Am J Physiol Cell Physiol Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 278: C463-C472, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (9)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Oprins, J. C. J.
Right arrow Articles by Groot, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oprins, J. C. J.
Right arrow Articles by Groot, J. A.
Vol. 278, Issue 3, C463-C472, March 2000

TNF-alpha potentiates the ion secretion induced by muscarinic receptor activation in HT29cl.19A cells

Judith C. J. Oprins, Helen P. Meijer, and Jack A. Groot

Institute for Neurobiology, Biological Faculty, University of Amsterdam, 1098 SM Amsterdam, The Netherlands

Chronic gastrointestinal diseases such as ulcerative colitis and Crohn's disease are characterized by severe diarrhea. Mucosal biopsies of these patients show enhanced levels of cytokines, secreted by infiltrated inflammatory cells. In this study, we investigated the effect of the cytokine tumor necrosis factor-alpha (TNF-alpha ) on ion secretion in human intestinal epithelial cells. The conventional microelectrode technique in the cell line HT29cl.19A was used, which allows for simultaneous measurements of transepithelial potential difference and intracellular potential difference across the apical membrane. Preincubation (2-78 h) with 10 ng/ml TNF-alpha did not change basal secretory activity. However, the secretory response to the muscarinic receptor agonist carbachol was strongly increased after exposure to TNF-alpha . Application of the protein kinase C (PKC) inhibitor GF 109203X (bisindolylmaleimide I) inhibited the response to carbachol as well as the TNF-alpha -potentiated response, indicating that PKC mediates the effect of carbachol in this cell line. Propranolol, a substance that inhibits the phospholipase D (PLD) pathway, strongly reduced the response to muscarinic stimulation and its potentiation by TNF-alpha . The results indicate that activation of PLD is involved in ion secretion induced by muscarinic receptor activation and that TNF-alpha can potentiate this pathway.

phospholipase D; protein kinase C; intestinal epithelia; carbachol; cytokine; tumor necrosis factor-alpha


This article has been cited by other articles:


Home page
 CVIHome page
B Jiang, L. Snipes-Magaldi, P. Dennehy, H. Keyserling, R. C. Holman, J. Bresee, J. Gentsch, and R. I. Glass
Cytokines as Mediators for or Effectors against Rotavirus Disease in Children
Clin. Vaccine Immunol., November 1, 2003; 10(6): 995 - 1001.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
J C J Oprins, C van der Burg, H P Meijer, T Munnik, and J A Groot
Tumour necrosis factor {alpha} potentiates ion secretion induced by histamine in a human intestinal epithelial cell line and in mouse colon: involvement of the phospholipase D pathway
Gut, March 1, 2002; 50(3): 314 - 321.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. C. J. Oprins, C. van der Burg, H. P. Meijer, T. Munnik, and J. A. Groot
PLD pathway involved in carbachol-induced Cl{-} secretion: possible role of TNF-{alpha}
Am J Physiol Cell Physiol, April 1, 2001; 280(4): C789 - C795.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Zareie, P. K. Singh, E. J. Irvine, P. M. Sherman, D. M. McKay, and M. H. Perdue
Monocyte/Macrophage Activation by Normal Bacteria and Bacterial Products : Implications for Altered Epithelial Function in Crohn's Disease
Am. J. Pathol., March 1, 2001; 158(3): 1101 - 1109.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online