Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 278: C885-C894, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bertog, M.
Right arrow Articles by Harris, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertog, M.
Right arrow Articles by Harris, A.
Vol. 278, Issue 5, C885-C894, May 2000

Ovine male genital duct epithelial cells differentiate in vitro and express functional CFTR and ENaC

Marko Bertog2,*, David J. Smith1,*, Andreas Bielfeld-Ackermann2, John Bassett3, David J. P. Ferguson4, Christoph Korbmacher2, and Ann Harris1

1 Paediatric Molecular Genetics, Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, Oxford OX3 9DS; 2 University Laboratory of Physiology, Oxford OX1 3PT; 3 Growth and Development Unit, Field Laboratory, Oxford University, Oxford OX2 8QJ; and 4 Department of Pathology, Oxford University, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom

To investigate the biology of the male genital duct epithelium, we have established cell cultures from the ovine vas deferens and epididymis epithelium. These cells develop tight junctions, high transepithelial electrical resistance, and a lumen-negative transepithelial potential difference as a sign of active transepithelial ion transport. In epididymis cultures the equivalent short-circuit current (Isc) averaged 20.8 ± 0.7 µA/cm2 (n = 150) and was partially inhibited by apical application of amiloride with an inhibitor concentration of 0.64 µM. In vas deferens cultures, Isc averaged 14.4 ± 1.1 µA/cm2 (n = 18) and was also inhibited by apical application of amiloride with a half-maximal inhibitor concentration (Ki) of 0.68 µM. The remaining amiloride-insensitive Isc component in epididymis and vas deferens cells was partially inhibited by apical application of the Cl- channel blocker diphenylamine-2-carboxylic acid (1 mM). It was largely dependent on extracellular Cl- and, to a lesser extent, on extracellular HCO-3. It was further stimulated by basolateral application of forskolin (10-5 M), which increased Isc by 3.1 ± 0.3 µA/cm2 (n =65) in epididymis and 0.9 ± 0.1 µA/cm2 (n = 11) in vas deferens. These findings suggest that cultured ovine vas deferens and epididymis cells absorb Na+ via amiloride-sensitive epithelial Na+ channels (ENaC) and secrete Cl- and HCO-3 via apical cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channels. This interpretation is supported by RT-PCR data showing that vas deferens and epididymis cells express CFTR and ENaC mRNA.

ovine genital ducts; cystic fibrosis transmembrane conductance regulator; epithelial sodium channel

* M. Bertog and D. J. Smith contributed equally to this work.




This article has been cited by other articles:


Home page
 J. Physiol.Home page
M. Bertog, J. E. Cuffe, S. Pradervand, E. Hummler, A. Hartner, M. Porst, K. F. Hilgers, B. C. Rossier, and C. Korbmacher
Aldosterone responsiveness of the epithelial sodium channel (ENaC) in colon is increased in a mouse model for Liddle's syndrome
J. Physiol., January 15, 2008; 586(2): 459 - 475.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. W. Carlin, R. L. Sedlacek, R. R. Quesnell, F. Pierucci-Alves, D. M. Grieger, and B. D. Schultz
PVD9902, a porcine vas deferens epithelial cell line that exhibits neurotransmitter-stimulated anion secretion and expresses numerous HCO3- transporters
Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1560 - C1571.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-L. Ji and D. J. Benos
Degenerin Sites Mediate Proton Activation of {delta}{beta}{gamma}-Epithelial Sodium Channel
J. Biol. Chem., June 25, 2004; 279(26): 26939 - 26947.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. W. Carlin, J. H. Lee, D. C. Marcus, and B. D. Schultz
Adenosine Stimulates Anion Secretion Across Cultured and Native Adult Human Vas Deferens Epithelia
Biol Reprod, March 1, 2003; 68(3): 1027 - 1034.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. W. Carlin, R. R. Quesnell, L. Zheng, K. E. Mitchell, and B. D. Schultz
Functional and molecular evidence for Na+-HCO3- cotransporter in porcine vas deferens epithelia
Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1033 - C1044.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
R. L. Sedlacek, R. W. Carlin, A. K. Singh, and B. D. Schultz
Neurotransmitter-stimulated ion transport by cultured porcine vas deferens epithelium
Am J Physiol Renal Physiol, September 1, 2001; 281(3): F557 - F570.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online