Am J Physiol Cell Physiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 279: C173-C187, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lader, A. S.
Right arrow Articles by Cantiello, H. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lader, A. S.
Right arrow Articles by Cantiello, H. F.
Vol. 279, Issue 1, C173-C187, July 2000

cAMP activates an ATP-permeable pathway in neonatal rat cardiac myocytes

Alan S. Lader1,2, Yong-Fu Xiao1,2, Catherine R. O'Riordan3, Adriana G. Prat1,2, G. Robert Jackson Jr.1, and Horacio F. Cantiello1,2

1 Renal Unit, Massachusetts General Hospital East, Charlestown 02129; 2 Department of Medicine, Harvard Medical School, Boston 02115; and 3 Genzyme Corporation, Framingham, Massachusetts 01701

The molecular mechanisms associated with intracellular ATP release by the heart are largely unknown. In this study the luciferin-luciferase assay and patch-clamp techniques were used to characterize the pathways responsible for ATP release in neonatal rat cardiac myocytes (NRCM). Spontaneous ATP release by NRCM was significantly increased after cAMP stimulation under physiological conditions. cAMP stimulation also induced an anion-selective electrodiffusional pathway that elicited linear, diphenylamine-2-carboxylate (DPC)-inhibitable Cl- currents in either symmetrical MgCl2 or NaCl. ATP, adenosine 5'-O-(3-thiotriphosphate), and the ATP derivatives ADP and AMP, permeated this pathway; however, GTP did not. The cAMP-induced ATP currents were inhibited by DPC and glibenclamide and by a monoclonal antibody raised against the R domain of the cystic fibrosis transmembrane conductance regulator (CFTR). The channel-like nature of the cAMP-induced ATP-permeable pathway was also determined by assessing protein kinase A-activated single channel Cl- and ATP currents in excised inside-out patches of NRCM. Single channel currents were inhibited by DPC and the anti-CFTR R domain antibody. Thus the data in this report demonstrate the presence of a cAMP-inducible electrodiffusional ATP transport mechanism in NRCM. Based on the pharmacology, patch-clamping data, and luminometry studies, the data are most consistent with the role of a functional CFTR as the anion channel implicated in cAMP-activated ATP transport in NRCM.

ATP channels; ATP release; cystic fibrosis transmembrane conductance regulator


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
R. Nandigama, M. Padmasekar, M. Wartenberg, and H. Sauer
Feed Forward Cycle of Hypotonic Stress-induced ATP Release, Purinergic Receptor Activation, and Growth Stimulation of Prostate Cancer Cells
J. Biol. Chem., March 3, 2006; 281(9): 5686 - 5693.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. A. Assef, A. E. Damiano, E. Zotta, C. Ibarra, and B. A. Kotsias
CFTR in K562 human leukemic cells
Am J Physiol Cell Physiol, August 1, 2003; 285(2): C480 - C488.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. K Dutta, Y. Okada, and R. Z Sabirov
Regulation of an ATP-conductive large-conductance anion channel and swelling-induced ATP release by arachidonic acid
J. Physiol., August 1, 2002; 542(3): 803 - 816.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. H Mitchell
Release of ATP by a human retinal pigment epithelial cell line: potential for autocrine stimulation through subretinal space
J. Physiol., July 1, 2001; 534(1): 193 - 202.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online