Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 279: C213-C224, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vicini, P.
Right arrow Articles by Kushmerick, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vicini, P.
Right arrow Articles by Kushmerick, M. J.
Vol. 279, Issue 1, C213-C224, July 2000

Cellular energetics analysis by a mathematical model of energy balance: estimation of parameters in human skeletal muscle

Paolo Vicini1 and Martin J. Kushmerick1,2,3

Departments of 1 Bioengineering, 2 Radiology, and 3 Physiology and Biophysics, University of Washington, Seattle, Washington 98195

Cellular energy balance requires that the physiological demands by ATP-utilizing functions be matched by ATP synthesis to sustain muscle activity. We devised a new method of analysis of these processes in data from single individuals. Our approach is based on the logic of current information on the major mechanisms involved in this energy balance and can quantify not directly measurable parameters that govern those mechanisms. We use a mathematical model that simulates by ordinary, nonlinear differential equations three components of cellular bioenergetics (cellular ATP flux, mitochondrial oxidative phosphorylation, and creatine kinase kinetics). We incorporate data under resting conditions, during the transition toward a steady state of stimulation and during the transition during recovery back to the original resting state. Making use of prior information about the kinetic parameters, we fitted the model to previously published dynamic phosphocreatine (PCr) and inorganic phosphate (Pi) data obtained in normal subjects with an activity-recovery protocol using 31P nuclear magnetic resonance spectroscopy. The experiment consisted of a baseline phase, an ischemic phase (during which muscle stimulation and PCr utilization occurred), and an aerobic recovery phase. The model described satisfactorily the kinetics of the changes in PCr and Pi and allowed estimation of the maximal velocity of oxidative phosphorylation and of the net ATP flux in individuals both at rest and during stimulation. This work lays the foundation for a quantitative, model-based approach to the study of in vivo muscle energy balance in intact muscle systems, including human muscle.

adenosine triphosphatase; ischemia; creatine kinase; oxidative phosphorylation; skeletal muscle; SAAM II; parameter estimation


This article has been cited by other articles:


Home page
 J. Physiol.Home page
F. Wu, E. Y. Zhang, J. Zhang, R. J. Bache, and D. A. Beard
Phosphate metabolite concentrations and ATP hydrolysis potential in normal and ischaemic hearts
J. Physiol., September 1, 2008; 586(17): 4193 - 4208.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. Lai, G. M. Saidel, B. Grassi, L. B. Gladden, and M. E. Cabrera
Model of oxygen transport and metabolism predicts effect of hyperoxia on canine muscle oxygen uptake dynamics
J Appl Physiol, October 1, 2007; 103(4): 1366 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
F. Wu, J. A. L. Jeneson, and D. A. Beard
Oxidative ATP synthesis in skeletal muscle is controlled by substrate feedback
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C115 - C124.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
K. M. Hardy, B. R. Locke, M. Da Silva, and S. T. Kinsey
A reaction-diffusion analysis of energetics in large muscle fibers secondarily evolved for aerobic locomotor function
J. Exp. Biol., September 15, 2006; 209(18): 3610 - 3620.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
S. T. Kinsey, P. Pathi, K. M. Hardy, A. Jordan, and B. R. Locke
Does intracellular metabolite diffusion limit post-contractile recovery in burst locomotor muscle?
J. Exp. Biol., July 15, 2005; 208(14): 2641 - 2652.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online