Physiol Cell Physiol AJP: Gastrointestinal and Liver Physiology
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Am J Physiol Cell Physiol 279: C319-C325, 2000;
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Vol. 279, Issue 2, C319-C325, August 2000

Low extracellular Ca2+ activates a transient Clminus current in chicken ovarian granulosa cells

Wuxuan Qin1, Stanley G. Rane2, and Elikplimi K. Asem1

1 Department of Basic Medical Sciences, School of Veterinary Medicine and 2 Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1246

The effects of low Ca2+ on ion currents in hen ovarian granulosa cells were examined. A fast activating and inactivating transient outward current (TOC) and a slowly activating outward current (SOC) could be observed. In the presence of normal Ca2+ concentration (2.5 mM) and with a holding potential of -80 mV, SOC was activated in all cells with command pulses more positive than -20 mV. In 2.5 mM Ca2+, TOC appeared in 10% of cells at the command pulse of +80 mV and in 60-85% of cells at +100 to +120 mV. In low-Ca2+ solution and command potential of +80 mV (holding potential of -80 mV), the amplitude of TOC was enhanced in cells that expressed it in normal Ca2+, and TOC appeared in 43% of the cells that did not express it initially in normal Ca2+. At both normal and low Ca2+ levels, TOC decreased as the holding potential became more positive. TOC was reduced in Cl--deficient solution and in the presence of 5-nitro-2-(3-phenylpropylamino)benzoic acid, a Cl- channel blocker. These findings suggest that chicken granulosa cells express a Ca2+-inactivated TOC carried by Cl-. This current may serve as a signal for some of the reduced metabolic functions of granulosa cells associated with Ca2+ deficiency.

patch clamp; ion channel; calcium-inactivated chloride current; ovary; ovarian follicle


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