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Am J Physiol Cell Physiol 277: C392-C402, 1999;
0363-6143/99 $5.00
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Vol. 277, Issue 3, C392-C402, September 1999

Separate taurine and chloride efflux pathways activated during regulatory volume decrease

Andrés Stutzin1, Rubén Torres1, Macarena Oporto1, Patricio Pacheco1, Ana Luisa Eguiguren1, L. Pablo Cid1, and Francisco V. Sepúlveda1,2

1 Facultad de Medicina, Instituto de Ciencias Biomédicas, Universidad de Chile, Santiago 6530499; and 2 Centro de Estudios Científicos de Santiago, Santiago 6760470, Chile

Organic osmolyte and halide permeability pathways activated in epithelial HeLa cells by cell swelling were studied by radiotracer efflux techniques and single-cell volume measurements. The replacement of extracellular Cl- by anions that are more permeant through the volume-activated Cl- channel, as indicated by electrophysiological measurements, significantly decreased taurine efflux. In the presence of less-permeant anions, an increase in taurine efflux was observed. Simultaneous measurement of the 125I, used as a tracer for Cl-, and [3H]taurine efflux showed that the time courses for the two effluxes differed. In Cl--rich medium the increase in I- efflux was transient, whereas that for taurine was sustained. Osmosensitive Cl- conductance, assessed by measuring changes in cell volume, increased rapidly after hypotonic shock. The influx of taurine was able to counteract Cl- conductance-dependent cell shrinkage but only ~4 min after triggering cell swelling. This taurine-induced effect was blocked by DIDS. Differences in anion sensitivity, the time course of activation, and sensitivity to DIDS suggest that the main cell swelling-activated permeability pathways for taurine and Cl- are separate.

cell volume regulation; taurine efflux; iodide efflux; organic osmolyte


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