| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201
In many nonexcitable cells, hormones and neurotransmitters activate Na+ influx and mobilize Ca2+ from intracellular stores. The stores are replenished by Ca2+ influx via "store-operated" Ca2+ channels (SOC). The main routes of Na+ entry in these cells are unresolved, and no role for Na+ in signaling has been recognized. We demonstrate that the SOC are a major Na+ entry route in arterial myocytes. Unloading of the Ca2+ stores with cyclopiazonic acid (a sarcoplasmic reticulum Ca2+ pump inhibitor) and caffeine induces a large external Na+-dependent rise in the cytosolic Na+ concentration. One component of this rise in cytosolic Na+ concentration is likely due to Na+/Ca2+ exchange; it depends on elevation of cytosolic Ca2+ and is insensitive to 10 mM Mg2+ and 10 µM La3+. Another component is inhibited by Mg2+ and La3+, blockers of SOC; this component persists in cells preloaded with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid to buffer Ca2+ transients and prevent Na+/Ca2+ exchange-mediated Na+ entry. This Na+ entry apparently is mediated by SOC. The Na+ entry influences Na+ pump activity and Na+/Ca2+ exchange and has unexpectedly large effects on cell-wide Ca2+ signaling. The SOC pathway may be a general mechanism by which Na+ participates in signaling in many types of cells.
sodium-calcium exchange; magnesium; sodium pump; cytosolic sodium concentration
This article has been cited by other articles:
|
|
 |
|
  D. Poburko, C.-H. Liao, C. van Breemen, and N. Demaurex Mitochondrial Regulation of Sarcoplasmic Reticulum Ca2+ Content in Vascular Smooth Muscle Cells Circ. Res., January 2, 2009; 104(1): 104 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Cao, K. Payne, W. Lee-Kwon, Z. Zhang, S. W. Lim, J. Hamlyn, M. P. Blaustein, H. M. Kwon, and T. L. Pallone Chronic ouabain treatment induces vasa recta endothelial dysfunction in the rat Am J Physiol Renal Physiol, January 1, 2009; 296(1): F98 - F106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Raina, S. R. Ella, and M. A. Hill Decreased activity of the smooth muscle Na+/Ca2+ exchanger impairs arteriolar myogenic reactivity J. Physiol., March 15, 2008; 586(6): 1669 - 1681. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhang, C. Cao, Z. Zhang, W. G. Wier, A. Edwards, and T. L. Pallone Membrane current oscillations in descending vasa recta pericytes Am J Physiol Renal Physiol, March 1, 2008; 294(3): F656 - F666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. H. Pojoga, T. M. Yao, S. Sinha, R. L. Ross, J. C. Lin, J. D. Raffetto, G. K. Adler, G. H. Williams, and R. A. Khalil Effect of dietary sodium on vasoconstriction and eNOS-mediated vascular relaxation in caveolin-1-deficient mice Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1258 - H1265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cai, L. Wu, W. Qu, D. Malhotra, Z. Xie, J. I. Shapiro, and J. Liu Regulation of apical NHE3 trafficking by ouabain-induced activation of the basolateral Na+-K+-ATPase receptor complex Am J Physiol Cell Physiol, February 1, 2008; 294(2): C555 - C563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Blaustein and W. G. Wier Local Sodium, Global Reach: Filling the Gap Between Salt and Hypertension Circ. Res., November 9, 2007; 101(10): 959 - 961. [Full Text] [PDF]
|
|
|
|
|
|
D. Poburko, C.-H. Liao, V. S. Lemos, E. Lin, Y. Maruyama, W. C. Cole, and C. van Breemen Transient Receptor Potential Channel 6 Mediated, Localized Cytosolic [Na+] Transients Drive Na+/Ca2+ Exchanger Mediated Ca2+ Entry in Purinergically Stimulated Aorta Smooth Muscle Cells Circ. Res., November 9, 2007; 101(10): 1030 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Becker, L. M. Moir, V. A. Snetkov, and P. I. Aaronson Hypoxic pulmonary vasoconstriction in intact rat intrapulmonary arteries is not initiated by inhibition of Na+-Ca2+ exchange Am J Physiol Lung Cell Mol Physiol, October 1, 2007; 293(4): L982 - L990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. I. Clyman, N. Waleh, H. Kajino, C. Roman, and F. Mauray Calcium-dependent and calcium-sensitizing pathways in the mature and immature ductus arteriosus Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1650 - R1656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Schoner and G. Scheiner-Bobis Endogenous and exogenous cardiac glycosides: their roles in hypertension, salt metabolism, and cell growth Am J Physiol Cell Physiol, August 1, 2007; 293(2): C509 - C536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhang, H. Dong, L. J. Rubin, and J. X.-J. Yuan Upregulation of Na+/Ca2+ exchanger contributes to the enhanced Ca2+ entry in pulmonary artery smooth muscle cells from patients with idiopathic pulmonary arterial hypertension Am J Physiol Cell Physiol, June 1, 2007; 292(6): C2297 - C2305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Edwards and T. L. Pallone Modification of cytosolic calcium signaling by subplasmalemmal microdomains Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1827 - F1845. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hirota, E. Pertens, and L. J. Janssen The reverse mode of the Na+/Ca2+ exchanger provides a source of Ca2+ for store refilling following agonist-induced Ca2+ mobilization Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L438 - L447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, R. Berra-Romani, M. J. Sinnegger-Brauns, J. Striessnig, M. P. Blaustein, and D. R. Matteson Role of Cav1.2 L-type Ca2+ channels in vascular tone: effects of nifedipine and Mg2+ Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H415 - H425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pittner, K. Rhinehart, and T. L. Pallone Ouabain modulation of endothelial calcium signaling in descending vasa recta Am J Physiol Renal Physiol, October 1, 2006; 291(4): F761 - F769. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Dong, Y. Jiang, C. R. Triggle, X. Li, and J. Lytton Novel role for K+-dependent Na+/Ca2+ exchangers in regulation of cytoplasmic free Ca2+ and contractility in arterial smooth muscle Am J Physiol Heart Circ Physiol, September 1, 2006; 291(3): H1226 - H1235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Cheng, A.F. James, R.R. Foster, J.C. Hancox, and D.O. Bates VEGF Activates Receptor-Operated Cation Channels in Human Microvascular Endothelial Cells Arterioscler Thromb Vasc Biol, August 1, 2006; 26(8): 1768 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Rao, O. Platoshyn, V. A. Golovina, L. Liu, T. Zou, B. S. Marasa, D. J. Turner, J. X.-J. Yuan, and J.-Y. Wang TRPC1 functions as a store-operated Ca2+ channel in intestinal epithelial cells and regulates early mucosal restitution after wounding Am J Physiol Gastrointest Liver Physiol, April 1, 2006; 290(4): G782 - G792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Khalil Dietary salt and hypertension: new molecular targets add more spice Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2006; 290(3): R509 - R513. [Full Text] [PDF]
|
|
|
|
|
|
J. M. Dai, K.-H. Kuo, J. M. Leo, C. van Breemen, and C.-H. Lee Mechanism of ACh-induced asynchronous calcium waves and tonic contraction in porcine tracheal muscle bundle Am J Physiol Lung Cell Mol Physiol, March 1, 2006; 290(3): L459 - L469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Dong, Z. M. Sellers, A. Smith, J. Y. C. Chow, and K. E. Barrett Na+/Ca2+ exchange regulates Ca2+-dependent duodenal mucosal ion transport and HCO3- secretion in mice Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G457 - G465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhang, J. X.-J. Yuan, K. E. Barrett, and H. Dong Role of Na+/Ca2+ exchange in regulating cytosolic Ca2+ in cultured human pulmonary artery smooth muscle cells Am J Physiol Cell Physiol, February 1, 2005; 288(2): C245 - C252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Beech, K. Muraki, and R. Flemming Non-selective cationic channels of smooth muscle and the mammalian homologues of Drosophila TRP J. Physiol., September 15, 2004; 559(3): 685 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gwanyanya, B. Amuzescu, S. I. Zakharov, R. Macianskiene, K. R. Sipido, V. M. Bolotina, J. Vereecke, and K. Mubagwa Magnesium-inhibited, TRPM6/7-like channel in cardiac myocytes: permeation of divalent cations and pH-mediated regulation J. Physiol., September 15, 2004; 559(3): 761 - 776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-J. Lin, G. P.H. Leung, W.-M. Zhang, X.-R. Yang, K.-P. Yip, C.-M. Tse, and J. S.K. Sham Chronic Hypoxia-Induced Upregulation of Store-Operated and Receptor-Operated Ca2+ Channels in Pulmonary Arterial Smooth Muscle Cells: A Novel Mechanism of Hypoxic Pulmonary Hypertension Circ. Res., September 3, 2004; 95(5): 496 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. M. Bolotina Store-Operated Channels: Diversity and Activation Mechanisms Sci. Signal., July 27, 2004; 2004(243): pe34 - pe34. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wang, L. G. Laurier, S. M. Sims, and H. G. Preiksaitis Enhanced capacitative calcium entry and TRPC channel gene expression in human LES smooth muscle Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G1074 - G1083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Tribe, P. Moriarty, A. Dalrymple, A. A. Hassoni, and L. Poston Interleukin-1{beta} Induces Calcium Transients and Enhances Basal and Store Operated Calcium Entry in Human Myometrial Smooth Muscle Biol Reprod, May 1, 2003; 68(5): 1842 - 1849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Golovina, H. Song, P. F. James, J. B. Lingrel, and M. P. Blaustein Na+ pump alpha 2-subunit expression modulates Ca2+ signaling Am J Physiol Cell Physiol, February 1, 2003; 284(2): C475 - C486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, W. G. Wier, and M. P. Blaustein Mg2+ blocks myogenic tone but not K+-induced constriction: role for SOCs in small arteries Am J Physiol Heart Circ Physiol, December 1, 2002; 283(6): H2692 - H2705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Flemming, A Cheong, A M Dedman, and D J Beech Discrete store-operated calcium influx into an intracellular compartment in rabbit arteriolar smooth muscle J. Physiol., September 1, 2002; 543(2): 455 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sweeney, Y. Yu, O. Platoshyn, S. Zhang, S. S. McDaniel, and J. X.-J. Yuan Inhibition of endogenous TRP1 decreases capacitative Ca2+ entry and attenuates pulmonary artery smooth muscle cell proliferation Am J Physiol Lung Cell Mol Physiol, July 1, 2002; 283(1): L144 - L155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Lee, D. Poburko, K.-H. Kuo, C. Y. Seow, and C. van Breemen Ca2+ oscillations, gradients, and homeostasis in vascular smooth muscle Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1571 - H1583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sevieux, J. Alam, and E. Songu-Mize Effect of cyclic stretch on {alpha}-subunit mRNA expression of Na+-K+-ATPase in aortic smooth muscle cells Am J Physiol Cell Physiol, June 1, 2001; 280(6): C1555 - C1560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. McDaniel, O. Platoshyn, J. Wang, Y. Yu, M. Sweeney, S. Krick, L. J. Rubin, and J. X.-J. Yuan Capacitative Ca2+ entry in agonist-induced pulmonary vasoconstriction Am J Physiol Lung Cell Mol Physiol, May 1, 2001; 280(5): L870 - L880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. A. Golovina, O. Platoshyn, C. L. Bailey, J. Wang, A. Limsuwan, M. Sweeney, L. J. Rubin, and J. X.-J. Yuan Upregulated TRP and enhanced capacitative Ca2+ entry in human pulmonary artery myocytes during proliferation Am J Physiol Heart Circ Physiol, February 1, 2001; 280(2): H746 - H755. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Arnon, J. M. Hamlyn, and M. P. Blaustein Ouabain augments Ca2+ transients in arterial smooth muscle without raising cytosolic Na+ Am J Physiol Heart Circ Physiol, August 1, 2000; 279(2): H679 - H691. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
