Am J Physiol Cell Physiol Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Am J Physiol Cell Physiol 278: C1088-C1098, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chase, P. B.
Right arrow Articles by Daniel, T. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chase, P. B.
Right arrow Articles by Daniel, T. L.
Vol. 278, Issue 6, C1088-C1098, June 2000

Viscosity and solute dependence of F-actin translocation by rabbit skeletal heavy meromyosin

P. Bryant Chase1,2, Ying Chen1, Kristi L. Kulin3, and Thomas L. Daniel3

Departments of 1 Radiology, 2 Physiology and Biophysics, and 3 Zoology, University of Washington, Seattle, Washington 98195

We tested the hypothesis that solvent viscosity affects translocation of rhodamine phalloidin-labeled F-actin by rabbit skeletal heavy meromyosin (HMM). When viscosity was increased using either glycerol, fructose, sucrose, or dextran (1.5, 6.0, or 15-20 kDa mol mass), there was little or no effect on the fraction of moving filaments, whereas sliding speed decreased in inverse proportion to viscosity. The results could be explained neither by an effect of osmotic pressure at high solute concentrations nor by altered solvent drag on the actin filament. Elevated viscosity inhibited HMM ATPase activity in solution, but only at much higher viscosities than were needed to reduce sliding speed. Polyethylene glycols (300, 1,000, or 3,000 mol wt) also inhibited speed via elevated viscosity but secondarily inhibited by enhancing electrostatic interactions. These results demonstrate that a diffusion-controlled process intrinsic to cross-bridge cycling can be limiting to actomyosin function.

in vitro motility assay; protein dynamics of biological motors; diffusion; sugars; polyethylene glycols


This article has been cited by other articles:


Home page
J. Physiol.Home page
B. Schoffstall, N. M. Brunet, S. Williams, V. F. Miller, A. T. Barnes, F. Wang, L. A. Compton, L. A. McFadden, D. W. Taylor, M. Seavy, et al.
Ca2+ sensitivity of regulated cardiac thin filament sliding does not depend on myosin isoform
J. Physiol., December 15, 2006; 577(3): 935 - 944.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
B. Schoffstall, A. Clark, and P. B. Chase
Positive Inotropic Effects of Low dATP/ATP Ratios on Mechanics and Kinetics of Porcine Cardiac Muscle
Biophys. J., September 15, 2006; 91(6): 2216 - 2226.
[Abstract] [Full Text] [PDF]


Home page
J. Physiol.Home page
E. W. Clemmens, M. Entezari, D. A Martyn, and M. Regnier
Different effects of cardiac versus skeletal muscle regulatory proteins on in vitro measures of actin filament speed and force
J. Physiol., August 1, 2005; 566(3): 737 - 746.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
B. Schoffstall, A. Kataoka, A. Clark, and P. B. Chase
Effects of Rapamycin on Cardiac and Skeletal Muscle Contraction and Crossbridge Cycling
J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 12 - 18.
[Abstract] [Full Text] [PDF]


Home page
Biophys. JHome page
B. Liang, Y. Chen, C.-K. Wang, Z. Luo, M. Regnier, A. M. Gordon, and P. B. Chase
Ca2+ Regulation of Rabbit Skeletal Muscle Thin Filament Sliding: Role of Cross-Bridge Number
Biophys. J., September 1, 2003; 85(3): 1775 - 1786.
[Abstract] [Full Text] [PDF]


Home page
Physiol. GenomicsHome page
J. Kohler, Y. Chen, B. Brenner, A. M. Gordon, T. Kraft, D. A. Martyn, M. Regnier, A. J. Rivera, C.-K. Wang, and P. B. Chase
Familial hypertrophic cardiomyopathy mutations in troponin I (K183{Delta}, G203S, K206Q) enhance filament sliding
Physiol Genomics, July 7, 2003; 14(2): 117 - 128.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online