Am J Physiol Cell Physiol Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 278: C207-C211, 2000;
0363-6143/00 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (75)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scott, D. A.
Right arrow Articles by Karniski, L. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scott, D. A.
Right arrow Articles by Karniski, L. P.
Vol. 278, Issue 1, C207-C211, January 2000

RAPID COMMUNICATION
Human pendrin expressed in Xenopus laevis oocytes mediates chloride/formate exchange

Daryl A. Scott1 and Lawrence P. Karniski2

1 Division of Medical Genetics, Department of Pediatrics, and 2 Department of Internal Medicine, Veterans Affairs Medical Center, University of Iowa College of Medicine, Iowa City, Iowa 52242

Pendred syndrome, characterized by congenital sensorineural hearing loss and goiter, is one of the most common forms of syndromic deafness. The gene causing Pendred syndrome (PDS) encodes a protein designated pendrin, which is expressed in the thyroid, kidney, and fetal cochlea. Pendrin functions as an iodide and chloride transporter, but its role in the development of hearing loss and goiter is unknown. In this study, we examined the mechanism of pendrin-mediated anion transport in Xenopus laevis oocytes. Unlabeled formate added to the uptake medium inhibited pendrin-mediated 36Cl uptake in X. laevis oocytes. In addition, the uptake of [14C]formate was stimulated in oocytes injected with PDS cRNA compared with water-injected controls. These results indicate that formate is a substrate for pendrin. Furthermore, chloride stimulated the efflux of [14C]formate and formate stimulated the efflux of 36Cl in oocytes expressing pendrin, results consistent with pendrin-mediated chloride/formate exchange. These data demonstrate that pendrin is functionally similar to the renal chloride/formate exchanger, which serves as an important mechanism of chloride transport in the proximal tubule. A similar process could participate in the development of ion gradients within the inner ear.

Pendred syndrome; pendrin; chloride; formate


This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
P. Wangemann, H.-M. Kim, S. Billings, K. Nakaya, X. Li, R. Singh, D. S. Sharlin, D. Forrest, D. C. Marcus, and P. Fong
Developmental delays consistent with cochlear hypothyroidism contribute to failure to develop hearing in mice lacking Slc26a4/pendrin expression
Am J Physiol Renal Physiol, November 1, 2009; 297(5): F1435 - F1447.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y. H. Kim, T. D. Pham, W. Zheng, S. Hong, C. Baylis, V. Pech, W. H. Beierwaltes, D. B. Farley, L. E. Braverman, J. W. Verlander, et al.
Role of pendrin in iodide balance: going with the flow
Am J Physiol Renal Physiol, October 1, 2009; 297(4): F1069 - F1079.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Pera, S. Dossena, S. Rodighiero, M. Gandia, G. Botta, G. Meyer, F. Moreno, C. Nofziger, C. Hernandez-Chico, and M. Paulmichl
Functional assessment of allelic variants in the SLC26A4 gene involved in Pendred syndrome and nonsyndromic EVA
PNAS, November 25, 2008; 105(47): 18608 - 18613.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Adler, E. Efrati, and I. Zelikovic
Molecular mechanisms of epithelial cell-specific expression and regulation of the human anion exchanger (pendrin) gene
Am J Physiol Cell Physiol, May 1, 2008; 294(5): C1261 - C1276.
[Abstract] [Full Text] [PDF]

Home page
 Arch Otolaryngol Head Neck SurgHome page
Z. N. Brownstein, A. A. Dror, D. Gilony, L. Migirov, K. Hirschberg, and K. B. Avraham
A Novel SLC26A4 (PDS) Deafness Mutation Retained in the Endoplasmic Reticulum
Arch Otolaryngol Head Neck Surg, April 1, 2008; 134(4): 403 - 407.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
K. Nakaya, D. G. Harbidge, P. Wangemann, B. D. Schultz, E. D. Green, S. M. Wall, and D. C. Marcus
Lack of pendrin HCO3- transport elevates vestibular endolymphatic [Ca2+] by inhibition of acid-sensitive TRPV5 and TRPV6 channels
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1314 - F1321.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. Wangemann, K. Nakaya, T. Wu, R. J. Maganti, E. M. Itza, J. D. Sanneman, D. G. Harbidge, S. Billings, and D. C. Marcus
Loss of cochlear HCO3- secretion causes deafness via endolymphatic acidification and inhibition of Ca2+ reabsorption in a Pendred syndrome mouse model
Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1345 - F1353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
J. W. Verlander, Y. H. Kim, W. Shin, T. D. Pham, K. A. Hassell, W. H. Beierwaltes, E. D. Green, L. Everett, S. W. Matthews, and S. M. Wall
Dietary Cl- restriction upregulates pendrin expression within the apical plasma membrane of type B intercalated cells
Am J Physiol Renal Physiol, October 1, 2006; 291(4): F833 - F839.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F. Katoh, M. Tresguerres, K. M. Lee, T. Kaneko, K. Aida, and G. G. Goss
Cloning of rainbow trout SLC26A1: involvement in renal sulfate secretion
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1468 - R1478.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y.-H. Kim, J. W. Verlander, S. W. Matthews, I. Kurtz, W. Shin, I. D. Weiner, L. A. Everett, E. D. Green, S. Nielsen, and S. M. Wall
Intercalated cell H+/OH- transporter expression is reduced in Slc26a4 null mice
Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1262 - F1272.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Dentice, C. Luongo, A. Elefante, R. Ambrosio, S. Salzano, M. Zannini, R. Nitsch, R. Di Lauro, G. Rossi, G. Fenzi, et al.
Pendrin Is a Novel In Vivo Downstream Target Gene of the TTF-1/Nkx-2.1 Homeodomain Transcription Factor in Differentiated Thyroid Cells
Mol. Cell. Biol., November 15, 2005; 25(22): 10171 - 10182.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
F. Quentin, R. Chambrey, M. M. Trinh-Trang-Tan, M. Fysekidis, M. Cambillau, M. Paillard, P. S. Aronson, and D. Eladari
The Cl-/HCO3- exchanger pendrin in the rat kidney is regulated in response to chronic alterations in chloride balance
Am J Physiol Renal Physiol, December 1, 2004; 287(6): F1179 - F1188.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. P. Gillam, A. R. Sidhaye, E. J. Lee, J. Rutishauser, C. W. Stephan, and P. Kopp
Functional Characterization of Pendrin in a Polarized Cell System: EVIDENCE FOR PENDRIN-MEDIATED APICAL IODIDE EFFLUX
J. Biol. Chem., March 26, 2004; 279(13): 13004 - 13010.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. N Chernova, L. Jiang, B. E Shmukler, C. W Schweinfest, P. Blanco, S. D Freedman, A. K Stewart, and S. L Alper
Acute regulation of the SLC26A3 congenital chloride diarrhoea anion exchanger (DRA) expressed in Xenopus oocytes
J. Physiol., May 15, 2003; 549(1): 3 - 19.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
H. Lohi, G. Lamprecht, D. Markovich, A. Heil, M. Kujala, U. Seidler, and J. Kere
Isoforms of SLC26A6 mediate anion transport and have functional PDZ interaction domains
Am J Physiol Cell Physiol, March 1, 2003; 284(3): C769 - C779.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. M. Wall, K. A. Hassell, I. E. Royaux, E. D. Green, J. Y. Chang, G. L. Shipley, and J. W. Verlander
Localization of pendrin in mouse kidney
Am J Physiol Renal Physiol, January 1, 2003; 284(1): F229 - F241.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
L. P. Karniski, T. Wang, L. A. Everett, E. D. Green, G. Giebisch, and P. S. Aronson
Formate-stimulated NaCl absorption in the proximal tubule is independent of the pendrin protein
Am J Physiol Renal Physiol, November 1, 2002; 283(5): F952 - F956.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. M. Piermarini, J. W. Verlander, I. E. Royaux, and D. H. Evans
Pendrin immunoreactivity in the gill epithelium of a euryhaline elasmobranch
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2002; 283(4): R983 - R992.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Y.-H. Kim, T.-H. Kwon, S. Frische, J. Kim, C. C. Tisher, K. M. Madsen, and S. Nielsen
Immunocytochemical localization of pendrin in intercalated cell subtypes in rat and mouse kidney
Am J Physiol Renal Physiol, October 1, 2002; 283(4): F744 - F754.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
Q. Xie, R. Welch, A. Mercado, M. F. Romero, and D. B. Mount
Molecular characterization of the murine Slc26a6 anion exchanger: functional comparison with Slc26a1
Am J Physiol Renal Physiol, October 1, 2002; 283(4): F826 - F838.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Lohi, M. Kujala, S. Makela, E. Lehtonen, M. Kestila, U. Saarialho-Kere, D. Markovich, and J. Kere
Functional Characterization of Three Novel Tissue-specific Anion Exchangers SLC26A7, -A8, and -A9
J. Biol. Chem., April 12, 2002; 277(16): 14246 - 14254.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Markovich
Physiological Roles and Regulation of Mammalian Sulfate Transporters
Physiol Rev, October 1, 2001; 81(4): 1499 - 1533.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. Cauvi, M.-C. Nlend, N. Venot, and O. Chabaud
Sulfate transport in porcine thyroid cells. Effects of thyrotropin and iodide
Am J Physiol Endocrinol Metab, September 1, 2001; 281(3): E440 - E448.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S. Haila, J. Hastbacka, T. Bohling, M.-L. Karjalainen-Lindsberg, J. Kere, and U. Saarialho-Kere
SLC26A2 (Diastrophic Dysplasia Sulfate Transporter) Is Expressed in Developing and Mature Cartilage But Also in Other Tissues and Cell Types
J. Histochem. Cytochem., August 1, 2001; 49(8): 973 - 982.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. Oliver, D. Z. Z. He, N. Klocker, J. Ludwig, U. Schulte, S. Waldegger, J. P. Ruppersberg, P. Dallos, and B. Fakler
Intracellular Anions as the Voltage Sensor of Prestin, the Outer Hair Cell Motor Protein
Science, June 22, 2001; 292(5525): 2340 - 2343.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
S. A. KARUMANCHI, L. JIANG, B. KNEBELMANN, A. K. STUART-TILLEY, S. L. ALPER, and V. P. SUKHATME
VHL tumor suppressor regulates Cl-/HCO3- exchange and Na+/H+ exchange activities in renal carcinoma cells
Physiol Genomics, April 2, 2001; 5(3): 119 - 128.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. Soleimani, T. Greeley, S. Petrovic, Z. Wang, H. Amlal, P. Kopp, and C. E. Burnham
Pendrin: an apical Cl{-}/OH{-}/HCO3{-} exchanger in the kidney cortex
Am J Physiol Renal Physiol, February 1, 2001; 280(2): F356 - F364.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
L. A. Everett, I. A. Belyantseva, K. Noben-Trauth, R. Cantos, A. Chen, S. I. Thakkar, S. L. Hoogstraten-Miller, B. Kachar, D. K. Wu, and E. D. Green
Targeted disruption of mouse Pds provides insight about the inner-ear defects encountered in Pendred syndrome
Hum. Mol. Genet., January 1, 2001; 10(2): 153 - 161.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
D. A. Scott, R. Wang, T. M. Kreman, M. Andrews, J. M. McDonald, J. R. Bishop, R. J.H. Smith, L. P. Karniski, and V. C. Sheffield
Functional differences of the PDS gene product are associated with phenotypic variation in patients with Pendred syndrome and non-syndromic hearing loss (DFNB4)
Hum. Mol. Genet., July 1, 2000; 9(11): 1709 - 1715.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. Cuppoletti
An ion transporter involved in congenital deafness Focus on "Human pendrin expressed in Xenopus laevis oocytes mediates chloride/formate exchange"
Am J Physiol Cell Physiol, January 1, 2000; 278(1): C11 - C12.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online