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Am J Physiol Cell Physiol 279: C21-C30, 2000;
0363-6143/00 $5.00
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Vol. 279, Issue 1, C21-C30, July 2000

H2O2-mediated permeability: role of MAPK and occludin

Christopher G. Kevil, Tadayuki Oshima, Brett Alexander, Laura L. Coe, and J. Steven Alexander

Department of Molecular and Cellular Physiology, Louisiana State University Medical Center Shreveport, 1501 Kings Highway, Shreveport, Louisiana 71130

H2O2-mediated elevation in endothelial solute permeability is associated with pathological events such as ischemia-reperfusion and inflammation. To understand how H2O2 mediates increased permeability, we investigated the effects of H2O2 administration on vascular endothelial barrier properties and tight junction organization and function. We report that H2O2 exposure caused an increase in endothelial solute permeability in a time-dependent manner through extracellularly regulated kinase 1 and 2 (ERK1/ERK2) signal pathways. H2O2 exposure caused the tight junctional protein occludin to be rearranged from endothelial cell-cell junctions. Occludin rearrangement involved redistribution of occludin on the cell surface and dissociation of occludin from ZO-1. Occludin also was heavily phosphorylated on serine residues upon H2O2 administration. H2O2 mediates changes in ERK1/ERK2 phosphorylation, increases endothelial solute permeability, and alters occludin localization and phosphorylation were all blocked by PD-98059, a specific mitogen-activated protein (MAP) or ERK kinase 1 inhibitor. These data strongly suggest that H2O2-mediated increased endothelial solute permeability involves the loss of endothelial tight junction integrity through increased ERK1/ERK2 activation.

oxidants; hydrogen peroxide; tight junctions; extracellularly regulated kinase 1 and 2; mitogen-activated protein kinase


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