Am J Physiol Cell Physiol Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 277: C1044-C1049, 1999;
0363-6143/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stevens, L.
Right arrow Articles by Pette, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stevens, L.
Right arrow Articles by Pette, D.
Vol. 277, Issue 6, C1044-C1049, December 1999

EDITORIAL FOCUS
Time-dependent changes in myosin heavy chain mRNA and protein isoforms in unloaded soleus muscle of rat

Laurence Stevens1,2, Karim R. Sultan1, Heidemarie Peuker1, Bärbel Gohlsch1, Yvonne Mounier2, and Dirk Pette1

1 Faculty of Biology, University of Konstanz, D-78457 Konstanz, Germany; and 2 Laboratoire de Plasticité Neuromusculaire, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq, France

Time-dependent changes in myosin heavy chain (MHC) isoform expression were investigated in rat soleus muscle unloaded by hindlimb suspension. Changes at the mRNA level were measured by RT-PCR and correlated with changes in the pattern of MHC protein isoforms. Protein analyses of whole muscle revealed that MHCI decreased after 7 days, when MHCIIa had increased, reaching a transient maximum by 15 days. Longer periods led to inductions and progressive increases of MHCIId(x) and MHCIIb. mRNA analyses of whole muscle showed that MHCIId(x) displayed the steepest increase after 4 days and continued to rise until 28 days, the longest time period investigated. MHCIIb mRNA followed a similar time course, although at lower levels. MHCIalpha mRNA, present at extremely low levels in control soleus, peaked after 4 days, stayed elevated until 15 days, and then decayed. Immunohistochemistry of 15-day unloaded muscles revealed that MHCIalpha was present in muscle spindles but at low amounts also in extrafusal fibers. The slow-to-fast transitions thus seem to proceed in the order MHCIbeta right-arrow MHCIIa right-arrow MHCIId(x) right-arrow MHCIIb. Our findings indicate that MHCIalpha is transiently upregulated in some fibers as an intermediate step during the transition from MHCIbeta to MHCIIa.

hindlimb suspension; messenger ribonucleic acid; myosin heavy chain isoforms; reverse transcriptase-polymerase chain reaction; slow-to-fast transition


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
E. E. Dupont-Versteegden, R. Nagarajan, M. L. Beggs, E. D. Bearden, P. M. Simpson, and C. A. Peterson
Identification of cold-shock protein RBM3 as a possible regulator of skeletal muscle size through expression profiling
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2008; 295(4): R1263 - R1273.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Pierno, J.-F. Desaphy, A. Liantonio, A. De Luca, A. Zarrilli, L. Mastrofrancesco, G. Procino, G. Valenti, and D. Conte Camerino
Disuse of rat muscle in vivo reduces protein kinase C activity controlling the sarcolemma chloride conductance
J. Physiol., November 1, 2007; 584(3): 983 - 995.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
Y. Mounier, V. Montel, F. Picquet, L. Stevens, B. Bastide, and M. Falempin
Dual effect of deafferentation on contractile characteristics and sarcoplasmic reticulum properties in rat soleus fibers
J Appl Physiol, August 1, 2005; 99(2): 542 - 548.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Fluck, S. Schmutz, M. Wittwer, H. Hoppeler, and D. Desplanches
Transcriptional reprogramming during reloading of atrophied rat soleus muscle
Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2005; 289(1): R4 - R14.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
C. Dapp, S. Schmutz, H. Hoppeler, and M. Fluck
Transcriptional reprogramming and ultrastructure during atrophy and recovery of mouse soleus muscle
Physiol Genomics, December 15, 2004; 20(1): 97 - 107.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Bozzo, L. Stevens, L. Toniolo, Y. Mounier, and C. Reggiani
Increased phosphorylation of myosin light chain associated with slow-to-fast transition in rat soleus
Am J Physiol Cell Physiol, September 1, 2003; 285(3): C575 - C583.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. C. Geiger, J. P. Bailey, W.-Z. Zhan, C. B. Mantilla, and G. C. Sieck
Denervation-induced changes in myosin heavy chain expression in the rat diaphragm muscle
J Appl Physiol, August 1, 2003; 95(2): 611 - 619.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. Stevens, C. Bozzo, T. Nemirovskaya, V. Montel, M. Falempin, and Y. Mounier
Contractile properties of rat single muscle fibers and myosin and troponin isoform expression after hypergravity
J Appl Physiol, June 1, 2003; 94(6): 2398 - 2405.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Goto, R. Okuyama, M. Honda, H. Uchida, T. Akema, Y. Ohira, and T. Yoshioka
Profiles of connectin (titin) in atrophied soleus muscle induced by unloading of rats
J Appl Physiol, March 1, 2003; 94(3): 897 - 902.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
F. Picquet, V. Bouet, M. H. Canu, L. Stevens, Y. Mounier, M. Lacour, and M. Falempin
Contractile properties and myosin expression in rats born and reared in hypergravity
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1687 - R1695.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Toursel, L. Stevens, H. Granzier, and Y. Mounier
Passive tension of rat skeletal soleus muscle fibers: effects of unloading conditions
J Appl Physiol, April 1, 2002; 92(4): 1465 - 1472.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. Yamashita-Goto, R. Okuyama, M. Honda, K. Kawasaki, K. Fujita, T. Yamada, I. Nonaka, Y. Ohira, and T. Yoshioka
Maximal and submaximal forces of slow fibers in human soleus after bed rest
J Appl Physiol, July 1, 2001; 91(1): 417 - 424.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. Kischel, B. Bastide, L. Stevens, and Y. Mounier
Expression and functional behavior of troponin C in soleus muscle fibers of rat after hindlimb unloading
J Appl Physiol, March 1, 2001; 90(3): 1095 - 1101.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. Pette
Plasticity in Skeletal, Cardiac, and Smooth Muscle: Historical Perspectives: Plasticity of mammalian skeletal muscle
J Appl Physiol, March 1, 2001; 90(3): 1119 - 1124.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Willmann, J. Kusch, K. R. Sultan, A. G. Schneider, and D. Pette
Muscle LIM protein is upregulated in fast skeletal muscle during transition toward slower phenotypes
Am J Physiol Cell Physiol, February 1, 2001; 280(2): C273 - C279.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Stevens, C. Firinga, B. Gohlsch, B. Bastide, Y. Mounier, and D. Pette
Effects of unweighting and clenbuterol on myosin light and heavy chains in fast and slow muscles of rat
Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1558 - C1563.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Stevens, B. Bastide, P. Kischel, D. Pette, and Y. Mounier
Time-dependent changes in expression of troponin subunit isoforms in unloaded rat soleus muscle
Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1025 - C1030.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online