Am J Physiol Cell Physiol Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Cell Physiol 277: C343-C349, 1999;
0363-6143/99 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Golovina, V. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Golovina, V. A.
Vol. 277, Issue 2, C343-C349, August 1999

Cell proliferation is associated with enhanced capacitative Ca2+ entry in human arterial myocytes

Vera A. Golovina

Department of Physiology, University of Maryland School of Medicine, Baltimore, Maryland 21201

Depletion of Ca2+ stores in the sarcoplasmic reticulum (SR) activates extracellular Ca2+ influx via capacitative Ca2+ entry (CCE). Here, CCE levels in proliferating and growth-arrested human pulmonary artery smooth muscle cells (PASMCs) were compared by digital imaging fluorescence microscopy. Resting cytosolic free Ca2+ concentration ([Ca2+]cyt) in proliferating PASMCs was twofold higher than that in growth-arrested cells. Cyclopiazonic acid (CPA; 10 µM), which inhibits SR Ca2+-ATPase and depletes inositol 1,4,5-trisphosphate-sensitive Ca2+ stores, transiently increased [Ca2+]cyt in the absence of extracellular Ca2+. The addition of 1.8 mM Ca2+ to the extracellular solution in the presence of CPA induced large increases in [Ca2+]cyt, indicative of CCE. The CPA-induced SR Ca2+ release in proliferating PASMCs was twofold higher than that in growth-arrested cells, whereas the transient rise of [Ca2+]cyt due to CCE was fivefold greater in proliferating cells. CCE was insensitive to nifedipine but was significantly inhibited by 50 mM K+, which reduces the driving force for Ca2+ influx, and by 0.5 mM Ni2+, a putative blocker of store-operated Ca2+ channels. These data show that augmented CCE is associated with proliferation of human PASMCs and may be involved in stimulating and maintaining cell growth.

vascular smooth muscle cells; growth factors; sarcoplasmic reticulum; digital imaging; fura 2


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. S Amer, J. Li, D. J. O'Regan, D. S. Steele, K. E. Porter, A. Sivaprasadarao, and D. J. Beech
Translocon closure to Ca2+ leak in proliferating vascular smooth muscle cells
Am J Physiol Heart Circ Physiol, April 1, 2009; 296(4): H910 - H916.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
R. Berra-Romani, A. Mazzocco-Spezzia, M. V. Pulina, and V. A. Golovina
Ca2+ handling is altered when arterial myocytes progress from a contractile to a proliferative phenotype in culture
Am J Physiol Cell Physiol, September 1, 2008; 295(3): C779 - C790.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Yang, X. Ding, and P. A. Murray
Differential effects of intravenous anesthetics on capacitative calcium entry in human pulmonary artery smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, May 1, 2008; 294(5): L1007 - L1012.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
E. R. Resnik, M. Keck, D. J. Sukovich, J. M. Herron, and D. N. Cornfield
Chronic intrauterine pulmonary hypertension increases capacitative calcium entry in fetal pulmonary artery smooth muscle cells
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L953 - L959.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S.-Y. Lee, B.-H. Choi, E.-M. Hur, J.-H. Lee, S.-J. Lee, C. O. Lee, and K.-T. Kim
Norepinephrine activates store-operated Ca2+ entry coupled to large-conductance Ca2+-activated K+ channels in rat pinealocytes
Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1060 - C1066.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Bergdahl, M. F. Gomez, A.-K. Wihlborg, D. Erlinge, A. Eyjolfson, S.-Z. Xu, D. J. Beech, K. Dreja, and P. Hellstrand
Plasticity of TRPC expression in arterial smooth muscle: correlation with store-operated Ca2+ entry
Am J Physiol Cell Physiol, April 1, 2005; 288(4): C872 - C880.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. C. Ng, S. M Wilson, and J. R Hume
Mobilization of sarcoplasmic reticulum stores by hypoxia leads to consequent activation of capacitative Ca2+ entry in isolated canine pulmonary arterial smooth muscle cells
J. Physiol., March 1, 2005; 563(2): 409 - 419.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. Wang, L. G. Laurier, S. M. Sims, and H. G. Preiksaitis
Enhanced capacitative calcium entry and TRPC channel gene expression in human LES smooth muscle
Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G1074 - G1083.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. Bova, L. Trevisi, L. Cima, S. Luciani, V. Golovina, and G. Cargnelli
Signaling Mechanisms for the Selective Vasoconstrictor Effect of Norbormide on the Rat Small Arteries
J. Pharmacol. Exp. Ther., April 13, 2001; 296(2): 458 - 463.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
Y. Wang, J. Chen, Y. Wang, C. W. Taylor, Y. Hirata, H. Hagiwara, K. Mikoshiba, T. Toyo-oka, M. Omata, and Y. Sakaki
Crucial Role of Type 1, but Not Type 3, Inositol 1,4,5-Trisphosphate (IP3) Receptors in IP3-Induced Ca2+ Release, Capacitative Ca2+ Entry, and Proliferation of A7r5 Vascular Smooth Muscle Cells
Circ. Res., February 2, 2001; 88(2): 202 - 209.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
V. A. Golovina, O. Platoshyn, C. L. Bailey, J. Wang, A. Limsuwan, M. Sweeney, L. J. Rubin, and J. X.-J. Yuan
Upregulated TRP and enhanced capacitative Ca2+ entry in human pulmonary artery myocytes during proliferation
Am J Physiol Heart Circ Physiol, February 1, 2001; 280(2): H746 - H755.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online