Am J Physiol Cell Physiol Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 278: C517-C525, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (14)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dutka, T. L.
Right arrow Articles by Lamb, G. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dutka, T. L.
Right arrow Articles by Lamb, G. D.
Vol. 278, Issue 3, C517-C525, March 2000

Effect of lactate on depolarization-induced Ca2+ release in mechanically skinned skeletal muscle fibers

T. L. Dutka and G. D. Lamb

Department of Zoology, La Trobe University, Bundoora, Victoria, 3083 Australia

It is unclear whether accumulation of lactate in skeletal muscle fibers during intense activity contributes to muscle fatigue. Using mechanically skinned fibers from rat and toad muscle, we were able to examine the effect of L(+)-lactate on excitation-contraction coupling independently of other metabolic changes. We investigated the effects of lactate on the contractile apparatus, caffeine-induced Ca2+ release from the sarcoplasmic reticulum, and depolarization-induced Ca2+ release. Lactate (15 or 30 mM) had only a small inhibitory effect directly on the contractile apparatus and caused appreciable (20-35%) inhibition of caffeine-induced Ca2+ release, seemingly by a direct effect on the Ca2+ release channels. However, 15 mM lactate had no detectable effect on Ca2+ release when it was triggered by the normal voltage sensor mechanism, and 30 mM lactate reduced such release by only <10%. These results indicate that lactate has only a relatively small inhibitory effect on normal excitation-contraction coupling, indicating that lactate accumulation per se is not a major factor in muscle fatigue.

excitation-contraction coupling; fatigue; exercise; calcium release channel; ryanodine receptor


This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
D. G. Allen, G. D. Lamb, and H. Westerblad
Skeletal Muscle Fatigue: Cellular Mechanisms
Physiol Rev, January 1, 2008; 88(1): 287 - 332.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. A. Usher-Smith, J. A. Fraser, P. S. J. Bailey, J. L. Griffin, and C. L.-H. Huang
The influence of intracellular lactate and H+ on cell volume in amphibian skeletal muscle
J. Physiol., June 15, 2006; 573(3): 799 - 818.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
A. D. Karelis, M. Marcil, F. Peronnet, and P. F. Gardiner
Effect of lactate infusion on M-wave characteristics and force in the rat plantaris muscle during repeated stimulation in situ
J Appl Physiol, June 1, 2004; 96(6): 2133 - 2138.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Goodman, M. Patterson, and G. Stephenson
MHC-based fiber type and E-C coupling characteristics in mechanically skinned muscle fibers of the rat
Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1448 - C1459.
[Abstract] [Full Text] [PDF]

Home page
 ptjournalHome page
S. K Stackhouse, D. S Reisman, and S. A Binder-Macleod
Challenging the Role of pH in Skeletal Muscle Fatigue
Physical Therapy, December 1, 2001; 81(12): 1897 - 1903.
[Full Text] [PDF]

Home page
 J. Physiol.Home page
J. Lannergren, J. D Bruton, and H. Westerblad
Vacuole formation in fatigued skeletal muscle fibres from frog and mouse: effects of extracellular lactate
J. Physiol., August 1, 2000; 526(3): 597 - 611.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online