| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Musculoskeletal Research Laboratory, Departments of Orthopedics and Rehabilitation and of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey, Pennsylvania 17033
Gap junctional channels
facilitate intercellular communication and in doing so may
contribute to cellular differentiation. To test this hypothesis, we
examined gap junction expression and function in a
temperature-sensitive human fetal osteoblastic cell line (hFOB 1.19)
that when cultured at 37°C proliferates rapidly but when cultured
at 39.5°C proliferates slowly and displays increased alkaline
phosphatase activity and osteocalcin synthesis. We found that hFOB 1.19 cells express abundant connexin 43 (Cx43) protein and mRNA. In
contrast, Cx45 mRNA was expressed to a lesser degree, and Cx26 and Cx32
mRNA were not detected. Culturing hFOB 1.19 cells at 39.5°C,
relative to 37°C, inhibited proliferation, increased Cx43 mRNA and
protein expression, and increased gap junctional intercellular
communication (GJIC). Blocking GJIC with 18
-glycyrrhetinic acid prevented the increase in alkaline phosphatase
activity resulting from culture at 39.5°C but did not affect
osteocalcin levels. These results suggest that gap junction function
and expression parallel osteoblastic differentiation and contribute to
the expression of alkaline phosphatase activity, a marker for fully
differentiated osteoblastic cells.
connexin; alkaline phosphatase; osteocalcin; proliferation; bone
This article has been cited by other articles:
|
|
 |
|
  D. J. Chung, C. H. M. Castro, M. Watkins, J. P. Stains, M. Y. Chung, V. L. Szejnfeld, K. Willecke, M. Theis, and R. Civitelli Low peak bone mass and attenuated anabolic response to parathyroid hormone in mice with an osteoblast-specific deletion of connexin43 J. Cell Sci., October 15, 2006; 119(20): 4187 - 4198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yao, M. Kitamura, Y. Zhu, Y. Meng, A. Kasai, N. Hiramatsu, T. Morioka, M. Takeda, and T. Oite Synergistic effects of PDGF-BB and cAMP-elevating agents on expression of connexin43 in mesangial cells Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1083 - F1093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Li, Z. Zhou, M. M. Saunders, and H. J. Donahue Modulation of connexin43 alters expression of osteoblastic differentiation markers Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1248 - C1255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Thi, T. Kojima, S. C. Cowin, S. Weinbaum, and D. C. Spray Fluid shear stress remodels expression and function of junctional proteins in cultured bone cells Am J Physiol Cell Physiol, February 1, 2003; 284(2): C389 - C403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Villars, B. Guillotin, T. Amedee, S. Dutoya, L. Bordenave, R. Bareille, and J. Amedee Effect of HUVEC on human osteoprogenitor cell differentiation needs heterotypic gap junction communication Am J Physiol Cell Physiol, April 1, 2002; 282(4): C775 - C785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Saunders, J. You, J. E. Trosko, H. Yamasaki, Z. Li, H. J. Donahue, and C. R. Jacobs Gap junctions and fluid flow response in MC3T3-E1 cells Am J Physiol Cell Physiol, December 1, 2001; 281(6): C1917 - C1925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Saunders, M. J. Seraj, Z. Li, Z. Zhou, C. R. Winter, D. R. Welch, and H. J. Donahue Breast Cancer Metastatic Potential Correlates with a Breakdown in Homospecific and Heterospecific Gap Junctional Intercellular Communication Cancer Res., March 1, 2001; 61(5): 1765 - 1767. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
